Embryos obtained from Citrus sinensis Osbeck 'Pêra Rio' x Citrus reticulata Blanco 'Poncã' crossings were excised in diverse phases of development and cultivated in different medium concentrations. Embryos in globular, torped and cotiledonary stage were excised and inoculated individually in test tubes with 15 mL in distinct MT medium concentrations (0; 50; 100 and 150% of original composition and added sucrose (50 g.L-1). After inoculation, the embryos were maintained in growth room at 27±1ºC, 16 hour photoperiod and 32 umol.m-2.s-1 irradiance. After 90 days, the length of the aerial part and roots system, fresh mass and number of leaves, were evaluated. The best results were obtained by incubating embryos in the cotiledonary stage, in MT medium with concentrations of 150%.
Citrus sinensis; Citrus reticulata; culture medium; embryos culture
