Anthelmintic effect of <i>Cassia fistula</i> and <i>Combretum leprosum</i> protein fractions against goat gastrointestinal nematodes

Silva, Mara Thais de Oliveira; Maia, Mara Andrade Colares; Silva, Michele Dalvina Correia da; Torres, Taffarel Melo; Pereira, José Carlos da Silveira; Araújo, João Ronielly Campêlo; Silva, Ryan Emiliano da; Souza, Anna Lopes da Costa; Barbosa, Tallyson Nogueira; Bezerra, Ana Carla Diógenes Suassuna

doi:10.1590/S1984-296120180041

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Short Communication • Rev. Bras. Parasitol. Vet. 27 (02) • Apr-Jun 2018 • https://doi.org/10.1590/S1984-296120180041 copy

Anthelmintic effect of Cassia fistula and Combretum leprosum protein fractions against goat gastrointestinal nematodes

Efeito antihelmíntico das frações proteicas de Cassia fistula e Combretum leprosum contra nematodeos gastrintestinais de caprinos

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

In this study, we evaluated the ovicidal and larvicidal activity of protein preparations obtained from Cassia fistula L. and Combretum leprosum Mart. leaves on the gastrointestinal parasites of goats. Protein preparations were obtained after the extraction of C. fistula L. and C. leprosum Mart. leaves, followed by protein fractionation (with ammonium sulfate saturation percentages of 30%, 30%-60%, and 60%-90%) and dialysis, which resulted in protein fractions (called F1, F2, and F3, respectively). The fractions were evaluated by egg hatching (the eggs were recovered in stool samples from naturally infected goats) and larval development tests. The results reveled that the inhibition of hatching of eggs caused by the protein fractions of C. fistula (38%) were similar to that of the control drug, thiabendazole. In addition, the fractions of C. fistula caused significant inhibition (61-69%) of larval development also. However, C. leprosum did not reveal significant inhibition of egg hatching and larval development. We conclude that C. fistula L. showed better ovicidal and larvicidal activity against endoparasites.

Keywords:
Phytotherapy; Cassia fistula L.; Combretum leprosum Mart.; larvicidal activity; ovicidal activity

Treatments		*EHT (%) ± SD (%) ^ ** The values represent the mean ± standard deviation (SD). F1, F2, and F3: protein fractions obtained by the saturation of plant extracts with ammonium sulfate (at 30%, 30%-60%, and 60%-90%, respectively), followed by centrifugation and dialysis. Different letters indicate statistically significant differences (P<0.05) when compared with the control.	*LDT (%) ± SD (%) ^ ** The values represent the mean ± standard deviation (SD). F1, F2, and F3: protein fractions obtained by the saturation of plant extracts with ammonium sulfate (at 30%, 30%-60%, and 60%-90%, respectively), followed by centrifugation and dialysis. Different letters indicate statistically significant differences (P<0.05) when compared with the control.
*C. fistula*	F1	38 ± 15 ^a	61 ± 14 ^a
	F2	38 ± 17 ^a	62 ± 26 ^a
	F3	38 ± 17 ^a	69 ± 23 ^a
Controls	H₂O	8 ± 7 ^b	18 ± 7 ^b
	Thiabendazol	30 ± 15 ^a	-
	Ivermectin	-	81 ± 11 ^a

Treatments		*EHT (%) ± SD (%) ^ ** The values represent the mean ± standard deviation (SD). F1, F2, and F3: protein fractions obtained by the saturation of plant extracts with ammonium sulfate (at 30%, 30%-60%, and 60%-90%, respectively), followed by centrifugation and dialysis. Different letters indicate statistically significant differences (P<0.05) when compared with the control.	*LDT (%) ± SD (%) ^ ** The values represent the mean ± standard deviation (SD). F1, F2, and F3: protein fractions obtained by the saturation of plant extracts with ammonium sulfate (at 30%, 30%-60%, and 60%-90%, respectively), followed by centrifugation and dialysis. Different letters indicate statistically significant differences (P<0.05) when compared with the control.
*C. leprosum*	F1	8 ± 23 ^a	18 ± 16 ^a
	F2	10 ± 21 ^a	16 ± 9 ^a
	F3	9 ± 24 ^a	18 ± 10 ^a
Controls	H₂O	4 ± 3 ^a	13 ± 3 ^a
	Thiabendazol	49 ± 30 ^b	-
	Ivermectin	-	57 ± 7 ^b

Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br

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[1] * Corresponding author: Mara Thais de Oliveira Silva. Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas – UFPel, Campus Universitário, s/n, Prédio 19, CEP 96010-900, Pelotas, RS, Brasil. e-mail: marathaisos@gmail.com