Anatomical and histochemical descriptions of the male reproductive system of <i>Diachasmimorpha longicaudata</i> (Ashmed) (Hymenoptera: Braconidae)

The parasitoid Diachasmimorpha longicaudata is an important control agent for several species of fruit flies. Research on the characteristics of the reproductive system and reproductive biology of this parasitoid can be valuable for studies in taxonomy and phylogeny of insects of the order Hymenoptera. In this study we analyzed the histology and histochemistry of the male reproductive system of D. longicaudata. In this species the male reproductive system consists of a pair of testes, two accessory glands, and an ejaculatory duct. Each testicle consists of only one follicle. The testicular follicles are filled with cysts in different stages of spermatogenesis. Histochemical analyses detected proteins and carbohydrates in the cytoplasm of secretory cells and in the lumen of accessory glands. The morphology of the male reproductive system of D. longicaudata differs in some respects from other species of Hymenoptera.

Keywords:
Histology; Spermatogenesis; Accessory glands; Seminal vesicles; Histochemistry

Authorship

Maria das Dores da Cruz Souza ^**Corresponding author. E-mail: souza.maria302@hotmail.com (M.D.C. Souza).

Universidade Estadual de Montes Claros (UNIMONTES), Janaúba, MG, Brasil.Universidade Estadual de Montes ClarosBrasilJanaúba, MG, BrasilUniversidade Estadual de Montes Claros (UNIMONTES), Janaúba, MG, Brasil.

http://orcid.org/0000-0002-9858-2840

Max Pereira Gonçalves

Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Janaúba, MG, Brasil.Universidade Federal dos Vales do Jequitinhonha e MucuriBrasilJanaúba, MG, BrasilUniversidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Janaúba, MG, Brasil.

Edmilson Amaral de Souza

Universidade Federal de Viçosa (UFV), Instituto de Ciências Biológicas e da Saúde, Rio Paranaíba, MG, Brasil.Universidade Federal de ViçosaBrasilRio Paranaíba, MG, BrasilUniversidade Federal de Viçosa (UFV), Instituto de Ciências Biológicas e da Saúde, Rio Paranaíba, MG, Brasil.

Wellen Oliveira Batista

Teresinha Augusta Giustolin

Carlos Gustavo da Cruz

Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brasil.Universidade Federal de Uberlândia BrasilUberlândia, MG, BrasilUniversidade Federal de Uberlândia (UFU), Uberlândia, MG, Brasil.

Clarice Diniz Alvarenga

*Corresponding author. E-mail: souza.maria302@hotmail.com (M.D.C. Souza).

Associate Editor

Juliano Morimoto

Conflicts of interest

The authors declare no conflicts of interest.

Author contribution statement

MDCS: Investigation; Methodology; Data curation; Formal analysis; Writing - original draft; Writing - review & editing. MPG: Conceptualization; Formal analysis; Writing - review & editing. EAS: Investigation; Methodology; Writing - review & editing. WOB: Investigation; Methodology. TAG: Writing - review & editing. CGC: Investigation; Methodology. CDA: Conceptualization; Funding acquisition; Investigation; Methodology; Project administration; Data curation; Supervision; Writing - original draft; Writing - review & editing.

SCIMAGO INSTITUTIONS RANKINGS

Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brasil.Universidade Federal de Uberlândia BrasilUberlândia, MG, BrasilUniversidade Federal de Uberlândia (UFU), Uberlândia, MG, Brasil.

Figures

Figures (5)

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Figure 1
Photomicrograph of the male reproductive system of Diachasmimorpha longicaudata. A. The testes and seminal vesicles are surrounded by the capsule (c), accessory gland (g), ejaculatory duct (ed), B. testicles after capsule rupture. Testis (t), deferent duct (dd), seminal vesicle (sv), accessory glands (g), ejaculatory duct (ed) and aedeagus (ae).

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Figure 2
The general view of the reproductive system of Diachasmimorpha longicaudata. A. The two testicles, are surrounded by a single capsule of connective tissue; B. Each testicle, seminal vesicle and deferent duct are encapsulated separately. Testicle (t), accessory glands (g), ejaculatory duct (ed).

Thumbnail

Figure 3
Histology of the male reproductive system of Diachasmimorpha longicaudata. A. Longitudinal section of the testicle, showing the presence of only one follicle. B. Germarium region with spermatogonia from the testicular follicle (circle). Spermatogonia (Sg). C. Cysts with spermatocytes in the testicular follicle growth zone (circle). Spermatocytes (Sc). D. Testicular follicle with cysts at different stages of spermatogenesis: Cystic cell (arrow continues), spermatids (dashed circle), spermatozoa in bundles with elongated shape (continuous circle), spermatozoa bundles (dotted arrow), conjunctiva capsule (C). Spermatids (St); Spermatozoa (Sz). E. Cyst containing mature spermatozoa (arrow), cystic cell nucleus (CC).

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Figure 4
Histology of the seminal vesicle and the deferent duct of Diachasmimorpha longicaudata. A. Longitudinal section of the seminal tubular vesicle filled with free spermatozoa (sz), B. Cross section of the seminal vesicle, showing cubic epithelial cells (arrow). C. Deferent duct containing free spermatozoa (sz) and pavement epithelial cells (arrow).

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Figure 5
Histochemistry of the accessory glands of Diachasmimorpha longicaudata. A. Longitudinal section and B. cross section of the accessory gland stained with Xylidine Ponceau. Lumen (L), protein secretions (arrow). C. Accessory gland wall containing secretory vesicles (arrow). D and E. PAS. positive for secretions and substances present in the apical region of secretory cells, and pavement epithelial cells (arrow).

Figure 1 Photomicrograph of the male reproductive system of Diachasmimorpha longicaudata. A. The testes and seminal vesicles are surrounded by the capsule (c), accessory gland (g), ejaculatory duct (ed), B. testicles after capsule rupture. Testis (t), deferent duct (dd), seminal vesicle (sv), accessory glands (g), ejaculatory duct (ed) and aedeagus (ae).

Figure 2 The general view of the reproductive system of Diachasmimorpha longicaudata. A. The two testicles, are surrounded by a single capsule of connective tissue; B. Each testicle, seminal vesicle and deferent duct are encapsulated separately. Testicle (t), accessory glands (g), ejaculatory duct (ed).

Figure 3 Histology of the male reproductive system of Diachasmimorpha longicaudata. A. Longitudinal section of the testicle, showing the presence of only one follicle. B. Germarium region with spermatogonia from the testicular follicle (circle). Spermatogonia (Sg). C. Cysts with spermatocytes in the testicular follicle growth zone (circle). Spermatocytes (Sc). D. Testicular follicle with cysts at different stages of spermatogenesis: Cystic cell (arrow continues), spermatids (dashed circle), spermatozoa in bundles with elongated shape (continuous circle), spermatozoa bundles (dotted arrow), conjunctiva capsule (C). Spermatids (St); Spermatozoa (Sz). E. Cyst containing mature spermatozoa (arrow), cystic cell nucleus (CC).

Figure 4 Histology of the seminal vesicle and the deferent duct of Diachasmimorpha longicaudata. A. Longitudinal section of the seminal tubular vesicle filled with free spermatozoa (sz), B. Cross section of the seminal vesicle, showing cubic epithelial cells (arrow). C. Deferent duct containing free spermatozoa (sz) and pavement epithelial cells (arrow).

Figure 5 Histochemistry of the accessory glands of Diachasmimorpha longicaudata. A. Longitudinal section and B. cross section of the accessory gland stained with Xylidine Ponceau. Lumen (L), protein secretions (arrow). C. Accessory gland wall containing secretory vesicles (arrow). D and E. PAS. positive for secretions and substances present in the apical region of secretory cells, and pavement epithelial cells (arrow).

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Métricas

Anatomical and histochemical descriptions of the male reproductive system of <i>Diachasmimorpha longicaudata</i> (Ashmed) (Hymenoptera: Braconidae)

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Mensagem

[1] *Corresponding author. E-mail: souza.maria302@hotmail.com (M.D.C. Souza).