Morpho-genetic profiling and phylogenetic relationship of guava (<i>Psidium guajava</i> l.) as genetic resources in Pakistan

Kareem, Abdul; Jaskani, Muhammad Jafar; Mehmood, Asim; Khan, Iqrar Ahmad; Awan, Faisal Saeed; Sajid, Muhammad Wasim

doi:10.1590/0100-29452018069

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Revista Brasileira de Fruticultura

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Genética e Melhoramento • Rev. Bras. Frutic. 40 (4) • 2018 • https://doi.org/10.1590/0100-29452018069 copy

Morpho-genetic profiling and phylogenetic relationship of guava (Psidium guajava l.) as genetic resources in Pakistan

Caracterização morfo-genético e relação filogenética de goiaba (Psidium guajava l.) como recursos genéticos no Paquistão

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Guava (Psidium guajava L.) is an open-pollinated crop having 25-40% dissimilarity index which promotes heterozygosity and adds new cultivars. Morpho-genetic characterization of 37 guava accessions was carried out for genetic variability and structure of guava germplasm located in Punjab province, Pakistan. Principal Component analysis (PCA) was subjected to analyze the morphological diversity and for genetic analysis we applied cluster analysis, using the PyElph software. PCA distributed thirty one traits into six components and first two components accounted 39.5% of total variation. A dendrogram constructed on the basis of morphological traits which showed 34% dissimilarity index among thirty seven guava accessions and divided them into 6 groups. For genetic characterization 18 microsatellites were used, the size of reproducible and scorable bands ranged from 150 to 320 bp. The 18 primer pairs amplified 85 alleles with an average number of 4.7 alleles per locus and no more than two displayed bands (nuclear SSR loci). The phylogenetic tree based on molecular analysis showed 50% dissimilarity index among selected guava accessions and separated them into 4 groups.

Index terms
cross pollinated; dendrogram; genetic diversity; reproducible; SSR markers

Sociedade Brasileira de Fruticultura Via de acesso Prof. Paulo Donato Castellane, s/n , 14884-900 Jaboticabal SP Brazil, Tel.: +55 16 3209-7188/3209-7609 - Jaboticabal - SP - Brazil
E-mail: rbf@fcav.unesp.br

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code	Accession name	Botanical name	Native	Phenotypic characteristics
FSDS₁	Gola	P. guajava	Faisalabad	Round (medium size)
FSDS₂	Surahi	P. guajava	Faisalabad	Pear shape (medium size)
FSDS₃	Rough gola	P. guajava	Faisalabad	Round (grooved skin)
FSDS₄	Motagola	P. guajava	Faisalabad	Round (large size)
FSDS₅	Bangladeshi gola	P. guajava	Faisalabad	Round (Extra-large size)
FSDS₆	Lalgola	P. cattleianum	Faisalabad	Round (pink flesh)
FSDS₇	Surahi	P. guajava	Faisalabad	Pear shape (medium size)
FSDS₈	Khatta	P. guajava	Faisalabad	Round (sour taste)
FSDS₉	Surahi	P. guajava	Faisalabad	Pear shape (small size)
FSDS₁₀	Gola	P. guajava	Faisalabad	Round (small size)
FSDS₁₁	Allah Abadi gola	P. guajava	Faisalabad	Round (medium size)
FSDS₁₂	Lalgola	P. cattleianum	Faisalabad	Round (pink flesh)
FSDS₁₃	Karalla	P. guajava	Faisalabad	Pear shape (long neck)
FSDS₁₄	Lalgola	P. cattleianum	Faisalabad	Round (pink flesh)
FSDS₁₅	Gola	P. guajava	Faisalabad	Round (medium size)
FSDS₁₆	Sadabahar gola	P. guajava	Faisalabad	Round (medium size)
FSDS₁₇	Larkanagola	P. guajava	Faisalabad	Round (medium size)
SKPS₁₈	Gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₁₉	Mota gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₂₀	Surahi	P. guajava	Sheikhupura	Pear shape (medium size)
SKPS₂₁	Chota gola	P. guajava	Sheikhupura	Round (small size)
SKPS₂₂	Gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₂₃	Surahi	P. guajava	Sheikhupura	Pear shape (medium size)
SKPS₂₄	Gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₂₅	Surahi	P. guajava	Sheikhupura	Pear shape (medium size)
SKPS₂₆	Gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₂₇	Mota gola	P. guajava	Sheikhupura	Round (large size)
SKPS₂₈	Lalgola	P. cattleianum	Sheikhupura	Round (pink flesh)
SKPS₂₉	Choti surahi	P. guajava	Sheikhupura	Pear shape (small size)
SKPS₃₀	Larkana gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₃₁	Desi gola	P. guajava	Sheikhupura	Round (small size)
SKPS₃₂	Gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₃₃	Mota gola	P. guajava	Sheikhupura	Round (large size)
SKPS₃₄	Moti surahi	P. guajava	Sheikhupura	Pear shape (large size)
SKPS₃₅	Gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₃₆	Sadabahar gola	P. guajava	Sheikhupura	Round (medium size)
SKPS₃₇	Sadabahar gola	P. guajava	Sheikhupura	Round (medium size)

Sr. No.	Primer	Forward Sequence	Reverse sequence	Ta (°C)	No. of Alleles	Allele size range
1	mPgCIR02	AGTGAACGACTGAAGACC	ATTACACATTCAGCCACTT	55	5	232-260
2	mPgCIR03	TTGTGGCTTGATTTCC	TCGTTTAGAGGACATTTCT	55	2	202-220
3	mPgCIR05	GCCTTTGAACCACATC	TCAATACGAGAGGCAATA	55	5	230-295
4	mPgCIR07	ATGGAGGTAGGTTGATG	CGTAGTAATCGAAGAAATG	55	6	160-170
5	mPgCIR08	ACTTTCGGTCTCAACAAG	AGGCTTCCTACAAAAGTG	55	5	215-235
6	mPgCIR09	GCGTGTCGTATTGTTTC	ATTTTCTTCTGCCTTGTC	55	4	160-185
7	mPgCIR10	GTTGGCTCTTATTTTGGT	GCCCCATATCTAGGAAG	55	4	265-290
8	mPgCIR11	TGAAAGACAACAAACGAG	TTACACCCACCTAAATAAGA	55	5	298-320
9	mPgCIR14	TAAACACAACAAGGGTCA	CAGTTTTCATATCGTCCTC	55	5	190-200
10	mPgCIR15	TCTAATCCCCTGAGTTTC	CCGATCATCTCTTTCTTT	55	5	240-270
11	mPgCIR16	AATACCAGCAACACCAA	CATCCGTCTCTAAACCTC	55	5	270-300
12	mPgCIR17	CCTTTCGTCATATTCACTT	CATTGGATGGTTGACAT	55	3	150-240
13	mPgCIR18	TAAGCTGCATGTGTGC	ATGGCTTTGGATGAAA	55	5	180-200
14	mPgCIR19	AAAATCCTGAAGACGAAC	TATCAGAGGCTTGCATTA	55	3	265-280
15	mPgCIR20	TATACCACACGCTGAAAC	TTCCCCATAAACATCTCT	55	6	275-300
16	mPgCIR21	TGCCCTTCTAAGTATAACAG	AGCTACAAACCTTCCTAAA	55	5	250-285
17	mPgCIR22	CATAAGGACATTTGAGGAA	AATAAGAAAGCGAGCAGA	55	5	200-278
18	mPgCIR25	GACAATCCAATCTCACTTT	TGTGTCAAGCATACCTTC	55	6	170-200