Users of total prosthesis present in general a high frequency of the so called denture stomatitis, associated to erythematous candidiasis. So, we evaluated the susceptibility of oral yeast to three antifungal agents. Strains of Candida albicans isolated from patients with denture stomatitis were evaluated in relation to the susceptibility of the antifungal drugs as amphotericin B (polyenic derivatives), and azole agents as ketoconazole and miconazole. The antifungal activity was evaluated, and the minimal inhibitory concentration (MIC) and minimal fungicide concentration (MFC) were determined utilizing the agar dilution method. The results demonstrated low values for MICs and MFCs (0.15<FONT FACE="Symbol"> m</font>g/ml) for AmB in face of all yeast present. For miconazole and ketoconazole, MICs were invariably <FONT FACE="Symbol">£ </font>4.00<FONT FACE="Symbol"> m</font>g/ml, while values of the MFC were <FONT FACE="Symbol">³</font> 16.00<FONT FACE="Symbol"> m</font>g/ml for the majority of the strains. We could conclude that AmB presented a major fungicidal action in vitro, while azoles demonstrated a fungistatic but not fungicidal profile. So, we can consider that new drugs, mainly topical ones, are needed to treat lesions related to denture stomatitis, so commonly observed in wearers of dental prostheses.
Candidiasis, oral; Stomatitis, denture; Fungi; Antifungal agents; Amphotericin B; Azoles
