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Ultrastructural changes in the cell wall of mango 'Tommy Atkins' treated with calcium chloride at pre-harvest time

Mango 'Tommy Atkins ' produced in Ibirá, São Paulo, was treated at preharvest time with concentrations of 0,0%, 2,5% and 5,0% calcium chloride at three times of its development (40, 60 and 90 days after antesis) in order to identify the influence of calcium on the structure of the cell wall, by using transmission electron microscopy (TEM), immediately after the harvest and at 35 days of storage. The methodology described by Jacob and Gowanlock (1995) was used to fix the pulp material. It was observed that untreated fruits (without calcium chloride) showed desintegration of the cell wall and breakup of the middle lamella (LM) at the harvest time. The degradation of the LM site starts with the formation of quite different empty spaces, whose breakups are larger when prolonging storage for 35 days. The treated fruits with calcium chloride at 5,0% presented a defined LM and absence of empty spaces, even after storage. The application of calcium chloride at 5,0% was effective to preserve the middle lamella structure.

mango; ripening; middle lamella; storage


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