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Analysis of β-ecdysone from in vivo and in vitro cultured plants of Pfaffia glomerata (Spreng.) Pedersen using Thin-Layer Chromatography

Pfaffia glomerata (Spreng.) Pedersen, known as Brazilian ginseng, is a plant extensively used in folk medicine due to its phytotherapic characteristics. The aim of this study was to evaluate the presence of β-ecdysone in the roots and shoots of in vivo and in vitro cultured plants of two sources (BRA and JB-UFSM) of Pfaffia glomerata using Thin-Layer Chromatography (TLC). For in vivo cultured plants, TLC plates showed that β-ecdysone was present in the roots and shoots. Sources showed no differences concerning TLC plates. TLC analysis did not detect β-ecdysone in the in vitro cultured plants.

Brazilian ginseng; TLC; ecdysteroids; quality control; in vitro culture


Sociedade Brasileira de Plantas Medicinais Sociedade Brasileira de Plantas Medicinais, Revista Brasileira de Plantas Medicinais, Universidade Estadual de Maringá, Departamento de Farmácia, Bloco T22, Avenida Colombo, 5790, 87020-900 - Maringá - PR, Tel: +55-44-3011-4627 - Botucatu - SP - Brazil
E-mail: revista@sbpmed.org.br