THE RELIEVING EFFECT OF SUN-DRIED PROTEIN TONIC ON SPORTS FATIGUE

Fatigue is a comprehensive process that involves many physiological and biochemical factors. It is a normal physiological reaction when human physical or mental activities reach a certain level. In recent years, it has been verified that free radicals are closely related to exercise-induced fatigue. Cardamine bursa purified selenoprotein has good oxygen-free radical scavenging ability and anti-lipid peroxide. It could protect mitochondria, liver, and red blood cells from peroxide injury. Therefore, it was speculated that the purification of selenoprotein Cardamine may play an active role in attenuating exercise-induced fatigue by scavenging free radicals. This study cleared the selenite protein Capsella bursa (SPC) as a research object, and evaluated its structural characteristics in relieving exercise-induced fatigue. The selenoprotein index system for exercise-induced fatigue was constructed by combining two AHP methods, principal component analysis and factor analysis. Purity, subunit composition, amino acid composition and RCM content were evaluated. The corresponding RCM protein was preliminarily predicted. The results showed that SPCH could significantly prolong the swimming time (P <0.01), improve the lactate clearance capacity (P <0.01), increase the glycogen content of the liver (P <0.01), and reduce the level of the BUN (P <0.05). SPCH has a good effect in relieving exercise-induced fatigue in mice, so it can be considered for development as a nutritional supplement to alleviate exercise-induced fatigue.

Capsella capsulosa; selenoprotein; Physical exercise; Fatigue

group	Number of mice	Prolongation rate of average swimming time / min in female mice	Average swimming time / min prolongation rate of male mice%
Blank control group	10	88.40±5.03##	95.40±0.89##
Positive control group	10	131.60±11.15** 48.87	138.60±4.16** 45.28
Water extract group	10	130.00±7.31** 47.06	133.40±10.57** 39.83
Spch low dose group	9	132.20±8.73** 49.55	136.75±9.07** 43.34
Spch medium group	10	155.80±8.53**## 76.24	199.40±4.22**## 109.01
Spch high dose group	10	163.0±12.33**## 84.39	192.20±9.78**## 101.47
Sodium selenite group	9	94.50±5.07## 6.90	106.20±16.48## 11.32

Group	Number of mice	BLA content in female micet/(mmol/L)	BLA content in male mice/(mmol/L)
Blank control group	9	18.44±2.14••	21.44±2.07••
Positive control group	10	13.25±3.25**	16.54±2.61**
Water extract group	10	14.33±2.15*	19.87±0.55•
Spch low dose group	9	15.52±2.68	15.76±1.18**
Spch medium group	10	12.76±3.09**	14.50±3.50**
Spch high dose group	10	13.96±1.41*	15.82±1.51**
Sodium selenite group	9	16.72±2.23	17.15±0.66**

group	Glycogen content in liver of female rats/(mg/g)	Liver glycogen content of male rats/(mg/g)	Bun content of female rats/(mmol/L)	Bun content in male rats/(mmol/L)
Blank control group	11.56±1.03##	10.60±1.30##	8.85±1.78	8.93±0.15
Positive control group	16.41±1.58**	14.27±2.06**	7.76±0.50	8.32±1.13
Water extract group	12.23±1.20	12.31±1.24	5.66±0.22**##	6.99±0.76**#
Spch low dose group	12.55±0.78##	11.69±1.39#	7.98±0.60	8.44±0.71
Spch medium dose group	15.02±1.33**	13.56±1.76**	7.74±1.49	8.03±0.36
Spch high dose group	17.22±1.66**	15.73±2.23**	7.84±0.66	7.98±0.62
Sodium selenite group	10.66±1.42##	11.93±1.64#	8.65±0.92	8.63±1.74

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[1] Correspondence: Yangling, 712100, Shanxi, China. gft591267@21cn.com