Abstract
INTRODUCTION:
Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs).
METHODS:
Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV.
RESULTS:
Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%.
CONCLUSIONS:
The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.
Keywords:
Acute respiratory infections; Respiratory syncytial vírus; Influenza A vírus; Human metapneumovirus; Real-time polymerase chain reaction