Aspectos morfológicos de ciliados de rúmen (Entodiniomorphida, Ophryoscolecidae) revelados pelas técnicas do Protargol e da reação de Feulgen

D'Agosto, Marta; Santa-Rosa, Milden Rodrigues de

doi:10.1590/S0101-81751994000200005

Resumo

The resulls of the protargol's technique and Feulgen's reactions on ciliates of the genus Entodinium Stetn. 1859, Diplodinium Schuberg. 1888. Eodinium Kofoid & MacLennan. 1932, Eudiplodinium Dogiel. 1927 and Ostracodinium Dogiel. 1927 are presented. The ciliates were obtained from samples of bovine's ruminal content.

Entodiniomorphida; Ophryoscolecidae; rumen ciliate; silver impregnation; Feulgen's reaction

Aspectos morfológicos de ciliados de rúmen (Entodiniomorphida, Ophryoscolecidae) revelados pelas técnicas do Protargol e da reação de Feulgen

Morphological aspects of rumen ciliate (Entodiniomorphida, Ophryoscolecidae) demonstred by Protargol and Feullgen's reation techniques

Marta D'Agosto^I; Milden Rodrigues de Santa-Rosa^II

^IDepartamento de Zooloyia, Universidade Federal de Juiz de Fora, 36036-330 Juiz de Fora, Minas Gerais, Brasil

^IIInstituto de Microbiologia, Universidade Federal do Rio de Janeiro. 21944-970 Rio de Janeiro, Rio de Janeiro, Brasil

ABSTRACT

The resulls of the protargol's technique and Feulgen's reactions on ciliates of the genus Entodinium Stetn. 1859, Diplodinium Schuberg. 1888. Eodinium Kofoid & MacLennan. 1932, Eudiplodinium Dogiel. 1927 and Ostracodinium Dogiel. 1927 are presented. The ciliates were obtained from samples of bovine's ruminal content.

Key WORDS: Entodiniomorphida, Ophryoscolecidae, rumen ciliate, silver impregnation, Feulgen's reaction

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Recebido em 15.III.1993; aceito em 22.IV.1994.

CHARDEZ, D. 1983. Sur la variabilité de deux espèces d'Ophryoscolecidae (Ciliophora, Entodiniomorphida). Bull. Rech. Agron. Gembloux 18: 257-264.
Chatton, E. & A. Lwoff. 1930. Impregnation, par diffusion argentique de l'infraciliatura des ciliés marins et d'eau douce, après fixation cytologique et sans dessication. CR. Soc. Biol. 104: 834-836.
Clarke, R.T.J. 1964. Ciliates of the rumen of domestic cattle (Bos taurus L.). N.Z.J. Agric. Res. 7: 248-257.
Das-Gupta, M. 1935. Preliminary observations on the protozoan fauna of the rumen of the indian goat, Capra hircus Linn. Arch. Protist. 85: 153-172.
Dehority, B.A. 1974. Rumen ciliate fauna of alaskan moose (Alces americana), musk-ox (Ovibos moschattus) and dall mountain sheep (Ovis dalli). J. Protozool. 21: 26-32.
Fernandez-GaliaNo, D. 1958. La infraciliación en Polyplastron multivesiculatum y su gênesis durante la división del ciliado. Bol. R. Soc. Esp. Hist. Nat. (Secc. Biol.) 56: 89-108.
Frankel, J. & K. Heckmann. 1968. A simplified Chatton-Lwoff silver impregnation procedure for use in experimental studies with ciliates. Trans. Amer. Microsc. Soc. 87: 317-321.
Feulgen, R. 1926. Die nuclealfarburg abderharden's. 1926. Haudb. biol. Arbests, meth Abt, 5: 1055.
Foissner, W. 1991. Basic ligth and scanning electron microscopic methods for taxonomic studies of ciliate protozoa. Europ. J. Protistol. 27: 313-330.
Gruby, D. & O. Delafond. 1843. Recherches sur des animalcules se développant en grand nombre dans l'estomac et dans les intestins, pendant la digestion des animaux herbivores et carnivores. Compt. Rend. Acad. Sci. 17:1304-1308.
Imai, S. 1988. Ciliate protozoa in the rumen of kenyan zebu cattle, Bos taurus indicus, with the description of four new species. J. Protozool. 35: 130-136.
Klein, B. M. 1958. The "dry" silver method and its proper use. J. Protozool. 5: 99-103.
Kofoid, CA. & J.F. Christenson. 1934. Ciliates from Bos gaurus. Univ. Calif. Publ. Zool. 39: 341-391.
Kofoid, CA. & R.F. Maclennan. 1930. Ciliates from Bos indicus Linn. I. The genus Entodinium Stein. Univ. Calif. Publ. Zool. 33: 471-544.
______. 1932. Ciliates from Bos indicus Linn. II. A revision of Diplodinium Schuberg. Univ. Calif. Publ. Zool. 37: 53-152.
Noirot-Timothée, C. 1960. Étude d'une famille de ciliés: les Ophryoseoleeidae. Strutures et ultrastructures. Ann. Sci. Nat. Zool. 2: 527-718.
Ogimoto, K. & S. Imai. 1981. Atlas of rumen biology. Tokio, Scientific Societies Press, VIII+ 231p.
Tuffrau, M. 1964. Quelques variantes techniques de l'impregnation des ciliés par le Protéinate d'argent. Arch. Zool. Exp. 104: 186-190.
______. 1967. Perfectionnement et pratique de la technique d'impregnation au Protargol des infusoires eiliés. Protistologica 3: 91-98.
Van Hoven, W. 1975. Rumen ciliates of the tsessebe (Damaliscus lunatus lunatus) in South África. J. Protozool. 22: 457-462.
______. 1983. Rumen ciliates with description of two new speeies from three african reedbuck species. J. Protozool. 30: 688-691.
Wertheim. P. 1935. A new ciliate, Entodinium bovis n. sp., from the stomach of Bos taurus L. with the revision of Entodinium exiguum, E. nanellum, E. siniplex, E. dubardi dubardi and E. parvum. Parasitology 27: 226-230.
Zielyk, M.W. 1961. Ophryoscolecid fauna from the stomach of the white-tailed deer (Odocoileus virginianus borealis), and observations on the division of Entodinium dubardi Buisson, 1923 (Ciliata Entodiniomorpha). J. Protozool. 8:33-41.

Datas de Publicação

Publicação nesta coleção
22 Jul 2009
Data do Fascículo
1994

Histórico

Recebido
15 Mar 1993
Aceito
22 Abr 1994

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] CHARDEZ, D. 1983. Sur la variabilité de deux espèces d'Ophryoscolecidae (Ciliophora, Entodiniomorphida). Bull. Rech. Agron. Gembloux 18: 257-264.

[2] Chatton, E. & A. Lwoff. 1930. Impregnation, par diffusion argentique de l'infraciliatura des ciliés marins et d'eau douce, après fixation cytologique et sans dessication. CR. Soc. Biol. 104: 834-836.

[3] Clarke, R.T.J. 1964. Ciliates of the rumen of domestic cattle (Bos taurus L.). N.Z.J. Agric. Res. 7: 248-257.

[4] Das-Gupta, M. 1935. Preliminary observations on the protozoan fauna of the rumen of the indian goat, Capra hircus Linn. Arch. Protist. 85: 153-172.

[5] Dehority, B.A. 1974. Rumen ciliate fauna of alaskan moose (Alces americana), musk-ox (Ovibos moschattus) and dall mountain sheep (Ovis dalli). J. Protozool. 21: 26-32.

[6] Fernandez-GaliaNo, D. 1958. La infraciliación en Polyplastron multivesiculatum y su gênesis durante la división del ciliado. Bol. R. Soc. Esp. Hist. Nat. (Secc. Biol.) 56: 89-108.

[7] Frankel, J. & K. Heckmann. 1968. A simplified Chatton-Lwoff silver impregnation procedure for use in experimental studies with ciliates. Trans. Amer. Microsc. Soc. 87: 317-321.

[8] Feulgen, R. 1926. Die nuclealfarburg abderharden's. 1926. Haudb. biol. Arbests, meth Abt, 5: 1055.

[9] Foissner, W. 1991. Basic ligth and scanning electron microscopic methods for taxonomic studies of ciliate protozoa. Europ. J. Protistol. 27: 313-330.

[10] Gruby, D. & O. Delafond. 1843. Recherches sur des animalcules se développant en grand nombre dans l'estomac et dans les intestins, pendant la digestion des animaux herbivores et carnivores. Compt. Rend. Acad. Sci. 17:1304-1308.

[11] Imai, S. 1988. Ciliate protozoa in the rumen of kenyan zebu cattle, Bos taurus indicus, with the description of four new species. J. Protozool. 35: 130-136.

[12] Klein, B. M. 1958. The "dry" silver method and its proper use. J. Protozool. 5: 99-103.

[13] Kofoid, CA. & J.F. Christenson. 1934. Ciliates from Bos gaurus. Univ. Calif. Publ. Zool. 39: 341-391.

[14] Kofoid, CA. & R.F. Maclennan. 1930. Ciliates from Bos indicus Linn. I. The genus Entodinium Stein. Univ. Calif. Publ. Zool. 33: 471-544.

[15] ______. 1932. Ciliates from Bos indicus Linn. II. A revision of Diplodinium Schuberg. Univ. Calif. Publ. Zool. 37: 53-152.

[16] Noirot-Timothée, C. 1960. Étude d'une famille de ciliés: les Ophryoseoleeidae. Strutures et ultrastructures. Ann. Sci. Nat. Zool. 2: 527-718.

[17] Ogimoto, K. & S. Imai. 1981. Atlas of rumen biology. Tokio, Scientific Societies Press, VIII+ 231p.

[18] Tuffrau, M. 1964. Quelques variantes techniques de l'impregnation des ciliés par le Protéinate d'argent. Arch. Zool. Exp. 104: 186-190.

[19] ______. 1967. Perfectionnement et pratique de la technique d'impregnation au Protargol des infusoires eiliés. Protistologica 3: 91-98.

[20] Van Hoven, W. 1975. Rumen ciliates of the tsessebe (Damaliscus lunatus lunatus) in South África. J. Protozool. 22: 457-462.

[21] ______. 1983. Rumen ciliates with description of two new speeies from three african reedbuck species. J. Protozool. 30: 688-691.

[22] Wertheim. P. 1935. A new ciliate, Entodinium bovis n. sp., from the stomach of Bos taurus L. with the revision of Entodinium exiguum, E. nanellum, E. siniplex, E. dubardi dubardi and E. parvum. Parasitology 27: 226-230.

[23] Zielyk, M.W. 1961. Ophryoscolecid fauna from the stomach of the white-tailed deer (Odocoileus virginianus borealis), and observations on the division of Entodinium dubardi Buisson, 1923 (Ciliata Entodiniomorpha). J. Protozool. 8:33-41.