A sample of 125 sera from children aged 0-10 years old from Goiânia, Goiás, Brazil, yielded an 82.4% prevalence rate of antibodies to rotavirus (through an enzyme immunoassay). In this study, the 1-3 year old group shows the highest risk of infection with rotavirus. No sex difference was evident. Regional seroepidemiological data is of the utmost importance in order to identify the behaviour of the virus in the population at risk, specially with the advent of a vaccine for the agent. The enzyme imu- noassay test was compared to counterimmuno-electro-osmophoresis and was shown to be more sensitive for the detection of antibodies.