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Effects of additive application on the microbial profile, physical parameters and aerobic stability of Tifton 85 (Cynodon ssp.) silages with different dry matter contents

The objectives of this study were to analyze the effects of wilting and bacterial-enzimatic additive (BEA) and buffered propionic acid (BPA) on the microbial profile, physical parameters and aerobic stability of Tifton 85 (Cynodon spp.) silages stored as squared bales (140 x 120 x 70 cm) wrapped with plastic bags. Treatments were based on five forage dry matter (DM) contents (250, 350, 450, 550 and 650 g/kg DM) without BEA. Other three forage dry matter contents (250, 450 and 650 g k/g DM) were associated with BEA while a wilted forage (650 g/kg DM) was sprayed with BPA. Silage samples were taken at ensiling time, and after 6 and 12 hours and 1, 2, 4, 8, 16, 32, 90 and 180 days of fermentation. The experimental design was completely randomized with three replications; with DM levels set as the main factor and fermentation time as a split-plot feature of the design. Silages containing higher DM contents (> 450 g/kg DM) showed lower bacterial counts, intermediary values of water activity (Aw) and silage N-NH3, and improved values of electrical conductivity (EC), pH and higher aerobic stability as compared to the wet silages. Results suggest a decrease in Aw followed by an inibition on microorganism growth and silage fermentation as the silage DM increased. The BEA had positive effect on the silage with dry-matter content of 450 g/kg, mainly on the 32nd day of storage, whereas BPA addition had no benefit.

bacterial-enzymatic additive; propionic acid; wilting


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