Hybrid capture II and PapilloCheck<sup>®</sup>
                    tests for detection of anal high-risk human papillomavirus

Maia, Livia Bravo; Marinho, Larissa Cardoso; Bocca, Anamélia Lorenzetti; Cavalcante Neto, Florêncio Figueiredo; Velasco, Lara Franciele Ribeiro; Costa, Patrícia Godoy Garcia; Carneiro, Fabiana Pirani; Oliveira, Paulo Gonçalves de

doi:10.1590/0037-8682-0081-2012

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Short Communications • Rev. Soc. Bras. Med. Trop. 47 (2) • Mar-Apr 2014 • https://doi.org/10.1590/0037-8682-0081-2012 copy

Hybrid capture II and PapilloCheck^® tests for detection of anal high-risk human papillomavirus

Authorship SCIMAGO INSTITUTIONS RANKINGS

Introduction

This study evaluated the level of concordance between hybrid capture II (HCII) and PapilloCheck^® for the detection of high-risk human papillomavirus (HPV) in anal samples.

Methods

Anal cell samples collected from 42 human immunodeficiency virus (HIV)+ patients were analyzed.

Results

Considering only the 13 high-risk HPV types that are detectable by both tests, HCII was positive for 52.3% of the samples, and PapilloCheck^® was positive for 52.3%. The level of concordance was 80.9% (Kappa = 0.61).

Conclusions

Good concordance was observed between the tests for the detection of high-risk HPV.

Human papillomavirus; PapilloCheck^®; Hybrid capture II

PapilloCheck^®	HCII		Total
PapilloCheck^®	positive	negative	Total
Positive	18	4	22
Negative	4	16	20
Total	22	20	42

Samples	RLU/CO values	SNRvalues
1	0.61	0
2	1.1	781.8
3	1.05	22.8
4	0.72	0
5	1.17	77.3
8	4.19	791
9	234.73	105
10	0.47	0
11	0.51	0
12	0.41	0
13	572.57	1180.1
14	0.33	0
16	1.02	57.6
17	51.7	321
18	0.51	0
19	2.52	292.1
20	0.35	0
21	0.46	0
22	14.64	574.5
23	5.01	502.3
24	0.35	0
25	0.63	0
26	3.29	550.3
28	0.26	0
29	0.5	0
30	10.39	574.5
31	0.52	0
32	10.85	235
33	45.35	886
34	0.32	0
35	1.86	153.5
37	457.94	365.7
39	457.94	2197
40	0.47	0

Samples	RLU/CO values	SNR values
6	1.38	0
7	4.19	0
15	0.51	91.1
27	0.63	21
36	0.3	198.1
38	16.52	0
41	0.48	29
42	3.52	0

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E-mail: rsbmt@uftm.edu.br

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[1] CONFLICT OF INTEREST The authors declare that there is no conflict of interest.

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Hybrid capture II and PapilloCheck® tests for detection of anal high-risk human papillomavirus

Introduction

Methods

Results

Conclusions

Hybrid capture II and PapilloCheck^® tests for detection of anal high-risk human papillomavirus