Sera from 47 Cannis familiaris, 9 Felis cattus, 64 Didelphis marsupialis, 9 Dasypus novemcinctus, 4 Cabassous tatouay and 29 Rattus rat- tus were examined for anti-Toxoplasma gondii antibodies by the indirect immunofluorescence reaction. Only two species were positives: C. familiaris (63.8%) and D.m. aurita (4.7%). For a better knowledge of the importance of the various animals species in toxoplasmosis dissemination the Authors suggest further researchs on this subject.