The in vitro culture of zygotic embryos represents a promising technique to study the embryonic development and dormancy in seeds. The objective of this study was to determine the effect of plant growth regulators, 6-benzylaminopurine (BAP) and naphthalene acetic (NAA), in the morphogenetic potential of in vitro zygotic embryos of mulungu. Mature zygotic embryos from seeds were either used as a whole or sectioned in plumule, radicle and intermediate region, and subsequently inoculated in WPM medium supplemented with combinations of BAP (0.0, 2.0, 4, 0, 8.0, 12.0 and 16.0 µM) and NAA (0.0, 1.0 and 2.0 µM), added with 87.65 mM sucrose and 0.7% agar. After 30 days, the following parameters were evaluated: regeneration percentage of embryos and plumularapice, number of buds, number of leaves, aerial part length of buds, number of roots and percentage of callifrom the intermediate region and radicle. It was feasible the in vitro regeneration of mulungu from plumule explant and whole zygotic embryos in WPM medium supplemented with 4.0 µM BAP. Moreover, intermediate and radicle regions promoted the formation of compact calli (96.06%)by combining10.63 µM BAP and 2.0 µM NAA.
6-benzylaminopurine; morphogenesis; mulungu; naphthalene acetic
