Lactic Bacterium and Bacillus Sp. Biofilms Can Decrease the Viability of Salmonella Gallinarum, Salmonella Heidelberg, Campylobacter Jejuni and Methicillin Resistant Staphylococcus Aureus on Different Substrates

Monteiro, GP; Rossi, DA; Valadares Jr, EC; Peres, PABM; Braz, RF; Notário, FO; Gomes, MM; Silva, RR; Carrijo, KF; Fonseca, BB

doi:10.1590/1806-9061-2020-1408

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Brazilian Journal of Poultry Science

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Original Article • Braz. J. Poult. Sci. 23 (02) • 2021 • https://doi.org/10.1590/1806-9061-2020-1408 copy

Lactic Bacterium and Bacillus Sp. Biofilms Can Decrease the Viability of Salmonella Gallinarum, Salmonella Heidelberg, Campylobacter Jejuni and Methicillin Resistant Staphylococcus Aureus on Different Substrates

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

This study aimed to evaluate the efficacy of biofilms formed by lactic acid bacteria and Bacillus sp. (BLA) in preventing and controlling the formation of wild biofilms and/or planktonic forms of Salmonella Gallinarum (SG), Salmonella Heidelberg (SH), and methicillin-resistant Staphylococcus aureus (MRSA) on different surfaces. The SH and SG viability was evaluated in polystyrene plates, wood shavings, and soil samples. Two protocols were developed to examine the use of BLA in a preventive and control application. For analysis of Campylobacter jejuni (CJ) BLA was used only preventively in a polystyrene plate. Results showed that BLA was effective in preventing the growth of SG and SH in all matrices. The effectiveness of BLA for MRSA was lower than for SG and SH. The efficiency of BLA in preventing CJ growth seems to be related to the initial CJ contamination. BLA proves to be a potential alternative to control food-borne pathogens commonly encountered in animal production and food industry.

Keywords:
Biocontrol; Polystyrene; Poultry; Soil; Wood shavings

	Initial inoculums	PC	NC	T	PC	NC	T
		24h			48h
Soil (log CFU/g)	2.00^a ± 0.00	8.74^b ± 0.32	0.00^c ± 0.00	8.47^b 0.47	8.92^b 0.47	0.00^c ± 0.00	2.9^a ± 0.97
Wood shavings (log CFU/g)	2.00^a ± 0.00	7.53^b ±0.19	0.00^c ± 0.00	1.00*^d ±0.00	8.68^e 0.23	0.00^c ± 0.00	1.00*^d ±0.00
Polystyrene Plates (log CFU/well)	4.00^a ± 0.00	6.01^b ±0.50	0.00^c ± 0.00	3.42^a ± 0.43	7.27^b ±0.11	0.00^c ± 0.00	3.54^a ±0.31

	Initial inoculums	PC 24h	NC 24h	T 24h	PC 48h	NC 48h	T 48h
Soil (log CFU/g)	2.00^a ±0.00	8.32^b ±0.56	0.00^c ±0.00	8.10^b ±0.24	8.32^b ±0.56	0.00^c ±0.00	7.34^b ±0.67
Wood shavings (log CFU/g)	2.00^a ±0.00	6.32^b ±0.31	0.00^c ±0.00	4.06^d ±0.06	7.04^b ±1.08	0.00^c ±0.00	3.67^d ±0.18
Polystyrene Plates (log CFU/well)	4.00^a ±0.00	6.64^b ±0.80	0.00^c ±0.00	6.40^b ±0.34	8.52^d ±0.33	0.00^c ±0.00	8.31^d ±0.17

	Initial inoculum	PC 24h	NC 24h	T 24h	PC 48h	NC 48h	T 48h
Soil (log CFU/g)	2.00^a ± 0.00	7.31^b ± 0.18	0.00^c ± 0.00	7.73^b ± 0.07	7.16^b ± 0.33	0.00^c ± 0.00	6.68^d ± 0.21
Wood shavings (log CFU/g)	2.00^a ±0.00	4.65^b ± 0.41	0.00^c ±0.00	3.38^d ±0.24	5.45^b ±0.09	0.00^c ± 0.00	4.42^bd ± 0.26
Polystyrene Plates (log CFU/well)	4.00^a ±0.00	10.07^b ±0.17	0.00^c ±0.00	6.53^d ±1.69	10.55^b ±0.17	0.00^c ±0.00	7.16^d ±0.61

	Initial inoculums	PC 24h	NC 24h	T 24h	PC 48h	NC 48h	T 48h
Soil(log CFU/g)	2.00^a ±0.00	9.64^b ±0.36	0.00^c ±0.00	9.60^b ±0.13	8.65^d ±0.69	0.00^c ±0.00	8.87^d ±0.36
Wood shavings (log CFU/g)	2.00^a ±0.00	5.34^b ±0.99	0.00^c ±0.00	4.57^b ±1.27	6.45^b 0.85	0.00^c ±0.00	4.547^b ±1.21
Polystyrene Plates (log CFU/well)	4.00^a ± 0.00	10.0^b ±0.22	0.00^c ±0.00	10.06^b ±0.05	8.824^b ±1.09	0.00^c ±0.00	8.438^b ±1.10

	Initial inoculum	PC 24h	NC 24h	T 24h	PC 48h	NC 48h	T 48h
C.jejuni IAL2383 before BLA	5.43^a ± 0.02	8.3^b ±0.82	0.00^c ±0.00	8.26^b ±1.16	8.00^b ±0.32	0.00^c ±0.00	7.93^b ±0.12
C.jejuni IAL2383 After BLA	5.23^a ± 0.01	5.29^a ± 0.30	0.00^c ± 0.00	6.15^ab ± 0.53	6.65^b ±0.69	0.00^c ±0.00	6.89^b ±0.55

	Ii	PC 24h	NC 24h	T 24h	PC 48h	NC 48h	T 48h	PC 72h	NC 72h	T 72h	PC 96h	NC 96h	T 96h
C. jejuni 596	3.0^a ± 0.00	4.73^bd ± 0.24	0.00^c ± 0.00	4.6^bd ± 0.41	5.75^b ± 0.80	0.00^c ± 0.00	5.95^b ± 0.59	4.69^bd ± 0.45	0.00^c ± 0.00	4.9^bd ± 0.65	5.64^b ± 1.20	0.00^c ± 1.00	3.90^ad ± 0.06
C. jejuni33454/1	3.0^a ± 0.00	4.51^bde ± 0.41	0.00^c ± 0.00	4.12^be ± 0.16	5.17^d ± 0.16	0.00^c ± 0.00	3.78^ae ± 0.57	4.83^bd ± 0.37	0.00^c ± 0.00	3.73^ae ± 0.64	4.28^bd ± 0.00	0.00^c ± 0.00	3.26^a ± 0.07

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[1] Corresponding author e-mail address Raquelline Figueiredo Braz Laboratory of Applied Animal Biotechnology, Faculty of Veterinary Medicine, Federal University of Uberlândia, Ceará Street, 2D Block, Room 43, Umuarama, Uberlândia, Minas Gerais, 38402-018, Brazil. Phone: +55 34 991784578 Email: llinevet@gmail.com