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Forms of sterilization of GA3 and morphogenic reaction in microcuttings of papaya

This study investigated the use of gibberellin (GA3) in medium in vitro propagation of papaya 'Tainung 01' and checking the elongation of shoots and their behavior in the ex vitro rooting.. Shoots less than one cm were subjected to elongation on MS medium containing GA3 treatments as in mg L-1: T1 = 0.0 T2 = 0.5 with sterilized culture medium, T3 = 0.5 sterilized cold, T4 = 2.0 with sterilized culture medium, T5 = 2.0 sterile cold. After these treatments, the shoots were subjected to ex vitro rooting. It was observed that GA3 at levels of 0.5 and 2.0 mg L-1 used in autoclaving after the multiplication has not proved effective for lengthening the shoots, but when sterilized by filtration proved to be effective in lengthening the shoots, however, is harmful in the subsequent stage of rooting and can latter, require subculture to fresh medium supplemented with auxin to induce rooting.

Carica papaya; gibberellin; elongation; ex vitro rooting


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