In this study the polymorphism and stability of isoenzymes and heat-resistant proteins in corn, bean, cotton, and soybean seeds with different levels of physiological quality were evaluated. The alcohol dehydrogenase, catalase, esterase and superoxide dismutase enzymes and simultaneous analysis were effective in identifying eight corn cultivars. It was observed for the bean cultivars that the peroxidase enzyme allowed differentiation of the Carioca bean cultivar from the others but, the peroxidase enzyme pattern varied in seeds with low-germination percentage. The varieties of cotton could not be differentiated by esterase enzyme, superoxide dismutase, diaphorase and malate dehydrogenase. The Conquista soybean cultivar was separated by superoxide dismutase and esterase enzyme systems and BRS-154 was separated by esterase. Heat-resistant protein patterns showed polymorphism and were stable for corn cultivar identification.
seeds; genetic purity; molecular marker; physiological quality
