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Influence of the composition of encapsulation matrix of microshoots of banana (Musa sp.) cv. Prata-Anã Gorutuba clone

Banana is the main fruit in the international trade and the most popular one in the world. The production of synthetic seeds comes highlighting itself as an important technique for the micropropagation and in vitro conservation of several species. The aim of this study was to evaluate the influence of the composition of the encapsulation matrix in the conversion of microshoots of banana cv. Prata Anã Gorutuba clone. The microshoots of banana cv. Prata-Anã Gorutuba clone were encapsulated being used in the sodium alginate matrix: water and MS (50 % and 100 %), added or not with active charcoal (1.5 g L-1) in two evaluation periods (15 and 30 days). Three levels of BAP (0, 3, 6 mg L-1) added or not to 2.5 mg L-1 of NAA were tested in two evaluation periods (15 and 30 days). The two experiments were entirely at random, being a factorial 3 x 2 x 2, with six repetitions and five encapsulated units per plot. On the fifteenth day evaluated the conversion, on the 30th day evaluated the conversion, the height and the rooting of the plants. The capsule constitution, when different MS medium concentrations were used, with or without the presence of active charcoal, did not influence the conversion rate nor plants height. The capsules without addition of growth regulators resulted in better microshoots conversion; however concentration of 3 mg L-1 BAP+2.5 mg L-1 NAA showed higher plant height. The strike rate is higher in capsules containing 100% MS, and active charcoal, and the capsules containing 0 and 3 mg L-1 BAP, regardless of the addition of NAA. On the 30th day greater conversion of synthetic seeds is obtained.

Synthetic seed; Musa sp.; plant growth regulators; active charcoal


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