This work aimed to evaluate the control of root-knot nematodes and sugarcane growth in function of Bacillus subtilis in soil after multiplication in vinasse. Laboratory tests were developed to define the best concentration of vinasse on the composition of culture medium to optimize the growth of B. subtilis. For the experiment in the greenhouse, soil collected in the area of sugarcane cultivation was used. The following treatments were established: control, vinasse (50 m³ ha-1), Bacillus subtilis in aqueous suspension (50 m³ ha-1); B. subtilis multiplied in vinasse (50 and 100 m³ ha-1). The multiplication of Bacillus subtilis in pure vinasse (25%) was significantly superior when compared to nutrient solution. The application of B. subtilis in aqueous solution promoted growth of sugarcane and reduced reproduction of the nematodes in the roots during the experiment. The use of B. subtilis, multiplied in vinasse did not achieve the benefits to promote growth and control of nematodes in sugarcane, found with the application of only bacteria in soil.
organic residues; Meloidogyne; phytopathology; biological control
