Four ruminally fistulated Holstein steers were fed four times a day (8 a.m., 11 a.m., 2 p.m. and 5 p.m.) with four diets with low and high protein content, with or without monensin, to verify the effect of monensin on feed intake and ruminal fermentation. Diets contained (dry matter basis): low (11.4 %) and high (16.5%) crude protein content, 65% of brachiaria hay (Brachiaria decumbens) and 35% of concentrate. Corn and urea- and corn and soybean meal- based diets were, respectively, the concentrates of low and high protein content. The level of ionophore was of 28 mg of monensin/kg of DM intake. Samples of ruminal fluid were collected from the animals two hours post-feeding. The experiment was analyzed as a Latin square with four treatments and four animals. High protein content -based diets increased ruminal concentration of butyric acid and ammonia. Feeding of diets with monensin, independently of the protein content, decreased dry matter intake, increased propionic acid concentration and decreased butyric acid, acetate:propionate ratio and specific activity of ammonia production . Monensin with low protein content-based diet also decreased acetic acid concentration and incerased ruminal pH and microbial protein synthesis. No significant differences on the concentration of ruminal ammonia for diet with monensin were observed.
ammonia; ionophore; ruminant; volatile fatty acid
