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Collection of tracheal aspirate: safety and microbiological concordance between two techniques* * Extracted from part of the dissertation "Colheita de aspirado traqueal para o diagnóstico microbiológico de pneumonia associada à ventilação mecânica: comparação de duas técnicas" , Program of Health and Development in the Midwest Region, Federal University of Mato Grosso do Sul, 2013. 1 Master’s from the Program of Health and Development in the Midwest Region, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil 2 Master’s and Doctoral Advisor, Program of Health and Development in the Midwest Region and Master’s in Nursing, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil 3 Assistant professor, Nursing Course, Federal University of Mato Grosso do Sul, Coxim, MS, Brazil 4 Professor, Doctor, College of Odontology of Ribeirão Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil 5 Pharmaceutical Biochemistry, Laboratory of Microbiology, University Hospital Center, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil 6 Assistant professor, Nursing Course, Federal University of Mato Grosso do Sul, Três Lagoas, MS, Brazil

Recolección de aspirado traqueal: seguridad y concordancia microbiológica entre dos técnicas

Objective: To evaluate the safety of the performance of the traditional and protected collection techniques of tracheal aspirate and to identify qualitative and quantitative agreement of the results of microbiological cultures between the techniques. Method: Clinical, prospective, comparative, single-blind research. The sample was composed of 54 patients of >18 years of age, undergoing invasive mechanical ventilation for a period of ≥48 hours and with suspected Ventilator Associated Pneumonia. The two techniques were implemented in the same patient, one immediately after the other, with an order of random execution, according to randomization by specialized software. Results: No significant events occurred oxygen desaturation, hemodynamic instability or tracheobronchial hemorrhage (p<0.05) and, although there were differences in some strains, there was qualitative and quantitative agreement between the techniques (p<0.001). Conclusion: Utilization of the protected technique provided no advantage over the traditional and execution of both techniques was safe for the patient.


Pneumonia, ventilator-associated; Culture techniques; Infection; Intensive care; Nursing care; Patient´s safety


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