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Communications • Rev. Soc. Bras. Med. Trop. 45 (2) • Apr 2012 • https://doi.org/10.1590/S0037-86822012000200023   copy

Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis

Comparação entre três ensaios de reação em cadeia da polimerase na detecção de DNA de Leishmania, em amostras biológicas de pacientes com leishmaniose tegumentar americana

Authorship SCIMAGO INSTITUTIONS RANKINGS

INTRODUCTION: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. METHODS: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR). RESULTS: Results were evidenced by bands of approximately 120, 720, and 670 bp for L150/L152, 13Y/13Z, and snPCR, respectively. L150/L152, 13Y/13Z, and snPCR positivity indexes were 76.9, 56.4, and 9.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p<0.05), respectively, for confirmed. CONCLUSIONS: Preliminary results showed that these assays, mainly L150/L152 and snPCR, can detect Leishmania DNA and carry potential on laboratory diagnosis of leishmaniasis.

Leishmania; PCR; Diagnosis

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