Hypericum brasiliense Choisy, plant of the Guttiferae family, occurs mainly in the regions Southeastern and South of Brazil. Xanthones, phloroglucinols, flavonoids and other compounds were isolated from this species. There are not registrations in the literature of the chemical composition of the essential oil of this species, in spite of the importance of these compounds in the chemistry of the genera. In this work, it was done the chemical study of the volatile fraction obtained by hidrodestilation of H. brasiliense and this fraction was analyzed by CG/MS.