Genetic diversity of recombinant inbred lines of Phaseolus vulgaris L.¹

Diversidade genética de linhagens endogâmicas recombinantes de Phaseolus vulgaris L.

ABSTRACT

Considred a more accessible protein source for the population, common beans are consumed daily, mainly in developing countries, in the tropical and subtropical regions of the planet. Studies that use both morphological descriptors and molecular markers have been used in plant breeding programs for common beans. This article aims to report the study of the diversity of recombinant inbred lines and their parents using morphologial descriptors and SSR markers. 22 recombinant inbreeding lines were evaluated derived from the BRS Agreste parents and the black-01 line.For the characterization, 18 microsatellite markers and the following morphological descriptors were used: presence of anthocyanin, number of days after germination, days for flowering, wing color, standard color, seed color, tegument color, seed brightness, commercial group. For molecular characterization, seven markers were used that presented polymorphism The dissimilarity values, using morphological data, ranged from 0 to 8.5; organizing the lineages into three distinct groups. In the molecular analyzes, of the 18 primers tested, 11 were monomorphic and 7 presented polymorphism. The dissimilarity values for molecular analysis ranged from 0 to 0.8; with an average of 0.57. In addition to being informative, these markers are associated with important characteristics for common beans. Joint analysis of the data presented dissimilarity values ranging from 0.0 to 12.5, with an average of 6.89, forming three groups. The joint analysis of morphological and molecular data was efficient to characterize and distinguish inbred lines. The molecular markers used were suitable to the present study. The lines 18 and 19 can be considered duplicate because in all analyzes they remained together.

Key words:
Dissimilarity; Common Bean; SSR Marker; Genetic improvement; SSD