Hemócitos fagocitários em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Falleiros, Angela M.F; Gregório, Elisa A

doi:10.1590/S0101-81751995000400004

Resumo

The ultrastructural observation of the hemocytes of D. saccharalis larvae showed different hemocyte tvpes in various stage of interaction with microorganisms, which were interpreted as bacteria. The microorganisms were observed either free in the hemolymph or into the insect hemocytes. The bacteria were detected mostly into lhe granulocytes and plasmatocytes: the spherule cells and the oenocytoids also presented intracytoplasmic microorganisms, but less frequently. The fagocytic capacity of the different hemocyte tvpes is discussed.

Lepidoptera; Pyralidae; Diairaea saccharalis; hemocyte; phagocytosis; ultrastructure

Hemócitos fagocitários em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Phagocytic hemocytes in larval of Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Angela M.F. Falleiros^I; Elisa A. Gregório^II

^IDepartamento de Histologia. Centro de Ciências Biológicas. Universidade Estadual de Londrina, Caixa Postal 6001, 86051-970 Londrina, Paraná, Brasil

^IIDepartamento de Morfologia. Instituto de Biociências, Universidade Estadual Paulista, Caixa Postal 501, 18618-000 Botucatu, São Paulo, Brasil

ABSTRACT

The ultrastructural observation of the hemocytes of D. saccharalis larvae showed different hemocyte tvpes in various stage of interaction with microorganisms, which were interpreted as bacteria. The microorganisms were observed either free in the hemolymph or into the insect hemocytes. The bacteria were detected mostly into lhe granulocytes and plasmatocytes: the spherule cells and the oenocytoids also presented intracytoplasmic microorganisms, but less frequently. The fagocytic capacity of the different hemocyte tvpes is discussed.

Key words: Lepidoptera. Pyralidae. Diairaea saccharalis, hemocyte. phagocytosis. ultrastructure

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AGRADECIMENTOS. Os autores agradecem às Sras. Mana Euleda Lino Perez e Maria Helena Moreno, pelo processamento técnico do material Trabalho parcialmente financiado pelo CNPq (Proc. 140179/90-9)

Recebido em 10.VI.1995; aceito em 21.III.1996.

Anderson, R. & B.M. Chain. 1987. Macrophage functions in insects, p. 77-88. In: A.P. Gupta (Ed). Hemocytic and humoral immunity in arthropods. New York. Willey-Interscience, XII+533p.
Anggraeni, T. & N.A. Ratcliffe. 1991. Studies on cell-cell co-operation during phagocytosis by purified haemocyte populations of the moth, Galleria mellonella. Jour. Insect Physiol. 37 (6):453-460.
Baines, D.; T. DeSantis & R.G.H. Downer. 1992. Octopamine and 5-hydroxytryptamine enchance the phagocytic and nodule formation activities of cockroach (Periplaneta americana) haemocytes. Jour. Insect Physiol. 38 (11):905-914.
Beaulaton, J. & M. Monpeyssin. 1977. Ultrastructure et cytochimie des hémocytes d'Antheraea pernyi (Guér) (Lepidoptera, Attacidae). II Cellules à sphèrules et oenocytoides. Biol. Cell. 28:13-18.
Bombonato, M.T.S. 1994. Análise morfológica e quantitativa dos hemócitos de larvas de Diatraea saccharalis (Lepidoptera: Pyralidae) frente ao parasitóide Metagonistylum minense (Diptera: Tachinidae). Dissertação de Mestrado, não publicada, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, 90p.
Brookman, J.L.; N.A. Ratcliffe & A.F. Rowley. 1989. Studies on the activation of the prophenoloxidase system of insects by bacterial cell wall components. Insect Biochem. 19:47-57.
Chiang, A.S.; A.P. Gupta & S.S Han. 1988. Arthropod immune system: I. Comparative ligth and electron microscopic accounts of immunocytes and other hemocytes of Blattella germanica (Dictyoptera: Blattellidae). Jour. Morphol. 198:257-267.
Dunphy, G.B. & J.S. Chadwick. 1989. Effects of selected carbohydrate and the contribution of the prophenoloxidase cascade system to the adhesion of strains of Pseudomonas aeruginosa and Proteus mirabilis to hemocytes of nonimmune larval Galleria mellonella. Can. Jour. Microbiol. 35:524-527.
Ehlers, D.; B. Zosel; W. Mohrig; E. Kauschke & M. Ehlers. 1992. Comparison of in vivo and in vitro phagocytosis in Galleria mellonella L.. Parasitol. Res. 78:354-359.
Fenoglio, C.; P. Bernardini & M.V. Gervaso. 1993. Cytochemical characterization of the hemocytes of Leucophaea maderae (Dictyoptera: Blaberoidea). Jour. Morphol. 218:115-126.
Götz, P. 1986. Mechanisms of encapsulation in Diptera hosts. p. 1-19. In: A.M. Lackie (Ed.). Immune mechanisms in invertebrate vectors. Oxford, Claredon Press. V+285p.
Grégoire, C.H. & G. Goffinet. 1979. Controversies about the coagulocytes. p. 189-229. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, XVI+614p.
Gupta, A.P. 1985. Cellular elements in the hemolymph, p. 402-444. In: G.A. Kerkut & L.I. Gilgert (Eds). Comprehensive insect physiology biochemistry and pharmacology. Oxford. Pergamon Press, vol. 3, XVI+625p.
Hensley, S.D. & A.M. Hammond Jr. 1968. Laboratory techniques for rearing the sugarcane borer on an artificial diet. Jour. Econ. Entomol 61:1742-1743.
Hoffmann, J.A.; A. Porte & P. Joly. 1970. On the localization of phenoloxidase activity in coagulation of Locusta migratoria (L.) (Orthoptera). C.R. Hebd. Séances Acad. Sc. Paris. 270:629-631.
Hung, S.Y.; D.G. Boucias & A.J. Vey. 1993. Effect of Beauveria bassiana and Candida albicans on the cellular defense response of Spodoptera exigua. Jour. Invertebr. Pathol. 61:179-187.
Kaaya, G.P.; N.A. Ratcliffe & P. Alemu. 1986. Cellular and humoral defenses of Glossina: reactions against bacteria, trypanosomes and experimental implants. Jour. Med. Entomol. 23:30-43.
Krochinski, J. & L. Renwrantz. 1988. Determination of the pH values inside the digestive vacuoles of hemocytes from Mytilus edulis. Jour. Invertebr. Pathol 51:73-79.
Kurihara, Y.; T. Shimazu & H. Wago. 1992. Classification of hemocytes in the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) I. Phase microscopic study. Appl. Entomol. Zool. 27 (2):225-235.
Lackie, A.M. 1983. Effects of substratum wettability and charge on adhesion in vitro and encapsulation in vivo by insect haemocytes. Jour. Cell Sci. 63:181-190.
Leonard. C; K. Soderhall & N.A. Ratcliffe. 1985. Studies of pro-phenoloxidase and protease of Blaberus craniifer haemocytes. Insect Biochem. 15:803-810.
Neuwirth, M. 1974. Granular hemocytes, the main phagocytic blood cells in Calpodes ethlius (Lepidoptera, Hesperiidae). Can. Jour. Zool. 52:783-784.
Pendland, J.C.; M.A. Heat & D.G. Boucias. 1988. Functions of a galactosebinding lectin from Spodoptera exigua larval haemolymph: opsonization of blastospores from entomogeneous hyphomycetes. Jour. Insect Physiol. 34:533-540.
Rahmet-Alla, M. & A.F. Rowley. 1990. Studies on the cellular defense reactions of lhe madeira cockroach, Leucophaea maderae: in vitro phagocytosis of different strains of Bacillus cereus and their effect on hemocyte viability. Jour. Invertebr. Pathol. 55:350-356.
Ratcliffe, N.A. 1986. Insect cellular immunity and the recognition of foreignness, p. 21-43. In: A.M. Lackie (Ed.). Immune mechanisms in invertebrate veetors. Oxford, Claredon Press. V+285p.
Ratcliffe, N.A. & A.F. Rowley. 1975. Cellular defense reactions of insect hemocytes in vitro: phagocytosis in a new suspension culture system. Jour. Invertebr. Pathol. 26:225-233.
______. 1979. Role of hemocytes in defense against biological agents, p. 331-414. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press. XVI+614p.
Ratcliffe, N.A. & J.B. Walters. 1983. Studies on the in vivo cellular reactions of insects: clearance of pathogenic and non-pathogenic bacteria in Galleria mellonella larvae. Jour. Insect Physiol. 29:407-415.
Ratcliffe, N.A.; A.F. Rowley; S.W. Fitzgerald & C.P. Rhodes. 1985. Invertebrate immunity: basic concepts and recent advances. Int. Rev. Cytol. 97:183-349.
Rizki, T.M. & R.M. Rizki. 1984. The cellular defense of Drosophila melanogaster, p. 579-604. In: R.C. King & H. Akai (Eds). Insect Ultrastructure. New York, Plenum Press, vol. 2, XXV+624p.
Rowley, A.F. & N.A. Ratcliffe. 1976. The granular cells of Galleria mellonella during clotting phagocytic reactions in vitro. Tissue Cell. 8 (3):437-446.
Santos, R.R.; M.M.G. Este; E.S. Garcia & P. Azambuja. 1990. Binding sites for IgG-Fc in hemocyte adherent cells of hematophagous bugs (Rhodnius prolixas). Mem. Inst. Oswaldo Cruz 85 (4):469-470.
Yager, J.F. & H.H. Knight. 1983. Microscopic observation blood coagulation in several differents species of insects. Jour. Ent. Soc. Ainer. 26:591-602.

Datas de Publicação

Publicação nesta coleção
21 Jul 2009
Data do Fascículo
1995

Histórico

Aceito
21 Mar 1996
Recebido
10 Jun 1995

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Anderson, R. & B.M. Chain. 1987. Macrophage functions in insects, p. 77-88. In: A.P. Gupta (Ed). Hemocytic and humoral immunity in arthropods. New York. Willey-Interscience, XII+533p.

[2] Anggraeni, T. & N.A. Ratcliffe. 1991. Studies on cell-cell co-operation during phagocytosis by purified haemocyte populations of the moth, Galleria mellonella. Jour. Insect Physiol. 37 (6):453-460.

[3] Baines, D.; T. DeSantis & R.G.H. Downer. 1992. Octopamine and 5-hydroxytryptamine enchance the phagocytic and nodule formation activities of cockroach (Periplaneta americana) haemocytes. Jour. Insect Physiol. 38 (11):905-914.

[4] Beaulaton, J. & M. Monpeyssin. 1977. Ultrastructure et cytochimie des hémocytes d'Antheraea pernyi (Guér) (Lepidoptera, Attacidae). II Cellules à sphèrules et oenocytoides. Biol. Cell. 28:13-18.

[5] Bombonato, M.T.S. 1994. Análise morfológica e quantitativa dos hemócitos de larvas de Diatraea saccharalis (Lepidoptera: Pyralidae) frente ao parasitóide Metagonistylum minense (Diptera: Tachinidae). Dissertação de Mestrado, não publicada, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, 90p.

[6] Brookman, J.L.; N.A. Ratcliffe & A.F. Rowley. 1989. Studies on the activation of the prophenoloxidase system of insects by bacterial cell wall components. Insect Biochem. 19:47-57.

[7] Chiang, A.S.; A.P. Gupta & S.S Han. 1988. Arthropod immune system: I. Comparative ligth and electron microscopic accounts of immunocytes and other hemocytes of Blattella germanica (Dictyoptera: Blattellidae). Jour. Morphol. 198:257-267.

[8] Dunphy, G.B. & J.S. Chadwick. 1989. Effects of selected carbohydrate and the contribution of the prophenoloxidase cascade system to the adhesion of strains of Pseudomonas aeruginosa and Proteus mirabilis to hemocytes of nonimmune larval Galleria mellonella. Can. Jour. Microbiol. 35:524-527.

[9] Ehlers, D.; B. Zosel; W. Mohrig; E. Kauschke & M. Ehlers. 1992. Comparison of in vivo and in vitro phagocytosis in Galleria mellonella L.. Parasitol. Res. 78:354-359.

[10] Fenoglio, C.; P. Bernardini & M.V. Gervaso. 1993. Cytochemical characterization of the hemocytes of Leucophaea maderae (Dictyoptera: Blaberoidea). Jour. Morphol. 218:115-126.

[11] Götz, P. 1986. Mechanisms of encapsulation in Diptera hosts. p. 1-19. In: A.M. Lackie (Ed.). Immune mechanisms in invertebrate vectors. Oxford, Claredon Press. V+285p.

[12] Grégoire, C.H. & G. Goffinet. 1979. Controversies about the coagulocytes. p. 189-229. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, XVI+614p.

[13] Gupta, A.P. 1985. Cellular elements in the hemolymph, p. 402-444. In: G.A. Kerkut & L.I. Gilgert (Eds). Comprehensive insect physiology biochemistry and pharmacology. Oxford. Pergamon Press, vol. 3, XVI+625p.

[14] Hensley, S.D. & A.M. Hammond Jr. 1968. Laboratory techniques for rearing the sugarcane borer on an artificial diet. Jour. Econ. Entomol 61:1742-1743.

[15] Hoffmann, J.A.; A. Porte & P. Joly. 1970. On the localization of phenoloxidase activity in coagulation of Locusta migratoria (L.) (Orthoptera). C.R. Hebd. Séances Acad. Sc. Paris. 270:629-631.

[16] Hung, S.Y.; D.G. Boucias & A.J. Vey. 1993. Effect of Beauveria bassiana and Candida albicans on the cellular defense response of Spodoptera exigua. Jour. Invertebr. Pathol. 61:179-187.

[17] Kaaya, G.P.; N.A. Ratcliffe & P. Alemu. 1986. Cellular and humoral defenses of Glossina: reactions against bacteria, trypanosomes and experimental implants. Jour. Med. Entomol. 23:30-43.

[18] Krochinski, J. & L. Renwrantz. 1988. Determination of the pH values inside the digestive vacuoles of hemocytes from Mytilus edulis. Jour. Invertebr. Pathol 51:73-79.

[19] Kurihara, Y.; T. Shimazu & H. Wago. 1992. Classification of hemocytes in the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) I. Phase microscopic study. Appl. Entomol. Zool. 27 (2):225-235.

[20] Lackie, A.M. 1983. Effects of substratum wettability and charge on adhesion in vitro and encapsulation in vivo by insect haemocytes. Jour. Cell Sci. 63:181-190.

[21] Leonard. C; K. Soderhall & N.A. Ratcliffe. 1985. Studies of pro-phenoloxidase and protease of Blaberus craniifer haemocytes. Insect Biochem. 15:803-810.

[22] Neuwirth, M. 1974. Granular hemocytes, the main phagocytic blood cells in Calpodes ethlius (Lepidoptera, Hesperiidae). Can. Jour. Zool. 52:783-784.

[23] Pendland, J.C.; M.A. Heat & D.G. Boucias. 1988. Functions of a galactosebinding lectin from Spodoptera exigua larval haemolymph: opsonization of blastospores from entomogeneous hyphomycetes. Jour. Insect Physiol. 34:533-540.

[24] Rahmet-Alla, M. & A.F. Rowley. 1990. Studies on the cellular defense reactions of lhe madeira cockroach, Leucophaea maderae: in vitro phagocytosis of different strains of Bacillus cereus and their effect on hemocyte viability. Jour. Invertebr. Pathol. 55:350-356.

[25] Ratcliffe, N.A. 1986. Insect cellular immunity and the recognition of foreignness, p. 21-43. In: A.M. Lackie (Ed.). Immune mechanisms in invertebrate veetors. Oxford, Claredon Press. V+285p.

[26] Ratcliffe, N.A. & A.F. Rowley. 1975. Cellular defense reactions of insect hemocytes in vitro: phagocytosis in a new suspension culture system. Jour. Invertebr. Pathol. 26:225-233.

[27] ______. 1979. Role of hemocytes in defense against biological agents, p. 331-414. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press. XVI+614p.

[28] Ratcliffe, N.A. & J.B. Walters. 1983. Studies on the in vivo cellular reactions of insects: clearance of pathogenic and non-pathogenic bacteria in Galleria mellonella larvae. Jour. Insect Physiol. 29:407-415.

[29] Ratcliffe, N.A.; A.F. Rowley; S.W. Fitzgerald & C.P. Rhodes. 1985. Invertebrate immunity: basic concepts and recent advances. Int. Rev. Cytol. 97:183-349.

[30] Rizki, T.M. & R.M. Rizki. 1984. The cellular defense of Drosophila melanogaster, p. 579-604. In: R.C. King & H. Akai (Eds). Insect Ultrastructure. New York, Plenum Press, vol. 2, XXV+624p.

[31] Rowley, A.F. & N.A. Ratcliffe. 1976. The granular cells of Galleria mellonella during clotting phagocytic reactions in vitro. Tissue Cell. 8 (3):437-446.

[32] Santos, R.R.; M.M.G. Este; E.S. Garcia & P. Azambuja. 1990. Binding sites for IgG-Fc in hemocyte adherent cells of hematophagous bugs (Rhodnius prolixas). Mem. Inst. Oswaldo Cruz 85 (4):469-470.

[33] Yager, J.F. & H.H. Knight. 1983. Microscopic observation blood coagulation in several differents species of insects. Jour. Ent. Soc. Ainer. 26:591-602.

Brasil

Brasil

Hemócitos fagocitários em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Phagocytic hemocytes in larval of Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Resumo

Datas de Publicação

Histórico