Four Zebu × Holstein steers were fistulated in rumen, abomasums and esophagus, with 430 kg of live weight grazing in four paddocks of Brachiaria decumbens (.40 ha), feed supplements were used to evaluate intake, digestibility, pH and ammonia ruminal concentration, nitrogen in serum and urine and microbial yield. The animals received 4.0 kg/animal/day of supplement based on grounded corn grain, cottonseed meal, mineral mix (MM), ammonium sulfate and urea in four levels (0, 1.2, 2.4 and 3.6%) as fed basis. The intake of total (TDM) and pasture (DMP) dry matter, organic matter (OM), OM from pasture, crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), total carbohydrate (TO) and non-fiber carbohydrate (NFC) presented quadratic behavior. The maximum responses on the urea levels were: 2.0% (10.9 kg/day), 2.3% (7.1 kg/day), 2.2% (9.7 kg/day), 2.3% (6.3 kg/day), 1.9% (1.3 kg/day), 3.6% (0.4 kg/day), 1.9% (5.8 kg/day), 2.3% (8.2 kg/day) and 2.9% (4.1 kg/day), respectively, for TDM, DMP, OM, OMP, CP, EE, NFD, TC and NFC. The OM digestibility was affected with a linear behavior. No differences were observed for the pH values. However, ammonia concentration increased linearly as the urea level in the supplement increased. The urea level in supplement increased linearly the nitrogen in serum and urinary excretion of nitrogen from urea. No effect from the urea level on the microbial efficiency, expressed by different forms, was observed.
ammonia; digestibility; intake; non protein nitrogen; pH; protein supplementation
