This study aimed to assess patterns of isozyme Esterase (EST - EC 3.1.1.1), Acid Phosphatase (ACP - EC 3.1.3.2), Malate Dehydrogenase (MDH - EC 1.1.1.37), Alcohol Dehydrogenase (ADH - EC 1.1 .1.1) and Glutamate Oxalacetate Transaminase (GOT - EC 2.6.1.1) of two barley cultivars (MN 721 and Scarlett). The seeds were supplied by the company Westermann, located in the Piratini, RS, which had undertaken the cultivation of barley in 2007. Were collected in three seasons with different percentages of moisture and dried at 13% and stored in cold. The five isozyme systems analyzed showed variations in expression, especially when compared to seeds and seedlings. It was concluded that there is variation in the pattern of expression of the enzymes EST, ACP, MDH, ADH and GOT between seeds and seedlings. The expression of EST and GOT enzymes were little and very influenced by cropping season, respectively.
Hordeum vulgare L.; electrophoresis; protein extraction
