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Hematologia comparada entre diplóides e triplóides de truta arco-íris, Oncorhynchus mykiss walbaum (Pisces, Salmonidae)

Comparated hematology between diploids and triploids of rainbow trout, Oncorhynchus mykiss waibaum (Pisces, Salmonidae)

Resumo

This Study was carried out at the Salmoniculture Experimental Station "Dr. Ascanio de Faria", in Campos do Jordão, São Paulo. Brazil. The aim was to compared the hematology of triploid and diploid rainbow trout, Oncorhynchus mykiss Walbaum, 1792, by sex and gonadal maturation stages. Blood was obtained from 144 individuais for determinati-ons of: haemoglobin rate (Hb), haematocrit (Ht), erythrocyte count (Er), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MHC), mean corpuscular hemoglobin concentration (MCHC), and differential leucocyte counts. The ploid was determinated by measuring the erythrocyte major axis, in fresh and stainncd smears. Triploid rainbow trout had a large eiythrocyte diameter, mean erythrocyte volume and mean haemoglobin concentration, but lower erythrocyte count than diploid. Lymphocytes was the most frequent cell in peripherical blood.There was no dilTerence between males and females for ali parameters here analysed. The haematological analyses showcd signiffleant increase with the dcvelopment of gonadal maturation stages.

Oncorhynchus mykiss; diploid; triploid; hematolology; rainbow trout


Oncorhynchus mykiss; diploid; triploid; hematolology; rainbow trout

Hematologia comparada entre diplóides e triplóides de truta arco-íris, Oncorhynchus mykiss walbaum (Pisces, Salmonidae)

Comparated hematology between diploids and triploids of rainbow trout, Oncorhynchus mykiss waibaum (Pisces, Salmonidae)

Maria José Tavares Ranzani-PaivaI,III; Yara Aiko TabataII; Augusta Cocuzza das EirasI

IInstituto de Pesca, Divisão de Pesca Interior. Avenida Francisco Matarazzo455, 05031-900 São Paulo, São Paulo, Brasil. E-mail: ipesca@eu.ansp.br

IIInstituto de Pesca, Estação Experimental de Salmonicultura Dr. Ascânio de Faria. Caixa Postal 361, 12470-000 Campos de Jordão, São Paulo, Brasil

IIIBolsista do CNPq

ABSTRACT

This Study was carried out at the Salmoniculture Experimental Station "Dr. Ascanio de Faria", in Campos do Jordão, São Paulo. Brazil. The aim was to compared the hematology of triploid and diploid rainbow trout, Oncorhynchus mykiss Walbaum, 1792, by sex and gonadal maturation stages. Blood was obtained from 144 individuais for determinati-ons of: haemoglobin rate (Hb), haematocrit (Ht), erythrocyte count (Er), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MHC), mean corpuscular hemoglobin concentration (MCHC), and differential leucocyte counts. The ploid was determinated by measuring the erythrocyte major axis, in fresh and stainncd smears. Triploid rainbow trout had a large eiythrocyte diameter, mean erythrocyte volume and mean haemoglobin concentration, but lower erythrocyte count than diploid. Lymphocytes was the most frequent cell in peripherical blood.There was no dilTerence between males and females for ali parameters here analysed. The haematological analyses showcd signiffleant increase with the dcvelopment of gonadal maturation stages.

Key words:Oncorhynchus mykiss, diploid, triploid, hematolology, rainbow trout.

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AGRADECIMENTOS. As autoras agradecem ao PqC Benedicto do Espírito-Santo de Campos, do Instituto de Zootecnia de São Paulo, pelas análises estatísticas e ao Biólogo Clóvis Guimarães Salinos pelo auxílio nas coletas e nas análises dos resultados.

REFERÊNCIAS BIBLIOGRÁFICAS

BECK, M.L. & C.J. BIGGERS. 1983. Erythrocyte measurements of diploid and triploid Ctenophryngodon idella x Hypophthalmichthys nobilis hibrids. Jour. Fish Biol. 22: 497-502.

BENFEY, T.J. & A.M. Sutterlin. 1984a. Oxygen utilization by triploidy landlocked Atlantic salmon (Salmo salar L.). Aquaculture 42: 69-73.

______. 1984b. The haematology of triploid landlocked Atlantic salmon, Salmo salar L. Jour. Fish Biol. 24 (3): 333-338.

BENFEY, T.J.; A.M. SUTTERLIN; R.J. & THOMPSON. 1984. Use of erythrocyte measurements to identify triploid salmonids. Can. Jour. Fish. Aquat. Sci. 41 (6): 980-984.

BYE, V.J. & R.F. LINCOLN. 1986. Comercial methods for the control of sexual maturation in rainbow trout (Salmo gairdneri R.). Aquaculture 57: 299-309.

CHERPAS, N.B. 1966. Natural triploiy in females of the unisexual form of goldfish (Carassius auratus gibelio Bloch). Soviet Genet. 2 (5): 9-13.

CHOURROUT, D. 1980. Thermal induetion of diploid gynogenesis and triploidy in the eegs of the rainbow trout (Salmo gairdneri Richardson). Reprod. Nutr. Dévelop. 20 (3): 727-733.

CIMINO, M.C. 1973. Karyotypes and erythrocyte size of some diploid and triploid fishes of the genus Poeciliopsis. Jour. Fish. Res. Bd. Can. 30: 1736-1737.

COLLIER, H.B. 1944. The standardization of blood haemoglobin determinations. Can. Med. Ass. Jour. 50: 550-552.

COSTA NETO, P.L. 1977. Estatística. São Paulo, Edgard Blüncher, 246p.

Donaldson, E.M. & G.A. Hunter. 1982. Sex control in fish with particular reference to salmonids. Can. Jour. Fish. Aquat. Sci 39: 99-110.

GOLDENFARB, P.B.; F.P. BOWYER; E. HALL & E. BROSIUS. 1971. Reproducibility in the hematology laboratory: The micro-hematocrit determinations. Amer. Jour. clin. Pathol. 56 (1): 35-39.

GRAHAM, M.S.; G.L. FLETCHER & T.J. BENFEY. 1985. Effect of triploidy on blood oxygen eontent of Atlantic salmon. Aquaculture 50: 133-139.

HARDIG, J. & L.B. HOUGLUND. 1984. Seasonal variation in blood components of reared Baltic salmon, Salmo salar L. Jour. Fish Biol. 24 (5): 565-579.

HARVEY, W.R. 1987. User's guide for LSMLMW: Mixed model-version. Ohio State University, 59p.

IHSSEN, P.E.; L.R. MCKAY; I. MCMILLAN & R.B. PHILLIPS. 1990. Ploidy manipulation and gynogenesis in fishes: citogenetic and fisheries applications. Trans. Amer. Fish. Soc. 119: 698-717.

LINCOLN, R.S & A.P. SCOTT. 1984. Sexual maturation in triploid rainbow trout, Salmo gairdneri Richardson. Jour. Fish Biol. 25: 385-392.

MCCARTHY, D.H.; J.P. STEVENSON & M.S. ROBERTS. 1973. Some blood parameters of the rainbow trout (Salmo gairdneri Richardson) I. The Kamloops variety. Jour. Fish Biol. 5: 1-8.

MEADE, T.L. & S.J. PERRONE. 1980. Selective haematological parameters in steelhead trout, Salmo gairdneri Richardson. Jour. Fish Biol. 17 (1): 9-12.

PAIVA, M.J.T.R. 1991. Características sangüíneas da pirapitinga do sul, Brycon sp., sob condições experimentais de criação intensiva. Braz. Jour. Vet. Res. Anim. Scie. 28 (2): 141-153.

PAIVA, P.; H.M. GODINHO; C.S.R. MAINARDES-PINTO; Y.A. TABATA & R.G. LEITE. 1985. Comportamento reprodutivo de truta arco-íris, Salmo irideus Gibbons (Osteichithyes, Salmonidae), em cultivo intensivo. Bol. Inst. Pesca 12 (4):61-70.

PALÁCIOS, L.; G. VISCOR; J. PALOMEQUE. 1987. Comparative study of two methods to determine erythrocyte dimensions in vertebrates. An. Fac. Vet. León 33: 53-60.

PICKERING, A.D. 1986. Changes in blood cell composition of the brown trout, Salmo trutta L., during the spawning season. Jour. Fish Biol. 29: 335-347.

PURDOM, C. E. 1983. Genetic engineering by the manipulation of chromosomes. Aquaculture 33: 287-300.

RANZANI-PAIVA, M.J.T. 1995. Características hematológicas da tainha, Mugil platamis Günther, 1880 (Osteichthyes, Mugilidae) da região estuarino-lagunar de Cananéia, SP (Lat. 25º00"S - Long. 47º55'W). Bol. Inst. Pesca 2 (1): 1-22.

RANZANI-PAIVA, M.J.T. & H.M. GODINHO. 1985. Estudos hematológicos em curimbatá, Prochilodas scrofa Steindachner, 1881 (Osteichthyes, Cyprinifor-mes, Prochilodontidae). Série vermelha. Bol. Inst. Pesca 12 (2): 25-35.

ROSEFIELD, G. 1947. Corante pancrômico para hematologia e citologia clínica. Nova combinação dos componentes do May-Grünwald e do Giemsa num só corante de emprego rápido. Mem. Inst. Butantan 20: 329-334

SEZAKI, K.; S. WATANABE & K. HASHIMOTO. 1988. Haematological parameters and erythocyte enzyme activities assoeiatcd with increase in ploidy status of the spinous loach, Cobitis biwae Jordan and Snyder. Jour. Fish Biol. 32 (1): 149-150.

SMALL, S.A & T.J. BENFEY. 1987. Cell size in triploid salmon. Jour. Exp. Zool. 241: 339-342.

SWARUP, H. 1959. Effect of triploidy on the blood size, general organization and cellular strueture in Gasterosteus aculeatus (L). Jour. Anat. 56: 143-155.

THORGAARD, G.H. 1983. Chromosome set manipulation and sex control in fish, p.405-434. In: W.S. Hoar; D.J. Randall & E.M. Donaldson (Eds). Fish physiology. New York, Academic Press, vol. 9B.

UTTER, F.M.; O.W. JOHNSON; G.H. THORGAARD & P.S. RABINOVITCH. 1983. Measurement and potencial aplications of induced triploidy in Pacific salmon. Aquaculture 35: 125-135.

VLRTANEN, E.; L. FORSMAN & A. SUNDBY. 1990. Triploidy decreases the aerobic swimming capacity of rainbow trout (Salmo gairdneri). Comp. Biochem. Physiol. 96A(1): 117-121.

WINTROBE, M.M. 1934. Variations in the size and hemoglobin content of erylhrocytes in the blood of various vertebrates. Folia Hematologica 51: 32-49.

Recebido em 29.VIII.1997; aceito em 01.XII.1996.

  • BECK, M.L. & C.J. BIGGERS. 1983. Erythrocyte measurements of diploid and triploid Ctenophryngodon idella x Hypophthalmichthys nobilis hibrids. Jour. Fish Biol. 22: 497-502.
  • BENFEY, T.J. & A.M. Sutterlin. 1984a. Oxygen utilization by triploidy landlocked Atlantic salmon (Salmo salar L.). Aquaculture 42: 69-73.
  • ______. 1984b. The haematology of triploid landlocked Atlantic salmon, Salmo salar L. Jour. Fish Biol. 24 (3): 333-338.
  • BENFEY, T.J.; A.M. SUTTERLIN; R.J. & THOMPSON. 1984. Use of erythrocyte measurements to identify triploid salmonids. Can. Jour. Fish. Aquat. Sci. 41 (6): 980-984.
  • BYE, V.J. & R.F. LINCOLN. 1986. Comercial methods for the control of sexual maturation in rainbow trout (Salmo gairdneri R.). Aquaculture 57: 299-309.
  • CHERPAS, N.B. 1966. Natural triploiy in females of the unisexual form of goldfish (Carassius auratus gibelio Bloch). Soviet Genet. 2 (5): 9-13.
  • CHOURROUT, D. 1980. Thermal induetion of diploid gynogenesis and triploidy in the eegs of the rainbow trout (Salmo gairdneri Richardson). Reprod. Nutr. Dévelop. 20 (3): 727-733.
  • CIMINO, M.C. 1973. Karyotypes and erythrocyte size of some diploid and triploid fishes of the genus Poeciliopsis. Jour. Fish. Res. Bd. Can. 30: 1736-1737.
  • COLLIER, H.B. 1944. The standardization of blood haemoglobin determinations. Can. Med. Ass. Jour. 50: 550-552.
  • COSTA NETO, P.L. 1977. Estatística. São Paulo, Edgard Blüncher, 246p.
  • Donaldson, E.M. & G.A. Hunter. 1982. Sex control in fish with particular reference to salmonids. Can. Jour. Fish. Aquat. Sci 39: 99-110.
  • GOLDENFARB, P.B.; F.P. BOWYER; E. HALL & E. BROSIUS. 1971. Reproducibility in the hematology laboratory: The micro-hematocrit determinations. Amer. Jour. clin. Pathol. 56 (1): 35-39.
  • GRAHAM, M.S.; G.L. FLETCHER & T.J. BENFEY. 1985. Effect of triploidy on blood oxygen eontent of Atlantic salmon. Aquaculture 50: 133-139.
  • HARDIG, J. & L.B. HOUGLUND. 1984. Seasonal variation in blood components of reared Baltic salmon, Salmo salar L. Jour. Fish Biol. 24 (5): 565-579.
  • HARVEY, W.R. 1987. User's guide for LSMLMW: Mixed model-version. Ohio State University, 59p.
  • IHSSEN, P.E.; L.R. MCKAY; I. MCMILLAN & R.B. PHILLIPS. 1990. Ploidy manipulation and gynogenesis in fishes: citogenetic and fisheries applications. Trans. Amer. Fish. Soc. 119: 698-717.
  • LINCOLN, R.S & A.P. SCOTT. 1984. Sexual maturation in triploid rainbow trout, Salmo gairdneri Richardson. Jour. Fish Biol. 25: 385-392.
  • MCCARTHY, D.H.; J.P. STEVENSON & M.S. ROBERTS. 1973. Some blood parameters of the rainbow trout (Salmo gairdneri Richardson) I. The Kamloops variety. Jour. Fish Biol. 5: 1-8.
  • MEADE, T.L. & S.J. PERRONE. 1980. Selective haematological parameters in steelhead trout, Salmo gairdneri Richardson. Jour. Fish Biol. 17 (1): 9-12.
  • PAIVA, M.J.T.R. 1991. Características sangüíneas da pirapitinga do sul, Brycon sp., sob condições experimentais de criação intensiva. Braz. Jour. Vet. Res. Anim. Scie. 28 (2): 141-153.
  • PAIVA, P.; H.M. GODINHO; C.S.R. MAINARDES-PINTO; Y.A. TABATA & R.G. LEITE. 1985. Comportamento reprodutivo de truta arco-íris, Salmo irideus Gibbons (Osteichithyes, Salmonidae), em cultivo intensivo. Bol. Inst. Pesca 12 (4):61-70.
  • PALÁCIOS, L.; G. VISCOR; J. PALOMEQUE. 1987. Comparative study of two methods to determine erythrocyte dimensions in vertebrates. An. Fac. Vet. León 33: 53-60.
  • PICKERING, A.D. 1986. Changes in blood cell composition of the brown trout, Salmo trutta L., during the spawning season. Jour. Fish Biol. 29: 335-347.
  • PURDOM, C. E. 1983. Genetic engineering by the manipulation of chromosomes. Aquaculture 33: 287-300.
  • RANZANI-PAIVA, M.J.T. 1995. Características hematológicas da tainha, Mugil platamis Günther, 1880 (Osteichthyes, Mugilidae) da região estuarino-lagunar de Cananéia, SP (Lat. 25º00"S - Long. 47º55'W). Bol. Inst. Pesca 2 (1): 1-22.
  • RANZANI-PAIVA, M.J.T. & H.M. GODINHO. 1985. Estudos hematológicos em curimbatá, Prochilodas scrofa Steindachner, 1881 (Osteichthyes, Cyprinifor-mes, Prochilodontidae). Série vermelha. Bol. Inst. Pesca 12 (2): 25-35.
  • ROSEFIELD, G. 1947. Corante pancrômico para hematologia e citologia clínica. Nova combinação dos componentes do May-Grünwald e do Giemsa num só corante de emprego rápido. Mem. Inst. Butantan 20: 329-334
  • SEZAKI, K.; S. WATANABE & K. HASHIMOTO. 1988. Haematological parameters and erythocyte enzyme activities assoeiatcd with increase in ploidy status of the spinous loach, Cobitis biwae Jordan and Snyder. Jour. Fish Biol. 32 (1): 149-150.
  • SMALL, S.A & T.J. BENFEY. 1987. Cell size in triploid salmon. Jour. Exp. Zool. 241: 339-342.
  • SWARUP, H. 1959. Effect of triploidy on the blood size, general organization and cellular strueture in Gasterosteus aculeatus (L). Jour. Anat. 56: 143-155.
  • THORGAARD, G.H. 1983. Chromosome set manipulation and sex control in fish, p.405-434. In: W.S. Hoar; D.J. Randall & E.M. Donaldson (Eds). Fish physiology. New York, Academic Press, vol. 9B.
  • UTTER, F.M.; O.W. JOHNSON; G.H. THORGAARD & P.S. RABINOVITCH. 1983. Measurement and potencial aplications of induced triploidy in Pacific salmon. Aquaculture 35: 125-135.
  • VLRTANEN, E.; L. FORSMAN & A. SUNDBY. 1990. Triploidy decreases the aerobic swimming capacity of rainbow trout (Salmo gairdneri). Comp. Biochem. Physiol. 96A(1): 117-121.
  • WINTROBE, M.M. 1934. Variations in the size and hemoglobin content of erylhrocytes in the blood of various vertebrates. Folia Hematologica 51: 32-49.

Datas de Publicação

  • Publicação nesta coleção
    06 Jul 2009
  • Data do Fascículo
    Dez 1998

Histórico

  • Aceito
    01 Dez 1996
  • Recebido
    29 Ago 1997
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