Efeito de concentrações subinibitórias de penicilina sobre antígenos de estreptococos do grupo G

Castro, Angela C. D. de; Benchetrit, Leslie C.; Oliveira, César M. de

doi:10.1590/S0036-46651986000400005

Resumos

O efeito de concentrações subinibitórias de penicilina sobre a produção do antígeno grupo-específico e da hialuronidase extracelular foi avaliado em uma amostra de estreptococo pertencente ao grupo G de Lancefield. Em todas as concentrações uma maior quantidade de antígeno grupo-específico foi extraída das células e a atividade específica de hialuronidase se mostrou aumentada em até 1400% nos sobrenadantes das culturas. O maior aumento na expressão de ambos os antígenos foi observado em 1/2 da CMI.

Estreptococos grupo G; Penicilina; Antígenos estreptocócicos

The effect of subinhibitory concetrations of penicillin on a strain of streptococcus belonging to Lancefield group G was studied. Production of the group-specific antigen and extra- cellular hyaluronidase by drug-exposed cells were examined. At all concentrations levels a higher amount of group-specific antigen was extracted from cells and increases of up to 1400% in the specific activity of hyaluronidase were determined in culture supernates. The higher increase in the expression of both antigens was observed at 1/2 MIC.

Efeito de concentrações subinibitórias de penicilina sobre antígenos de estreptococos do grupo G

Effect of subinhibitory concentrations of penicillin on antigens of group G streptococci

Angela C. D. de Castro; Leslie C. Benchetrit; César M. de Oliveira

Centro de Referência para Estreptococos, Instituto de Microbiologia da UFRJ. Caixa Postal 68040. CEP 21.944, Rio de Janeiro, RJ., Brasil

RESUMO

O efeito de concentrações subinibitórias de penicilina sobre a produção do antígeno grupo-específico e da hialuronidase extracelular foi avaliado em uma amostra de estreptococo pertencente ao grupo G de Lancefield.

Em todas as concentrações uma maior quantidade de antígeno grupo-específico foi extraída das células e a atividade específica de hialuronidase se mostrou aumentada em até 1400% nos sobrenadantes das culturas. O maior aumento na expressão de ambos os antígenos foi observado em 1/2 da CMI.

Unitermos: Estreptococos grupo G Penicilina Antígenos estreptocócicos.

SUMMARY

The effect of subinhibitory concetrations of penicillin on a strain of streptococcus belonging to Lancefield group G was studied. Production of the group-specific antigen and extra- cellular hyaluronidase by drug-exposed cells were examined. At all concentrations levels a higher amount of group-specific antigen was extracted from cells and increases of up to 1400% in the specific activity of hyaluronidase were determined in culture supernates. The higher increase in the expression of both antigens was observed at 1/2 MIC.

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AGRADECIMENTOS

Este estudo recebeu o auxílio da CAPES, CNPq, PINEP, CEPG da UFRJ e Fundação Universitária José Bonifácio.

Recebido para publicação em 20/8/1985.

1. BENCHETRIT, L. C; AVELINO, C. C. & OLIVEIRA, C. M. Effect of subminimal inhibitory concentrations of penicillin on hyaluronidase production by group A streptococci. Zbl. Bakt. Hyg., I. Abt. Orig. A., 251: 152-156, 1981,
2. BENCHETRIT, L. C; PAHUJA, S. L.; GRAY, E. D. & EDSTROM, R. D. A sensitive method for the assay of hyaluronidase activity. Analyt. Biochem., 79: 431-437, 1977.
3. EDSTROM, R. D. A colorimetric method for the determination of mucopolysaccharides and other acidic polymers. Analyt. Biocbem., 29: 421-432, 1969.
4. EL KHOLY, A.; WANNAMAKER, L. W. & KRAUSE, R. M. Simplified extraction procedure for serological grouping of betahemolytic streptococci. Appl. Microbiol., 28: 836-839, 1974.
5. FABER, V. Streptococcal hyaluronidase. Review and the turbidimetric method for its determination. Acta path, microbiol. scand., 31: 345-354, 1952.
6. FABER, V. & ROSENDAL, K. Streptococcal hyaluronidase IV. The effect of penicillin on the production of hyaluronic acid and hyaluronidase by hemolytic streptococci (type 4, group A). Acta. path. microbiol. scand., 37: 286-292, 1955.
7. FERNE, M.; RABINOWITZ, S. B.; DUCHAN, Z. & MICHEL, J. Morphologic and enaymatic changes of beta-hemolytic group A streptococci due to submininal inhibitory concentrations of chloramphenicol, erythromycin and penicillin. In: HOLM, S. E. & CHRISTENSEN, P., eds. Basic concepts of streptococci and streptococcal diseases Chertsey, England, Reedbooks, 1982. p. 148-150.
8. LANCEFIELD, R. C. A serological differentiation of human and other groups of hemolytic streptococci. J. exp. Med., 57: 571-595, 1933.
9. LORIAN, V. Some effects of subinhibitory concentrations of penicillin on the structure and division of staphylococci. Antimicrob. Agents Chemother., 7: 864-870, 1975.
10. LORIAN, V. Effects of subminimum inhibitory concentrations of antibiotics on bacteria. In: LORIAN, V., ed. Antibiotics in laboratory medicine Baltimore, Williams & Wilkins, 1980. p. 342-408.
11. LORIAN, V. & ATKINSON, B. Effects of subinhibitory concentrations of antibiotics on cross walls of cocci. Antimicrob. Agents Chemother., 6: 1043-1055, 1976.
12. MILATOVIC, D. Effect of subinhibitory antibiotic concentrations on the phagocytosis of Staphylococcus aureus. Europ. J. clin. Microbiol., 1: 97-101, 1982.
13. ROSENDAL, K. & FABER, V. Streptococcal hyaluronidase. III. The effect of penicillin on the production of hyluronic acid and hyaluronidase by hemolytic streptococci (type 24, group A). Acta pathol. microbiol. scand., 36: 263-273, 1955a.
14. ROSENDAL, K. & FABER, V. Streptococcal hyaluronidase. V. The effect of penicillin on the production of hyaluronic acid and hyaluronidase by hemolytic streptococci (type 28, group A). Acta patbol. microbiol. scand., 37: 293-299, 1955b.
15. VOSBECK, K.; METT, H.; HUBER, U.; BOHN, J. & PETIGNAT, M. Effects of low concentrations of antibiotics on Escherichia coll adhesion. Antimicrob. Agents Chemother., 21: 864-869, 1982.
16. WASHINGTON II, J. A. & BARRY, A. L. Dilution test procedures. In: LENNETTE, E. H.; SPAULDING, E. H. & TRUANT, J. P., eds. Manual of clinical microbiology Washington, D.C., American Society for Microbiology, 1974. p. 410-417.

Datas de Publicação

Publicação nesta coleção
16 Out 2012
Data do Fascículo
Ago 1986

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BENCHETRIT, L. C; AVELINO, C. C. & OLIVEIRA, C. M. Effect of subminimal inhibitory concentrations of penicillin on hyaluronidase production by group A streptococci. Zbl. Bakt. Hyg., I. Abt. Orig. A., 251: 152-156, 1981,

[2] 2. BENCHETRIT, L. C; PAHUJA, S. L.; GRAY, E. D. & EDSTROM, R. D. A sensitive method for the assay of hyaluronidase activity. Analyt. Biochem., 79: 431-437, 1977.

[3] 3. EDSTROM, R. D. A colorimetric method for the determination of mucopolysaccharides and other acidic polymers. Analyt. Biocbem., 29: 421-432, 1969.

[4] 4. EL KHOLY, A.; WANNAMAKER, L. W. & KRAUSE, R. M. Simplified extraction procedure for serological grouping of betahemolytic streptococci. Appl. Microbiol., 28: 836-839, 1974.

[5] 5. FABER, V. Streptococcal hyaluronidase. Review and the turbidimetric method for its determination. Acta path, microbiol. scand., 31: 345-354, 1952.

[6] 6. FABER, V. & ROSENDAL, K. Streptococcal hyaluronidase IV. The effect of penicillin on the production of hyaluronic acid and hyaluronidase by hemolytic streptococci (type 4, group A). Acta. path. microbiol. scand., 37: 286-292, 1955.

[7] 7. FERNE, M.; RABINOWITZ, S. B.; DUCHAN, Z. & MICHEL, J. Morphologic and enaymatic changes of beta-hemolytic group A streptococci due to submininal inhibitory concentrations of chloramphenicol, erythromycin and penicillin. In: HOLM, S. E. & CHRISTENSEN, P., eds. Basic concepts of streptococci and streptococcal diseases Chertsey, England, Reedbooks, 1982. p. 148-150.

[8] 8. LANCEFIELD, R. C. A serological differentiation of human and other groups of hemolytic streptococci. J. exp. Med., 57: 571-595, 1933.

[9] 9. LORIAN, V. Some effects of subinhibitory concentrations of penicillin on the structure and division of staphylococci. Antimicrob. Agents Chemother., 7: 864-870, 1975.

[10] 10. LORIAN, V. Effects of subminimum inhibitory concentrations of antibiotics on bacteria. In: LORIAN, V., ed. Antibiotics in laboratory medicine Baltimore, Williams & Wilkins, 1980. p. 342-408.

[11] 11. LORIAN, V. & ATKINSON, B. Effects of subinhibitory concentrations of antibiotics on cross walls of cocci. Antimicrob. Agents Chemother., 6: 1043-1055, 1976.

[12] 12. MILATOVIC, D. Effect of subinhibitory antibiotic concentrations on the phagocytosis of Staphylococcus aureus. Europ. J. clin. Microbiol., 1: 97-101, 1982.

[13] 13. ROSENDAL, K. & FABER, V. Streptococcal hyaluronidase. III. The effect of penicillin on the production of hyluronic acid and hyaluronidase by hemolytic streptococci (type 24, group A). Acta pathol. microbiol. scand., 36: 263-273, 1955a.

[14] 14. ROSENDAL, K. & FABER, V. Streptococcal hyaluronidase. V. The effect of penicillin on the production of hyaluronic acid and hyaluronidase by hemolytic streptococci (type 28, group A). Acta patbol. microbiol. scand., 37: 293-299, 1955b.

[15] 15. VOSBECK, K.; METT, H.; HUBER, U.; BOHN, J. & PETIGNAT, M. Effects of low concentrations of antibiotics on Escherichia coll adhesion. Antimicrob. Agents Chemother., 21: 864-869, 1982.

[16] 16. WASHINGTON II, J. A. & BARRY, A. L. Dilution test procedures. In: LENNETTE, E. H.; SPAULDING, E. H. & TRUANT, J. P., eds. Manual of clinical microbiology Washington, D.C., American Society for Microbiology, 1974. p. 410-417.