Antifungal effect of essential oils on control of phytopathogens in stored soybean seeds

Baiotto, Cristiano S.; Baiotto, Luana M. C.; Beber, Simony C.; Kleibert, Karine R. U.; Fell, Ana P. W.; Babeski, Cristhian M.; Bandeira, Willyan J. A.; Basso, Natiane C. F.; Silva, José A. G. da; Colet, Christiane de F.

doi:10.1590/1807-1929/agriambi.v27n4p272-278

Acessibilidade / Reportar erro

Brasil

Revista Brasileira de Engenharia Agrícola e Ambiental

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Articles • Rev. bras. eng. agríc. ambient. 27 (4) • Apr 2023 • https://doi.org/10.1590/1807-1929/agriambi.v27n4p272-278 copy

Antifungal effect of essential oils on control of phytopathogens in stored soybean seeds¹ 1 Research developed at Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Instituto Regional de Desenvolvimento Rural, Augusto Pestana, RS, Brazil

Efeito antifúngico de óleos essenciais no controle de fitopatógenos em sementes de soja armazenadas

Authorship SCIMAGO INSTITUTIONS RANKINGS

HIGHLIGHTS:

The treatment of soybean seeds with essential oil of Rosmarinus officinalis showed efficiency in controlling the storage fungus.

R. officinalis essential oil does not alter the germination of stored soybean seeds of the cultivar Brasmax Lance®.

Soybean seeds treated with Vitavax-Thiram® showed a lower incidence of storage fungi than seeds treated with essential oil.

ABSTRACT

Soybean is subject to occurrences of pathogens transmitted by seeds, requiring phytosanitary treatment, however, it can be toxic to humans and the environment. The objective of this study was to evaluate the effect of essential oils of rosemary (Rosmarinus officinalis) and eucalyptus (Corymbia citriodora) on the control of phytopathogenic fungi in stored soybean seeds. The experiment was carried out in a completely randomized design, using a 2 × 5 factorial arrangement consisted of seeds with and without fungal inoculation (Aspergillus flavus and Penicillium rubens) and five seed treatments (three essential oils, positive control, and negative control), with four replicates. Rosemary and eucalyptus essential oils and their combination were applied to soybean seeds at the rate of 4 mL kg^-1, at a concentration of 700 µL mL^-1. Distilled water with 2% Tween® 80 was used as negative control, and the commercial fungicide Vitavax-Thiram® 200SC was used as positive control, according to the manufacturers’ recommendations. The R. officinalis essential oil was efficient in the control of Penicillium sp. in stored soybean seeds and showed a similar effect to the positive control. The C. citriodora essential oil reduced the incidence of Aspergillus sp. Therefore, these essential oils have potential to be used as an alternative control of soybean seed pathogens.

Key words:
Glycine max (L.) Merril; alternative control of pathogens; Corymbia citriodora; Rosmarinus officinalis; seed treatment

Treatments	Description	Concentration /Rate	Application volume per kg of soybean seed (mL kg^-1)
T1	Seeds without inoculation + sterile distilled water with Tween® 80	-	4
T2	Seeds without inoculation + Rosmarinus officinalis EO	700 µL mL^-1	4
T3	Seeds without inoculation + Corymbia citriodora EO	700 µL mL^-1	4
T4	Seeds without inoculation + Rosmarinus officinalis EO (50%) + Corymbia citriodora EO (50%)	700 µL mL^-1	4
T5	Seeds without inoculation + Vitavax-Thiram® 200SC + sterile distilled water	2 g mL^-1	4
T6	Seeds inoculated with fungi (Aspergillus flavus and Penicillium rubens)	5 x 10⁶ CFU L^-1	-
T7	Seeds inoculated with fungi + Rosmarinus officinalis EO	700 µL mL^-1	4
T8	Seeds inoculated with fungi + Corymbia citriodora EO	700 µL mL^-1	4
T9	Seeds inoculated with fungi + Rosmarinus officinalis EO (50%) + Corymbia citriodora EO (50%)	700 µL mL^-1	4
T10	Seeds inoculated with fungi + Vitavax-Thiram® 200SC+ sterile distilled water	2 g mL^-1	4

Source of variation	Degrees of freedom	Mean square
Source of variation	Degrees of freedom	Germination	Vigor
Inoculation (I)	1	78.4^ns	48.4^ns
Treatments (T)	4	119.0*	260.3*
I × T	4	91.4^ns	71.1^ns
Error	30	43.2	12.02
Overall mean	-	79.0	77.8
Coefficient of variation (%)	-	8.3	8.6

Treatments	Germination	Vigor
Treatments	(%)
Negative control	85.50 a	81.50 a
Positive control*	79.50 b	85.50 a
Rosemary	77.00 b	76.70 b
Eucalyptus	77.00 b	71.50 b
Rosemary + Eucalyptus	76.00 b	73.70 b

Treatments	Germination (%)		Vigor (%)
Treatments	Abnormal seeds	Dead seeds	Abnormal seeds	Dead seeds
Negative control	10.0 ^I	4.5 ^I	11.5	7.0
Positive control**	13.8 ^IS	6.7	10.8 ^I	3.7 ^I
Rosemary	13.3	9.7	13.8	9.5
Eucalyptus	11.8	11.2	13.3	15.2 ^S
Rosemary + Eucalyptus	12.2	11.8	15.8 ^S	10.5
Overall mean	12.2	8.8	13.0	9.2
Coefficient of variation (%)	2.2	9.6	3.9	18.2
Standard deviation (SD)	1.5	3.1	2.0	4.3

Source of variation	Degree of freedom	Mean square
Source of variation	Degree of freedom	Aspergillus sp.	Penicillium sp.	Fusarium sp.	Rhizopus sp.
Inoculation (I)	1	3385.6*	3.6*	1177.2*	1345.6*
Treatments (T)	4	2990.6*	6.4*	1164.5*	776.6*
I × T	4	162.6*	1.6*	615.5*	668.6*
Error	30	51.0	0.46	116.5	105.5
Overall mean	-	75.8	1.1	62.6	84.2
Coefficient of variation (%)	-	9.4	62.1	17.2	12.2

Condition	Treatments/fungal incidence (%)
Condition	Negative control	Rosemary	Eucalyptus	Rosemary + Eucalyptus	Vitavax-Thiram® (Positive control)
	Aspergillus sp.
Without inoculation	82 aC	79 aC	73 bB	69 aB	30 aA
With inoculation	94 bC	95 bC	82 bB	100 bC	54 bA
	Penicillium sp.
Without inoculation	2 aC	1 bB	3 bD	1 aB	0 aA
With inoculation	2 aC	0 aA	1 aB	1 aB	0 aA

Treatments	Fungi (%)
Treatments	Fusarium sp.	Rhizopus sp.
Control	51 a	79 a
Vitavax-Thiram	64 b	71 a
Rosemary	68 b	88 b
Eucalyptus	51 a	87 b
Rosemary + Eucalyptus	79 c	97 b

Unidade Acadêmica de Engenharia Agrícola Unidade Acadêmica de Engenharia Agrícola, UFCG, Av. Aprígio Veloso 882, Bodocongó, Bloco CM, 1º andar, CEP 58429-140, Campina Grande, PB, Brasil, Tel. +55 83 2101 1056 - Campina Grande - PB - Brazil
E-mail: revistagriambi@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[1] * Corresponding author - E-mail: natianeferrari@gmail.com

Brasil

Brasil

Antifungal effect of essential oils on control of phytopathogens in stored soybean seeds1 1 Research developed at Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Instituto Regional de Desenvolvimento Rural, Augusto Pestana, RS, Brazil

Efeito antifúngico de óleos essenciais no controle de fitopatógenos em sementes de soja armazenadas

HIGHLIGHTS:

ABSTRACT

Antifungal effect of essential oils on control of phytopathogens in stored soybean seeds¹ 1 Research developed at Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Instituto Regional de Desenvolvimento Rural, Augusto Pestana, RS, Brazil