It was conducted an experiment using the semi-automatic in vitro cumulative gas production with the objective of evaluating the combination of energy and protein supplementations in diets based on low quality roughage, Tifton hay (Cynodon dactylon L.), with 7.69% of crude protein; 70.34% of neutral detergent fiber and 57.98% of organic matter in vitro digestibility. It was evaluated two sources of rumen degradable protein (urea or isolated soybean protein); four levels of ground corn grain (0, 20, 40 and 60%) and four supplementary levels of rumen degradable protein (0; 2.8; 5.6 and 8.4%) in complete random design in a 2 × 4 × 4 factorial arrangement. The organic matter in vitro digestibility increased linearly in response to grain corn inclusion with a greater slope by using isolated soybean protein. The partitioning factor was higher with isolated soybean protein and it was detected a linear increase in response to increasing rumen degradable protein with 20% of grain corn. The maximum gas production of the fraction of fast (A) and slow (D) degradation increased linearly in response to the grain corn inclusion, with isolated soybean protein, and it quadratically increased with urea. The A degradation rate was increased with inclusion of up to 20% of grain corn and the degradation rate of D was higher with urea. The A lag-time increased linearly with the inclusion of grain corn and in the D lag-time it was observed a decrease with up to 20% of grain corn unchanged at high levels. The supplementation with up to 60% of grain corn was beneficial to digestibility of diets with low quality roughage. The association between energy and protein supplementation was positive in some studied parameters. Urea gave better responses at moderate levels of ground grain corn.
corn; isolated soybean protein; protein sources; urea
