<i>Globocephalus urosubulatus</i> (Alessandrini, 1909) (Nematoda: Ancylostomatidae) in Brazil: a morphological revisitation

Pinheiro, Raul Henrique da Silva; Melo, Sérgio; Benigno, Raimundo Nonato Moraes; Giese, Elane Guerreiro

doi:10.1590/S1984-29612021078

Raul Henrique da Silva Pinheiro

Programa de Pós-graduação em Sociedade, Natureza e Desenvolvimento, Instituto de Biodiversidade e Florestas, Universidade Federal do Oeste do Pará – UFOPA, Santarém, PA, Brasil

http://orcid.org/0000-0003-3221-5017

Sérgio Melo

Programa de Pós-graduação em Sociedade, Natureza e Desenvolvimento, Instituto de Biodiversidade e Florestas, Universidade Federal do Oeste do Pará – UFOPA, Santarém, PA, Brasil

http://orcid.org/0000-0002-9315-7044

Raimundo Nonato Moraes Benigno

Laboratório de Parasitologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, Brasil

http://orcid.org/0000-0002-6936-6321

Elane Guerreiro Giese ^**Corresponding author: Elane Guerreiro Giese. E-mail: lheaufra@gmail.com

Laboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, Brasil

http://orcid.org/0000-0001-7833-1334

*Corresponding author: Elane Guerreiro Giese. E-mail: lheaufra@gmail.com

Figures (3)
Tables (1)

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Figure 1
Light microscopy of Globocephalus urosubulatus: (a) Lateral view of cephalic region of female showing buccal capsule (bc); excretory pore (ep); posterior region of the esophagus is expanded and opens into the intestine by a strong valve (v) and intestine (in). Bar = 200µm; (b)Ventral view of cephalic region showing buccal capsule; teeth (te); nerve ring (nr); deirids (de); muscular esophagus (me) and intestine (in). Bar = 100µm; (c) Detail of circular oral opening (*) and rib with small spines reaching the inside tip of the buccal capsule on the back (arrow points). Bar = 100µm; (d) buccal capsule armed with two sclerotized tooth-like structures (arrow points). Bar = 50µm; (e) Detail of vulva. Bar = 100µm; (f) Posterior end of female lateral view: rectum (re) and anus (an). Bar = 50 µm; (g) Detail of eggs. Bar = 50µm; (h) Posterior end of male, lateral view spicules (sp); pre-bursal papillae (pb); Gubernaculum (gu) and papillae a = ventral and lateroventral rays, b = anterolateral, mediolateral, posterolateral rays, c = externodorsal rays and d = dorsal trunk. Bar = 100µm; (i) Overview of a male specimen. Bar = 12µm; (j) Detail of the trifurcated final part of the dorsal trunk. Bar = 50µm.

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Figure 3
Scanning electron microscopy of Globocephalus urosubulatus. (a) Lateral view of cephalic region of female showing circular oral opening, excretory pore (ep) and deirid (de). Bar = 100 µm; (b) Frontal view of the oral opening (*) of males. Bar = 20 µm; (c) View of the rib with small spines reaching the inside tip of the buccal capsule on the back. Bar = 50 µm; (d) Detail of Vulva (vu). Bar = 20µm; (e) Posterior end of female ventral view anus (an), lip elevated (*) and on pair of papillae lateral (arrow points). Bar = 50 µm; (f, g, h) Different views of the male caudal region. Bar = 20 µm, Bar = 100 µm, Bar = 50 µm.

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Figure 2
Light microscopy of Globocephalus urosubulatus in ventral view of the caudal bursa showing the spicules (sp); pre-bursal papillae (pb) and papillae a = ventroventral (vr) and lateroventral rays (lr), b = anterolateral (ar) mediolateral (mr), posterolateral rays (pr), c = externodorsal rays (er) and d = dorsal trunk. Bar = 100µm.

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Table 1
Morphological and morphometric comparison of Globocephalus urosubulatus collected in Sus scrofa from State of Pará, Brazil.

Character	*Globocephalus urosubulatus*		*G. urosubulatus*		*G. urosubulatus*		*Globocephalus marsupialis*
Character	Male	Famele	Male	Female	Male	Famale	Male	Famale
Hosts Type locality	Sus scrofa Moju-Pará		Sus scrofa Rio de Janeiro		Sus scrofa Bulgaria		Metachirops opossum Petrópolis-Rio de Janeiro
Length (mm) ^a a Measurements in micrometers unless indicated and the parameter number of buds is given in amplitude;	4-5	6-8	3.16-5.32	5.02-6.53	3.5-5	4.5-8	4.4-6.18	10.47-11.44
Width	167-300	429-514	320-400	490-570	360-370	420-500	237-316	400-430
Buccal capsule ^{b, L}	125-150	167-227	119-127	192-220	140-200	140-200	104	129-143
Buccal capsule ^{b, W}	100-140	140-160	162-186	159-178	150-170	150-170	96	114-129
Nerve ring	317-367	387-500	290-460	380-460	380-520	380-520	360	429
Excretory pore	317-417	433-547	320-500	320-530	–	–	–	–
Deirids	370-533	433-547	–	–	430-610	430-610	–	–
Muscular esophagus ^a, ^b b Abbreviations: L: length, W: width; ^{, L}	0.487-0.540	0.593-0.687	0.550-0.710	0.680-0.75	0.560-0.690	0.560-0.690	0.789-0.800	1-1.3
Muscular esophagus ^{b, W}	93-133	147-173	110-170	110-160	120-150	120-150	–	–
Vulva (mm)	–	3-5	–	1.73-2.91	–	2.20-2.40^c c Distance between the vulva and the posterior body end.	–	4.4-5.7
Spicule	337-527	–	490-550	–	420-580	–	338-378	–
Gubernaculum	60-88	–	89-110	–	70-80	–	38-48	–
Tail	–	130-200	–	–	120-180	120-180	–	304-329
Mucron	Absent		–		–	40	–
Reference	From this study		Francis (1978)Francis M. Estudo da helmintofauna de Sus scrofa L., 1758 no Estado do Rio de Janeiro – Brasil [dissertation]. Rio de Janeiro: Universidade Federal Rural do Rio de Janeiro; 1978.		Nanev et al. (2007)Nanev V, Mutafova T, Todev I, Hrusanov D, Radev V. Morphological characteristics of Nematodes of the Globocephalus genus prevalent among wild boars from various regions of Bulgaria. Bulg J Vet Med 2007; 10(2): 103-111.		Freitas & Lent (1936)Freitas JFT, Lent H. Estudo sobre o genero Globocephalus Molin, 1861 (Nematoda: strongyloidea). Mem Inst Oswaldo Cruz 1936; 31(1): 69-79. http://dx.doi.org/10.1590/S0074-02761936000100004. http://dx.doi.org/10.1590/S0074-02761936...

[1] *Corresponding author: Elane Guerreiro Giese. E-mail: lheaufra@gmail.com

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Globocephalus urosubulatus (Alessandrini, 1909) (Nematoda: Ancylostomatidae) in Brazil: a morphological revisitation

Globocephalus urosubulatus (Alessandrini, 1909) (Nematoda: Ancylostomatidae) no Brasil: uma revisitação morfológica

Abstract