DUATHLON TRAINING MODEL ADAPTED FOR FEMALE RATS: BLOOD GLUCOSE-LACTATE CORRELATIONS

Vasconcelos, Francisco Sérgio Lopes; Souza, Mateus Bastos de; Fortes, Jefferson Pacheco Amaral; Souza, Karla Camila Lima de; Carneiro, Mayara Rangel Araújo; Santos-Júnior, Francisco Fleury Uchoa; Ceccatto, Vânia Marilande

doi:10.1590/1517-869220192501181887

Francisco Sérgio Lopes Vasconcelos Filho

(Physical Education Professional)

Universidade Estadual do Ceará, Fortaleza, CE, Brazil.

http://orcid.org/0000-0003-3140-0085

Mateus Bastos de Souza

(Physiotherapy Student)

Universidade Federal do Ceará, Fortaleza, CE, Brazil.

http://orcid.org/0000-0003-1074-9229

Jefferson Pacheco Amaral Fortes

(Physiotherapist)

Universidade Estadual do Ceará, Fortaleza, CE, Brazil.

Instituto Le Santé, Fortaleza, CE, Brazil.

http://orcid.org/0000-0002-3225-6532

Karla Camila Lima de Souza

(Physiotherapist)

Universidade Estadual do Ceará, Fortaleza, CE, Brazil.

http://orcid.org/0000-0001-9477-6229

Mayara Rangel Araújo Carneiro

(Physiotherapist)

Centro Universitário Estácio do Ceará, Fortaleza, CE, Brazil.

http://orcid.org/0000-0002-5457-3109

Francisco Fleury Uchoa Santos-Júnior

(Physiotherapist)

Instituto Le Santé, Fortaleza, CE, Brazil.

Centro Universitário Estácio do Ceará, Fortaleza, CE, Brazil.

http://orcid.org/0000-0002-5685-4297

Vânia Marilande Ceccatto Correspondence: Vânia Marilande Ceccatto, Biochemistry and Gene Expression Laboratory, Instituto Superior de Ciências Biomédicas. Av. Dr. Silas Munguba, 1700, Campus do Itaperi. Fortaleza, CE, Brazil. 60714-903. vania.ceccatto@uece.br

(Biologist)

Universidade Estadual do Ceará, Fortaleza, CE, Brazil.

http://orcid.org/0000-0003-4839-4400

Correspondence: Vânia Marilande Ceccatto, Biochemistry and Gene Expression Laboratory, Instituto Superior de Ciências Biomédicas. Av. Dr. Silas Munguba, 1700, Campus do Itaperi. Fortaleza, CE, Brazil. 60714-903. vania.ceccatto@uece.br

All authors declare no potential conflict of interest related to this article

AUTHORS’ CONTRIBUTIONS: Each author made significant individual contributions to this manuscript. FSLVF (0000-0003-3140-0085)*: Experimental execution of the study, analysis of results and writing; MBS (0000-0003-1074-9229)*: Experimental execution of the study, analysis of results and writing; JPAF (0000-0002-3225-6532)*: Experimental execution of the study; KCLS (0000-0001-9477-6229)*: Methodological supervision, analysis of results and formatting of articles; MRAC (0000-0002-5457-3109)*: Experimental execution of the study and translation of the article; FFUSJ (0000-0002-5685-4297)*: Experimental project, writing and methodological supervision; VMC (0000-0003-4839-4400)*: methodological supervision. *ORCID (Open Researcher and Contributor ID).

Day	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
Week	Duration (min)	Duration (min)	Duration (min)	Duration (min)	Duration (min)	Duration (min)	Rest
1st	5 min	10 min	15 min	15 min	15 min	15 min	Rest
2nd	20 min	25 min	30 min	30 min	30 min	30 min	Rest
3rd	30 min	30 min	30 min	30 min	30 min	30 min	Rest
4th	30 min	30 min	30 min	30 min	30 min	30 min	Rest

Day	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
Week	Duration (min) S^* * Swimming; /R^ Running.	Duration (min) S/R	Duration (min) S/R	Duration (min) S/R	Duration (min) S/R	Duration (min) S/R	Rest
1st	2m30s –S/2m30s – R (5 min)	5m – S/5m – R (10min)	7m30s – S/7m30s – R (15min)	7m30s – S/7m30s – R (15min)	7m30s – S/7m30s – R (15min)	7m30s – S/7m30s – R (15min)	Rest
2nd	10m – S/10m – R (20min)	12m30s – S/12m30s – R (25min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	Rest
3rd	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	Rest
4th	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	15m – S/15m – R (30min)	Rest

Groups	Control Group		Running Group		Swimming Group		Swimming/Running
	Pre	Post	Pre	Post	Pre	Post	Pre	Post
Blood Glucose
Week 1	97.28 ± 4.69	70.85 ± 5.84^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where	99.42 ± 1.43	90.57 ± 2.81	109.28 ± 3.23	46.65 ± 1.76^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ^b b is relative to the running group, and	110.4 ± 19.54	51.8 ± 10.26 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^b b is relative to the running group, and
Week 2	98.71 ± 4.89	63.14 ± 7.42^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where	91.83 ± 0.75	101.82 ± 1.13^a a indicates difference from the control,	92.15 ± 1.19	33.33 ± 1.22^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ^b b is relative to the running group, and	109.57 ± 7.25	66.28 ± 14.58 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where , ^a a indicates difference from the control, , ^c c is related to the swimming group in the same stage. (pre-training and post-training), p<0.01.
Week 3	103.71 ± 1.76	89.28 ± 9.22	107.57 ± 1.33	116.14 ± 1.96	107.82 ± 3.75	46.33 ± 1.3 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^a a indicates difference from the control, ,^b b is relative to the running group, and	90.71 ± 8	52 ± 9.39 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where , ^a a indicates difference from the control, , ^b b is relative to the running group, and
Week 4	106 ± 1.04	84 ± 2.57^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where	91.71 ± 1.09	93.85 ± 1.47	97.82 ± 0.81	37.83 ± 0.9 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^a a indicates difference from the control, ,^b b is relative to the running group, and	113.57 ± 5.58	42.85 ± 6.58 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where , ^a a indicates difference from the control, , ^b b is relative to the running group, and
Blood Lactate
Week 1	1.47 ± 0.08	3.71 ± 0.24^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where	0.51 ± 0.07^a a indicates difference from the control,	0.95 ± 0.13 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ^a a indicates difference from the control,	0.83 ± 0.12 ^a a indicates difference from the control, ,^b b is relative to the running group, and	3.06 ± 0.33 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^b b is relative to the running group, and	1.46 ± 0.28 ^b b is relative to the running group, and ,^c c is related to the swimming group in the same stage. (pre-training and post-training), p<0.01.	3.47 ± 0.61^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^b b is relative to the running group, and
Week 2	1.17 ± 0.09	2.56 ± 0.18^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where	0.82 ± 0.19	0.85 ± 0.12 ^a a indicates difference from the control,	0.96 ± 0.17	3.13 ± 0.35 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^b b is relative to the running group, and	1.22 ± 0.25^b b is relative to the running group, and	4.14 ± 0.17 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^a a indicates difference from the control, ,^b b is relative to the running group, and
Week 3	0.81 ± 0.13	2.03 ± 0.32	0.79 ± 0.12	0.50 ± 0.07 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ^a a indicates difference from the control,	0.95 ± 0.13	4.58 ± 0.18 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^a a indicates difference from the control, ,^b b is relative to the running group, and	1.48 ± 0.04 ^a a indicates difference from the control, ,^b b is relative to the running group, and	5.46 ± 0.27 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^a a indicates difference from the control, , ^b b is relative to the running group, and
Week 4	1.37 ± 0.04	3.42 ± 0.11^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where	0.45 ± 0.07^a a indicates difference from the control,	0.53 ± 0.03^a a indicates difference from the control,	0.48 ± 0.04 ^a a indicates difference from the control, ,^b b is relative to the running group, and	2.58 ± 0.20 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where ,^b b is relative to the running group, and	1.45 ± 0.15 ^b b is relative to the running group, and ,^c c is related to the swimming group in the same stage. (pre-training and post-training), p<0.01.	4.19 ± 0.23 ^* * Indicates statistical difference between post-training values and pre-training within the same group. The ANOVA two-way test post hoc Bonferroni indicates statistical difference in glucose and lactate concentrations (pre-workout and post-workout), where , ^b b is relative to the running group, and , ^c c is related to the swimming group in the same stage. (pre-training and post-training), p<0.01.

[1] Correspondence: Vânia Marilande Ceccatto, Biochemistry and Gene Expression Laboratory, Instituto Superior de Ciências Biomédicas. Av. Dr. Silas Munguba, 1700, Campus do Itaperi. Fortaleza, CE, Brazil. 60714-903. vania.ceccatto@uece.br

Brasil

Brasil

DUATHLON TRAINING MODEL ADAPTED FOR FEMALE RATS: BLOOD GLUCOSE-LACTATE CORRELATIONS

MODELO DE TREINAMENTO DE DUATHLON ADAPTADO PARA RATAS: CORRELAÇÃO DE GLICOSE-LACTATO NO SANGUE

MODELO DE ENTRENAMIENTO DE DUATLÓN ADAPTADO PARA RATONES: CORRELACIÓN DE GLUCOSA-LACTATO EN LA SANGRE

ABSTRACT

Objective:

Methods:

Results:

Conclusion: