Influência do jejum severo sobre o conteúdo de proteínas totais e de amônio na hemolinfa de Bradybaena similaris (Fèrussac) (Mollusca, Gastropoda, Xanthonychidae)

Lira, Claudia R.S. de; Gomes, Edna M.; Chagas, Generoso M.; Pinheiro, Jairo

doi:10.1590/S0101-81752000000400003

Resumo

The total protein and ammonia contents in the hemolymph of Bradybaena similaris (Fèrussac, 1821) during the starvation were determined from August, 1996 to March, 1998. The results revealed a significant variation in the total protein content throughout the period of starvation analized, there being a negative relation between the total protein content and the time of starvation. But the same result was not observed in the ammonia content. After 30 days of starvation there was a reduction of 2,8% of the content of ammonia in the hemolymph. A negative relation between the ammonia content and the time of starvation was observed, but this relation was not significative. It is probable that the degradation of the proteins don't lead to an increase of the ammonia in the hemolymph of the snail. It indicates that the increase on the nitrogenous products of degradation, caused by the degradation of the proteins, is detoxified as urea or uric acid.

Bradybaena similaris; ammonium; nitrogenous products; starvation; total proteins

Influência do jejum severo sobre o conteúdo de proteínas totais e de amônio na hemolinfa de Bradybaena similaris (Fèrussac) (Mollusca, Gastropoda, Xanthonychidae)

Influence of the starvation on the total proteins and ammonia contents in the hemolymph of Bradybaena similaris (Fèrussac) (Gastropoda)

Claudia R.S. de Lira^I; Edna M. Gomes^II; Generoso M. Chagas^II; Jairo Pinheiro^II

^ICurso de Pós-graduação em Ciências Biológicas, Universidade Federal Rural do Rio de Janeiro

^IIDepartamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio Janeiro. BR 465, Km 7, 23890-000 Seropédica, Rio de Janeiro, Brasil. E-mail: jpinheirosilva@bol.com.br ou jps@ufrrj.br

ABSTRACT

The total protein and ammonia contents in the hemolymph of Bradybaena similaris (Fèrussac, 1821) during the starvation were determined from August, 1996 to March, 1998. The results revealed a significant variation in the total protein content throughout the period of starvation analized, there being a negative relation between the total protein content and the time of starvation. But the same result was not observed in the ammonia content. After 30 days of starvation there was a reduction of 2,8% of the content of ammonia in the hemolymph. A negative relation between the ammonia content and the time of starvation was observed, but this relation was not significative. It is probable that the degradation of the proteins don't lead to an increase of the ammonia in the hemolymph of the snail. It indicates that the increase on the nitrogenous products of degradation, caused by the degradation of the proteins, is detoxified as urea or uric acid.

Key words:Bradybaena similaris, ammonium, nitrogenous products, starvation, total proteins

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AGRADECIMENTO. À Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), pelo suporte financeiro (Processo E-26/150.122/99).

Recebido em 09.IX.1999; aceito em 26.IX.2000.

Amato, S.B. & J.C.B. Bezerra. 1989. Concurrent infection of Postharmostomum gallinum (Digenea, Brachylaimidae) and Eurytrema coelomaticum (Digenea, Dicrocoeliidae) in Bradybaena similaris (Stylommatophora, Xanthonychidae). Mem. Inst. Oswaldo Cruz 87(2):309-311.
Barros-Araújo, J.L. de. 1989. Moluscos de importância econômica no Brasil. I. Xanthonychidae: Bradybaena similaris (Fèrussac, 1821) (Mollusca, Gastropoda, Pulmonata, Stylommatophora). Revta bras. Zool. 6:583-592.
Becker, W. 1980. Metabolic interrelationship of parasitic trematodes and molluscs, especially Schistosoma mansoni in Biomphalaria glabrata. Z. Parasitenkd. 63:101-111.
______. 1983. Purine metabolism in Biomphalaria glabrata under starvation and infection with Schistosoma mansoni. Comp. Biochem. Physiol. 76B:215-219.
Becker, W. & H. Schmale. 1975. The nitrogenous products of degradation ammonia, urea and uric acid- in the hemolymph of the snail Biomphalaria glabrata. Comp. Biochem. Physiol. 51A:407-411.
______. 1978. The ammonia and urea excretion of Biomphalaria glabrata under different physiological conditions: starvation, infection with Schistosoma mansoni, dry keeping. Comp. Biochem. Physiol. 59B:75-79.
Bishop, S.H.; L.L. Ellis & S.M. Burcham. 1983. Amino Acid Metabolism in Molluscs. p.243-327. In: The Mollusca. Vol. 1. Metabolic Biochemistry and Molecular Biomechanics. New York, Academic Press Inc., 510p.
Felker, P. 1977. Microdetermination of nitrogen in seed protein extracts. 1977. Anal. Chem. 49:1080.
Graeff-Teixeira, C.; S.C. Thiengo; J.W. Thomé; A.B. Medeiros; L. Camillo-Coura & A.A. Agostini. 1993. On the diversity of mollusc intermediate hosts of Angiostrogylus costaricensis (Morera & Céspedes, 1971) in southern Brazil. Mem. Inst. Oswaldo Cruz 88:487-489.
Horne, F.R. 1973. Urea metabolism in an estivating terrestrial snail Bulimulus dealbatus. Amer. Jour. Physiol. 224(4):781-787.
Jezewska, M.M.; B. Gorzkowski & J. Heller. 1963. Nitrogen compounds in Helix pomatia excretion. Acta biochimica Pol. 10:55-65.
Joosse, J. & R. van Elk. 1986. Trichobilharzia ocellata: physiological characterization of giant growth, glycogen depletion, and absence of reproductive activity in the intermediate snail host, Lymnaea stagnalis. Exptl Parasitol. 62:1-13.
Jorge, F.B. de; J.A. Petersen & A.S.F. Ditadi. 1965. Variations in nitrogenous compounds in the urine of Strophocheilus (Pulmonata, Mollusca) with different diets. Experientia 25:614-615.
Mitchell, H.T. 1972. Microdetermination of nitrogen in plant tissues. Jour. Ass. agric. Chem. 55:1-3.
Pinheiro, J. 1996. Influence of starvation on the glycogen and galactogen contents in the snail Bradybaena similaris (Férussac, 1821) (Mollusca, Gastropoda). Arq. Biol. Tecnol., Curitiba, 39(2):349-357.
Schmale, H. & W. Becker. 1975. Die Ammonium- und Harnstoffkonzentrationin der Hämolinphe von Biomphalaria glabrata unter den Einfluβ einer Infecktion mit Schsitosoma mansoni. Z. parasitenkd. 45:237-241.
Souza, R.M. de; E.M. Gomes; G.M. Chagas & J. Pinheiro. (in press). The influence of starvation and Eurytrema coelomaticum infection on the nitrogenous products of degradation in the hemolymph of Bradybaena similaris. Arq. Biol. Tecnol., Curitiba.
Speeg Jr., K.V. & J.W. Campbell. 1968. Arginine and urea metabolism in terrestrial snails. Amer. Jour. Physiol. 216:1003-1011.
Tramell, P.R. & J.W. Campbell. 1970. Nitrogenous excretory products of the giant south american land snail, Strophocheilus oblongus. Comp. Biochem. Physiol. 32:569-571.
Villela, G.G.; M. Bacila & H. Tastaldi. 1977. Técnicas e Experimentos de Bioquímica. Rio de Janeiro, Editora Guanabara Koogan S.A., 552p.

Datas de Publicação

Publicação nesta coleção
30 Abr 2009
Data do Fascículo
Dez 2000

Histórico

Recebido
09 Set 1999
Aceito
26 Set 2000

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Amato, S.B. & J.C.B. Bezerra. 1989. Concurrent infection of Postharmostomum gallinum (Digenea, Brachylaimidae) and Eurytrema coelomaticum (Digenea, Dicrocoeliidae) in Bradybaena similaris (Stylommatophora, Xanthonychidae). Mem. Inst. Oswaldo Cruz 87(2):309-311.

[2] Barros-Araújo, J.L. de. 1989. Moluscos de importância econômica no Brasil. I. Xanthonychidae: Bradybaena similaris (Fèrussac, 1821) (Mollusca, Gastropoda, Pulmonata, Stylommatophora). Revta bras. Zool. 6:583-592.

[3] Becker, W. 1980. Metabolic interrelationship of parasitic trematodes and molluscs, especially Schistosoma mansoni in Biomphalaria glabrata. Z. Parasitenkd. 63:101-111.

[4] ______. 1983. Purine metabolism in Biomphalaria glabrata under starvation and infection with Schistosoma mansoni. Comp. Biochem. Physiol. 76B:215-219.

[5] Becker, W. & H. Schmale. 1975. The nitrogenous products of degradation ammonia, urea and uric acid- in the hemolymph of the snail Biomphalaria glabrata. Comp. Biochem. Physiol. 51A:407-411.

[6] ______. 1978. The ammonia and urea excretion of Biomphalaria glabrata under different physiological conditions: starvation, infection with Schistosoma mansoni, dry keeping. Comp. Biochem. Physiol. 59B:75-79.

[7] Bishop, S.H.; L.L. Ellis & S.M. Burcham. 1983. Amino Acid Metabolism in Molluscs. p.243-327. In: The Mollusca. Vol. 1. Metabolic Biochemistry and Molecular Biomechanics. New York, Academic Press Inc., 510p.

[8] Felker, P. 1977. Microdetermination of nitrogen in seed protein extracts. 1977. Anal. Chem. 49:1080.

[9] Graeff-Teixeira, C.; S.C. Thiengo; J.W. Thomé; A.B. Medeiros; L. Camillo-Coura & A.A. Agostini. 1993. On the diversity of mollusc intermediate hosts of Angiostrogylus costaricensis (Morera & Céspedes, 1971) in southern Brazil. Mem. Inst. Oswaldo Cruz 88:487-489.

[10] Horne, F.R. 1973. Urea metabolism in an estivating terrestrial snail Bulimulus dealbatus. Amer. Jour. Physiol. 224(4):781-787.

[11] Jezewska, M.M.; B. Gorzkowski & J. Heller. 1963. Nitrogen compounds in Helix pomatia excretion. Acta biochimica Pol. 10:55-65.

[12] Joosse, J. & R. van Elk. 1986. Trichobilharzia ocellata: physiological characterization of giant growth, glycogen depletion, and absence of reproductive activity in the intermediate snail host, Lymnaea stagnalis. Exptl Parasitol. 62:1-13.

[13] Jorge, F.B. de; J.A. Petersen & A.S.F. Ditadi. 1965. Variations in nitrogenous compounds in the urine of Strophocheilus (Pulmonata, Mollusca) with different diets. Experientia 25:614-615.

[14] Mitchell, H.T. 1972. Microdetermination of nitrogen in plant tissues. Jour. Ass. agric. Chem. 55:1-3.

[15] Pinheiro, J. 1996. Influence of starvation on the glycogen and galactogen contents in the snail Bradybaena similaris (Férussac, 1821) (Mollusca, Gastropoda). Arq. Biol. Tecnol., Curitiba, 39(2):349-357.

[16] Schmale, H. & W. Becker. 1975. Die Ammonium- und Harnstoffkonzentrationin der Hämolinphe von Biomphalaria glabrata unter den Einfluβ einer Infecktion mit Schsitosoma mansoni. Z. parasitenkd. 45:237-241.

[17] Souza, R.M. de; E.M. Gomes; G.M. Chagas & J. Pinheiro. (in press). The influence of starvation and Eurytrema coelomaticum infection on the nitrogenous products of degradation in the hemolymph of Bradybaena similaris. Arq. Biol. Tecnol., Curitiba.

[18] Speeg Jr., K.V. & J.W. Campbell. 1968. Arginine and urea metabolism in terrestrial snails. Amer. Jour. Physiol. 216:1003-1011.

[19] Tramell, P.R. & J.W. Campbell. 1970. Nitrogenous excretory products of the giant south american land snail, Strophocheilus oblongus. Comp. Biochem. Physiol. 32:569-571.

[20] Villela, G.G.; M. Bacila & H. Tastaldi. 1977. Técnicas e Experimentos de Bioquímica. Rio de Janeiro, Editora Guanabara Koogan S.A., 552p.

Brasil

Brasil

Influência do jejum severo sobre o conteúdo de proteínas totais e de amônio na hemolinfa de Bradybaena similaris (Fèrussac) (Mollusca, Gastropoda, Xanthonychidae)

Influence of the starvation on the total proteins and ammonia contents in the hemolymph of Bradybaena similaris (Fèrussac) (Gastropoda)

Resumo

Datas de Publicação

Histórico