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Morphological and molecular characterization of mangaba genotypes

The objective was to assess the genetic diversity among 25 Mangaba genotypes in the Iramaia-Chapada Diamantina region, Bahia, using morphological markers and RAPD markers. The following measurements were performed in mature fruits: longitudinal (LD) and transverse (TD) diameter, fruit (FM), seed (SM) and the pulp (PM) mass, pulp yield (% P) and the count seed number (SN). The chemical characters were evaluated in mature fruit: pH, acidity (Ac), total soluble solids (TSS), ascorbic acid (AA), total sugar (TS) reducing sugar (RS), non-reducing sugar (NRS) and total soluble solids /acidity (TSS/Ac). For amplification were tested 51 random primers. The average results of the measurements were LD (37.03 mm), TD (34.34 mm), FM (24.45 g), SM (2.42 g), PM (22.03 g), and SN (11.14). For chemical evaluations were checked the average results: % P (88.44%), AA (80.23 mg100g-1), Ac (0.96%), pH (3.67), TSS (14.15 ºBrix) TS (9.26 mg100g-1), RS (5.13 mg100g-1), NRS (4.13 mg100g-1) and TSS/Ac (14.56 mg100g-1). Eight random primers were selected for having more intense bands and six (OPA 08, OPB 12, OPB 19, OPH 15, OPH 18, OPH 19) were sufficient to generate 28 polymorphic bands. Among the morphological markers, FM and PM were the most effectively contributed to divergence. The formation of six and seven groups for molecular markers and morphological, respectively, indicated the presence of genetic variability in the population. .

Hancornia speciosa Gomes; RAPD; genetic diversity


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