The purposes of this study were to establish a protocol for the analysis of minisatellites or VNTRs and microsatellites or STRs in patients who undergo allogeneic bone marrow transplantation; to verify the validity of the methodology and of the studied loci and to verify the type of recovery of the patient. The pre-transplant and post-transplant DNA of 14 recipients and their respective donors were analyzed. Six loci were amplified by PCR (D1S80, SE33, HumTH01, 33.6, HumARA and HumTPO). The amplified products were separated using a vertical polyacrylamide gel electrophoretic system and the resulting fragments were analyzed using silver staining. This procedure proved to be efficient in verifying autologous and allogeneic regeneration and probably chimerism regeneration too. Of the loci studied, 63.1% presented results which could be analyzed, 19% were informative results, 13.1% partially informative and 31.0% non-informative. The locus that most demonstrated the informative results was the SE33 locus, the one that showed the partially informative results was the HumTPO and the non-informative results was the HumTH01. The 33.6 locus was the one that presented the majority of the results that could not be tested. Furthermore, it was possible to determine the patient regeneration after transplantation in 71.4% of the cases, 90% showed allogeneic regeneration and 10% autologous regeneration.
VNTRs; STRs; bone marrow transplantation; PCR
