The objective of this work was to develop a method for the micro-propagation of the China Rose. Two experiments were conducted using explants from plants grown in vitro. In the first experiment, to test multiplication, the explants were separately seeded onto an MS medium with BAP, CIN and TCT, at concentrations of 0.00, 0.01, 0.10, 1.00 and 10.00 mg L-1 with 15 treatments and 20 explants per treatment. After 80 days it was found that BAP at a concentration of 1.0 mg L-1 favoured the emission of buds and the greatest fresh weight of the explants. For rooting, different formulation concentrations of the macronutrient salts in the MS medium (25, 50, 75 and 100%) were used, combined with different concentrations of AIB (0.0, 0.1 and 1.0 mg L-1), forming a 4 x 3 factorial in a completely randomised design for both experiments. After 60 days, the best development of those plants with normal roots was observed when using the MS medium in its normal concentration (100%) without AIB. For multiplication and rooting in vitro of the China Rose therefore, MS medium with 1.0 mg L-1 BAP, and MS medium with 100% of hormone free salts should be used, respectively.
Micropropagation; Roots; Rosa chinensis
