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Viral etiology of acute respiratory infections in children in Southern Iran

Abstract

INTRODUCTION:

Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs).

METHODS:

Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV.

RESULTS:

Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%.

CONCLUSIONS:

The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.

Keywords:
Acute respiratory infections; Respiratory syncytial vírus; Influenza A vírus; Human metapneumovirus; Real-time polymerase chain reaction

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