Sensibilidade de leveduras do gênero Candida, isoladas de pacientes com câncer, a antifúngicos poliênicos

Alves, Sydney Hartz; Cury, Ariete Emily

doi:10.1590/S0036-46651992000300012

Resumos

Por meio da determinação da CIM (Concentração Inibitória Mínima) e da CFM (Concentração Fungicida Mínima), os autores compararam a sensibilidade de amostras de Candida isoladas de pacientes com câncer, com as de cepas isoladas de pacientes sem câncer, frente à anfotericina B e a nistatina. Os autores não verificaram diferenças significativas entre os dois grupos estudados e discutem o fenômeno da resistência de Candida a antifungicos poliênicos

Candida; Sensibilidade; Antifungicos

Candida strains susceptibility from cancer patients were compared with Candida strains susceptibility from patients, without cancer by MIC (Minimal Inhibitory Concentration) and MFC (Minimal Fungicidal Concentration) to Amphotericin B and Nystatin. Broth dilution method and Agar dilution method were the procedure employed. The authors find no significant differences between the studied groups. The problem of Candida resistance to polyene antifungals is discussed

ENSAIOS TERAPÊUTICOS

Sensibilidade de leveduras do gênero Candida, isoladas de pacientes com câncer, a antifúngicos poliênicos

Candida from cancer patients: susceptibility "in vitro" to polyene antifungal agents

Sydney Hartz Alves^I; Ariete Emily Cury^II

^ILaboratório de Análises Clínicas-Hospital Universitário; Universidade Federal de Santa Maria; Campus da UFSM. 97100, Santa Maria, RS, Brasil

^IIDepartamento de Análises Clínicas; Faculdade de Ciências Farmacêuticas; Universidade de São Paulo. Cx. Postal 30786, São Paulo, SP, Brasil

RESUMO

Por meio da determinação da CIM (Concentração Inibitória Mínima) e da CFM (Concentração Fungicida Mínima), os autores compararam a sensibilidade de amostras de Candida isoladas de pacientes com câncer, com as de cepas isoladas de pacientes sem câncer, frente à anfotericina B e a nistatina. Os autores não verificaram diferenças significativas entre os dois grupos estudados e discutem o fenômeno da resistência de Candida a antifungicos poliênicos.

Unitermos: Candida, Sensibilidade, Antifungicos.

SUMMARY

Candida strains susceptibility from cancer patients were compared with Candida strains susceptibility from patients, without cancer by MIC (Minimal Inhibitory Concentration) and MFC (Minimal Fungicidal Concentration) to Amphotericin B and Nystatin. Broth dilution method and Agar dilution method were the procedure employed.

The authors find no significant differences between the studied groups. The problem of Candida resistance to polyene antifungals is discussed.

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Recebido para publicação em 20/09/1991.

Aceito para publicação em 17/03/1992.

1. AHEARN, D.G. & McGLOHN, M.S. - In vitro susceptibilities of sucrose negative Candida tropicalis, Candida lusitaniae, and Candida norvegensis to amphotericin B, 5-fluorocitosine, miconazole, and ketoconazole. J. clin. Microbiol., 19: 412-416, 1984.
2. BODENHOFF, J. - Development of strains of genus Candida and genus Torulopsis resistant to antimycotics. Acta path. microbiol. scand., 75: 622-630, 1969.
3. BRAJTBURG, J.; ELBERG, S.; KOBAYASHI, G.S. & MEDOFF, G. - Interference with effects of amphotericin on C. albicans cells by 2-chloroesthyl-1-nitrosoureas. Antimicrob. Agents Chemother., 15: 763-768, 1988.
4. BRINCKER, H. - Prevention of mycosis in granulocytopenic patients with prophylatic ketoconazole treatment. Mykosen, 26: 242-247, 1983.
5. CURY, A.E.; MICHE, M.P. & MINAMI, P.S. - Leveduras isoladas de pacientes com câncer: incidęncia e sensibilidade a antibióticos polięnicos. Rev. Microbiol. (S. Paulo), 20: 102-107, 1989.
6. DICK, J.D.; MERZ, W.G. & SARAL, R. - Incidence of polyene resistant yeasts recovered from clinical specimens. Antimicrob. Agents Chemother., 18: 158-163 1980.
7. DICK, J.D.; ROSENGARD, B.R.; MERZ, W.G.; STUART, R.K.; HUTCHINS, G.M. & SARAL, R. - Fatal disseminated candidiasis due to amphotericin B resistant C. guilliermondii. Ann. intern. Med., 102: 67-68, 1985.
8. DRUTZ, D.J. & LEHRER, R.I. - Development of amphotericin B - resistant Candida tropicalis in a patient with defective leukocyte function. Amer. J. med. Sci., 276: 77-92, 1978.
9. GHANNOUM, M.A. & Al-KHARS, A. - Effect of antineoplastic agents on the growth, and ultrastructure of Candida albicans. Mykosen, 27: 452-464, 1984.
10. GHANNOUM, M.A.; SHARIF, H.F. & Al-GHARREER, H. - Sensitivity of clinical yeast isolates in Kwait against a number of antifungal agents. Mykosen, 27: 402-410, 1984.
11. GHANNOUM, M.A. - Effects of antineoplastic agents on growth, morphology and metabolism of Torulopsis glabrata. Mycopathologia (Den Haag), 95: 175-181, 1986.
12. GUINET, R.; CHASNAS, J.; GOULLIER, A.; BONNEFOY, G. & AMBROISE-THOMAS, P. - A fatal septicemia due to amphotericin B resistant Candida lusitaniae. J. clin. Microbiol., 18: 443-444, 1983.
13. HOLT, R.J. - Laboratory tests of antifungal drugs. J. clin. Path., 28: 767-774, 1985.
14. HOWARTH, W.R.; TEWARI, R.P. & SOLOTOROVSKI, M. - Comparative in vitro antifungal activity of amphotericin B and amphotericin B methyl ester. Antimicrob. Agents Chemother., 7: 58-63, 1975.
15. HUSSAIN QUADRI, S.M.H.; FLOURNOY, D.J.; QUADRI, S.G.M. & RAMIREZ, E.G. - Susceptibility of clinical isolates of yeasts to antifungal agents. Mycopathologia (Den Haag), 95: 183-187, 1986.
16. KOSTIALA, I.A.A. & KOSTIALA, I. - Broth dilution and disc diffusion methods in the susceptibility testing of pathogenic Candida albicans against four antimicotics. Mycopathologia (Den Haag), 87: 121-127, 1984.
17. LAND, G.A.; HILME, K.L. & CHAFFIN, W.L. - The effects of selected antineoplastic agents on the morphology of Candida albicans 5865. Canad. J. Microbiol., 28: 812-818, 1980.
18. MARTIN, M.V.; Al-TIKRITI, U. & BRAMLEY, P.A. - Yeast flora of mouth and skin during and after irradiation for oral and laryngeal cancer. Amer. J. Med., 14: 457-467, 1981.
19. MERZ, W.G. & SANFORD, G.R. - Isolation and characterization of a polyene-resistant variant of Candida tropicalis. J. clin. Microbiol., 9: 677-680, 1979.
20. PAPPAGIANIS, D.; COLLINS, M.S.; HECTOR, R. & REMINGTON, J. - Development of resistance to amphotericin B in Candida lusitaniae infecting a human. Antimicrob. Agents Chemother., 16: 123-126, 1979.
21. SAFE, L.M.; SAFE, S.H.; SUBDEN, R.E. & MORRIS, D.C. - Sterols content and polyene antibiotic resistance in isolates of Candida krusei, Candida parakrusei and Candida tropicalis. Canad. J. Microbiol., 23: 398-401, 1977.
22. SCHOLER, H.J. & POLAK, A. - Resistance to systemic antifungal agents. In: BRYAN, L.E., ed. Antimicrobial drug resistance. Orlando, Academic Press, 1984. p. 393-460.
23. SHADOMY, S.; ESPINEL-INGROFF, A. & CARTWRIGHT, R. - Laboratory studies with antifungal agents: susceptibility tests and bioassays. In: LENNETE, E.H.; BALLOWS, A.; HAUSLER Jr., W.J. & SHADOMY, H.J., ed. Manual of Clinical Microbiology. 4 ed. Washington, American Society of Microbiology, 1985. p. 991-999.
24. SOKOL-ANDERSON, M.L.; SLIGH, J.E.; ELBERG, S.; BRAJBURG, J.; KOBAYASHI, G. & MEDOFF, G. - Role of cell defense against oxidative damage in the resistance of Candida albicans to the killing effects of amphotericin B. Antimicrob. Agents Chemother, 32: 702-705, 1988.
25. VAN DER WALT, J.P. & YARROW, D. - Methods for the isolation, maintenance, classification and identification of yeasts. In: KREGERVAN RIJ, N.J.W., ed., The Yeasts. Amsterdan, Elsevier, 1984. p. 45-104.
26. WOODS, R.A.; BARD, M.; JACKSON, I.E. & DRUTZ, D.J. - Resistance of polyene antibiotics and correlated sterol changes in two isolates of Candida tropicalis from patient with an amphotericin B-resistant funguria. J. infect. Dis., 129: 53-58, 1974.

Datas de Publicação

Publicação nesta coleção
11 Set 2006
Data do Fascículo
Jun 1992

Histórico

Aceito
17 Mar 1992
Recebido
20 Set 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. AHEARN, D.G. & McGLOHN, M.S. - In vitro susceptibilities of sucrose negative Candida tropicalis, Candida lusitaniae, and Candida norvegensis to amphotericin B, 5-fluorocitosine, miconazole, and ketoconazole. J. clin. Microbiol., 19: 412-416, 1984.

[2] 2. BODENHOFF, J. - Development of strains of genus Candida and genus Torulopsis resistant to antimycotics. Acta path. microbiol. scand., 75: 622-630, 1969.

[3] 3. BRAJTBURG, J.; ELBERG, S.; KOBAYASHI, G.S. & MEDOFF, G. - Interference with effects of amphotericin on C. albicans cells by 2-chloroesthyl-1-nitrosoureas. Antimicrob. Agents Chemother., 15: 763-768, 1988.

[4] 4. BRINCKER, H. - Prevention of mycosis in granulocytopenic patients with prophylatic ketoconazole treatment. Mykosen, 26: 242-247, 1983.

[5] 5. CURY, A.E.; MICHE, M.P. & MINAMI, P.S. - Leveduras isoladas de pacientes com câncer: incidęncia e sensibilidade a antibióticos polięnicos. Rev. Microbiol. (S. Paulo), 20: 102-107, 1989.

[6] 6. DICK, J.D.; MERZ, W.G. & SARAL, R. - Incidence of polyene resistant yeasts recovered from clinical specimens. Antimicrob. Agents Chemother., 18: 158-163 1980.

[7] 7. DICK, J.D.; ROSENGARD, B.R.; MERZ, W.G.; STUART, R.K.; HUTCHINS, G.M. & SARAL, R. - Fatal disseminated candidiasis due to amphotericin B resistant C. guilliermondii. Ann. intern. Med., 102: 67-68, 1985.

[8] 8. DRUTZ, D.J. & LEHRER, R.I. - Development of amphotericin B - resistant Candida tropicalis in a patient with defective leukocyte function. Amer. J. med. Sci., 276: 77-92, 1978.

[9] 9. GHANNOUM, M.A. & Al-KHARS, A. - Effect of antineoplastic agents on the growth, and ultrastructure of Candida albicans. Mykosen, 27: 452-464, 1984.

[10] 10. GHANNOUM, M.A.; SHARIF, H.F. & Al-GHARREER, H. - Sensitivity of clinical yeast isolates in Kwait against a number of antifungal agents. Mykosen, 27: 402-410, 1984.

[11] 11. GHANNOUM, M.A. - Effects of antineoplastic agents on growth, morphology and metabolism of Torulopsis glabrata. Mycopathologia (Den Haag), 95: 175-181, 1986.

[12] 12. GUINET, R.; CHASNAS, J.; GOULLIER, A.; BONNEFOY, G. & AMBROISE-THOMAS, P. - A fatal septicemia due to amphotericin B resistant Candida lusitaniae. J. clin. Microbiol., 18: 443-444, 1983.

[13] 13. HOLT, R.J. - Laboratory tests of antifungal drugs. J. clin. Path., 28: 767-774, 1985.

[14] 14. HOWARTH, W.R.; TEWARI, R.P. & SOLOTOROVSKI, M. - Comparative in vitro antifungal activity of amphotericin B and amphotericin B methyl ester. Antimicrob. Agents Chemother., 7: 58-63, 1975.

[15] 15. HUSSAIN QUADRI, S.M.H.; FLOURNOY, D.J.; QUADRI, S.G.M. & RAMIREZ, E.G. - Susceptibility of clinical isolates of yeasts to antifungal agents. Mycopathologia (Den Haag), 95: 183-187, 1986.

[16] 16. KOSTIALA, I.A.A. & KOSTIALA, I. - Broth dilution and disc diffusion methods in the susceptibility testing of pathogenic Candida albicans against four antimicotics. Mycopathologia (Den Haag), 87: 121-127, 1984.

[17] 17. LAND, G.A.; HILME, K.L. & CHAFFIN, W.L. - The effects of selected antineoplastic agents on the morphology of Candida albicans 5865. Canad. J. Microbiol., 28: 812-818, 1980.

[18] 18. MARTIN, M.V.; Al-TIKRITI, U. & BRAMLEY, P.A. - Yeast flora of mouth and skin during and after irradiation for oral and laryngeal cancer. Amer. J. Med., 14: 457-467, 1981.

[19] 19. MERZ, W.G. & SANFORD, G.R. - Isolation and characterization of a polyene-resistant variant of Candida tropicalis. J. clin. Microbiol., 9: 677-680, 1979.

[20] 20. PAPPAGIANIS, D.; COLLINS, M.S.; HECTOR, R. & REMINGTON, J. - Development of resistance to amphotericin B in Candida lusitaniae infecting a human. Antimicrob. Agents Chemother., 16: 123-126, 1979.

[21] 21. SAFE, L.M.; SAFE, S.H.; SUBDEN, R.E. & MORRIS, D.C. - Sterols content and polyene antibiotic resistance in isolates of Candida krusei, Candida parakrusei and Candida tropicalis. Canad. J. Microbiol., 23: 398-401, 1977.

[22] 22. SCHOLER, H.J. & POLAK, A. - Resistance to systemic antifungal agents. In: BRYAN, L.E., ed. Antimicrobial drug resistance. Orlando, Academic Press, 1984. p. 393-460.

[23] 23. SHADOMY, S.; ESPINEL-INGROFF, A. & CARTWRIGHT, R. - Laboratory studies with antifungal agents: susceptibility tests and bioassays. In: LENNETE, E.H.; BALLOWS, A.; HAUSLER Jr., W.J. & SHADOMY, H.J., ed. Manual of Clinical Microbiology. 4 ed. Washington, American Society of Microbiology, 1985. p. 991-999.

[24] 24. SOKOL-ANDERSON, M.L.; SLIGH, J.E.; ELBERG, S.; BRAJBURG, J.; KOBAYASHI, G. & MEDOFF, G. - Role of cell defense against oxidative damage in the resistance of Candida albicans to the killing effects of amphotericin B. Antimicrob. Agents Chemother, 32: 702-705, 1988.

[25] 25. VAN DER WALT, J.P. & YARROW, D. - Methods for the isolation, maintenance, classification and identification of yeasts. In: KREGERVAN RIJ, N.J.W., ed., The Yeasts. Amsterdan, Elsevier, 1984. p. 45-104.

[26] 26. WOODS, R.A.; BARD, M.; JACKSON, I.E. & DRUTZ, D.J. - Resistance of polyene antibiotics and correlated sterol changes in two isolates of Candida tropicalis from patient with an amphotericin B-resistant funguria. J. infect. Dis., 129: 53-58, 1974.

Brasil

Brasil

Sensibilidade de leveduras do gênero Candida, isoladas de pacientes com câncer, a antifúngicos poliênicos

Candida from cancer patients: susceptibility "in vitro" to polyene antifungal agents

Resumos

Datas de Publicação

Histórico