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Original Articles • Rev. bras. ortop. 51 (1) • jan-feb 2016 • https://doi.org/10.1016/j.rboe.2015.12.013   copy

Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes☆ ☆ Work developed at the Instituto Nacional de Traumatologia e Ortopedia (INTO), Rio de Janeiro, RJ, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

OBJECTIVE:

This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract.

METHODS:

Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR.

RESULTS:

The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression.

CONCLUSION:

Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.

Keywords:
Tendon; Mesenchymal stromal cells from bone marrow; Tenocytes

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