Exchange of germoplasm and genetic diversity to enrichment the <i>Desmanthus</i> genebank

Silva, Ana V. C. da; Nascimento, Isa M. R. do; Rangel, José H. A.; Pereira, Camila S. A.; Muniz, Evandro N.

doi:10.1590/1983-21252023v36n104rc

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Sumário

Agronomy • Rev. Caatinga 36 (1) • Jan-Mar 2023 • https://doi.org/10.1590/1983-21252023v36n104rc copy

Exchange of germoplasm and genetic diversity to enrichment the Desmanthus genebank

Intercâmbio de germoplasma e diversidade genética para enriquecimento do bag Desmanthus

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The genus Desmanthus presents wide ecogeographical distribution and complex taxonomy. Desmanthus pernambucanus (L.) Thellung, popularly known in Brazil as Jureminha, is a leguminous species native to the Northeast region of Brazil, which stands out by its high protein content, resistance to droughts, and by presenting no toxicity to animals. The objective of this work was to evaluate the genetic diversity of 15 progenies from the Commonwealth Scientific and Industrial Research Organization (CSIRO; Australia), through germplasm exchange for enrichment of the Desmanthus genebank of Embrapa Tabuleiros Costeiros, in Nossa Senhora das Dores, SE, Brazil. Fifteen ISSR markers were used, from which 8 were selected. The progenies identified as 242, 245, 246, 255, and 268 were the most genetically distant, and the most recommended to be introduced to the genebank.

Keywords:
Jureminha; Ex situ conservation; Forage; Native legume; Genetic diversity

Primers	Sequence	AT (°C)
ISSR 1	CAC ACA CAC ACA GG	51
ISSR 2	CTC TCT CTC TCT CTC TAC	51
ISSR 3	CTC TCT CTC TCT CTC TTG	51
ISSR 4	CAC ACA CAC ACA AC	51
ISSR 5	CTC TCT CTC TCT CTC TGC	44
ISSR 6	CAC ACA CAC ACA AG	44
ISSR 7	CAC ACA CAC ACA GT	44
ISSR 8	GAG AGA GAG AGA GG	39
ISSR 9	GTG TGT GTG TGT CC	39
ISSR 10	GAG AGA GAG AGA CC	39
ISSR 11	GTG TGT GTG TGT CC	39
ISSR 12	CAC CAC CAC GC	39
ISSR 13	GAG GAG GAG GC	39
ISSR 15	CTC CTC CTC GC	52

ISSR primers	Total number of fragments	Number of polymorphic fragments	Polimorphism percentage (%)
ISSR 2	7	5	71
ISSR 3	3	2	66
ISSR 4	4	2	50
ISSR 5	6	5	83
ISSR 7	7	6	85
ISSR 12	2	0	0
ISSR 13	6	6	100
ISSR 15	3	1	33
Total	38	27

Number of individuals	N_A	N_E	I	H_E	H_O
15	1.76	1.49	0.42	0.28	0.29

Progenies
Progenies	243	269	249	270	242	255	246	239	245	268	261	263	251	257	247
243	1.00
269	0.97	1.00
249	0.80	0.82	1.00
270	0.87	0.90	0.79	1.00
242	0.65	0.67	0.58	0.74	1.00
255	0.55	0.57	0.48	0.63	0.85	1.00
246	0.50	0.52	0.53	0.57	0.61	0.48	1.00
239	0.76	0.78	0.74	0.80	0.68	0.63	0.57	1.00
245	0.61	0.63	0.55	0.64	0.70	0.80	0.57	0.64	1.00
268	0.61	0.63	0.55	0.64	0.70	0.64	0.57	0.59	0.81	1.00
261	0.88	0.90	0.79	0.87	0.69	0.59	0.53	0.87	0.66	0.66	1.00
263	0.83	0.80	0.76	0.76	0.61	0.52	0.43	0.71	0.53	0.53	0.82	1.00
251	0.77	0.74	0.70	0.71	0.65	0.55	0.50	0.66	0.61	0.61	0.76	0.88	1.00
257	0.91	0.88	0.73	0.84	0.63	0.53	0.48	0.74	0.59	0.59	0.85	0.86	0.80	1.00
247	0.88	0.85	0.81	0.82	0.61	0.52	0.52	0.82	0.58	0.58	0.88	0.89	0.83	0.91	1.00

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E-mail: caatinga@ufersa.edu.br

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[1] *Corresponding author: <ana.veruska@embrapa.br>