Some of the special characteristics of the laser associated with optical phenomena allows a series of applications in the biological area, based on the well-known phenomenon biospeckle. The technique of biospeckle has been developed as a tool for analysis of biological materials activity for the checking of moisture, level of activity, presence of parasites, among other attributes. Although the monitoring occurs by illuminating the material with a source of low power laser, the influence of the light on up the specimen's biological activity becomes always a variable to be investigated. This work had as its objective to evaluation the influence the laser in the analysis of the biological activity measured by the biospeckle of a vegetal tissue; in this case a leaf of coffee plant, using the illumination time as parameter. Two ways of illumination had been used, the first one being reflecting only the laser on the leaf; and the second one the laser reflecting with a source of non-coherent light illuminating the environment. From the experimental results, it was verified that the data of the activity did not have any significant influence with the incidence of the laser, as well as of the non-coherent light throughout the time.
bio-speckle; image analysis; coffee
