Occurrence of <i>Blastocystis</i> spp. in domestic animals in Triângulo Mineiro area of Brazil

Moura, Renata Gregório Franco; Oliveira-Silva, Márcia Benedita de; Pedrosa, André Luiz; Nascentes, Gabriel Antônio Nogueira; Cabrine-Santos, Marlene

doi:10.1590/0037-8682-0484-2016

Short Communication • Rev. Soc. Bras. Med. Trop. 51 (02) • Mar-Apr 2018 • https://doi.org/10.1590/0037-8682-0484-2016 linkcopy

Occurrence of Blastocystis spp. in domestic animals in Triângulo Mineiro area of Brazil

Authorship SCIMAGO INSTITUTIONS RANKINGS

INTRODUCTION: Currently, there are few studies regarding Blastocystis epidemiology. This study aimed to evaluate the occurrence of Blastocystis in animals in Uberaba, Brazil.

METHODS: Fecal samples were examined by parasitological methods and screened for Blastocystis by polymerase chain reaction.

RESULTS: Blastocystis spp. were observed in pigs, sheep, cattle, and dogs. Blastocystis polymerase chain reaction was positive in 14/22 samples positive by parasitological methods.

CONCLUSIONS: The occurrence of Blastocystis in animals is high, with a predominance of subtype 1 in the region. This is the first study conducted in Brazil showing the genetic profile of Blastocystis isolated from animals.

Keywords:
Blastocystis spp; Domestic animals; Ritchie’s method

Host	Occurrence of	PR (CI95%)*	p-value**
	Blastocystis spp. n (%)
Pigs	13/18 (72.2)	1.00	< 0.01
Sheep	1/3 (33.3)	0.46 (0.09-2.35)
Cattle	6/28 (21.4)	0.30 (0.14-0.64)
Dogs	2/78 (2.6)	0.04 (0.01-0.14)
Cats	0/16 (0.0)	NA
Total	22/143 (15.4)

Ritchie	Direct microscopy
Ritchie	positive	negative	total
	n (%)	n (%)	n (%)
Positive	7 (4.9)	2 (1.4)	9 (6.3)
Negative	13 (9.1)	121 (84.6)	134 (93.7)
Total	20 (14.0)	123 (86.0)	143 (100.0)

Host	Positivity by parasitological methods (n_p)	PCR [n_PCR+/n_p]* (%)	ST1 genotypes (%)**
Dogs	2	0/2 (0.0)	ND
Cattle	6	5/6 (83.3)	0/5
Pigs	13	8/13 (61.5)	3/8 (37.5)
Sheep	1	1/1 (100.0)	0/1
Total	22	14/22 (63.6)	3/14 (21.4%)

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[TFN1] PR: prevalence ratio; CI95%: 95% confidence interval; NA: not applicable. *PR (CI95%) using the positivity of Blastocystis spp. in pigs as reference. **The p-value refers to the result of the chi-square test.