Alterations induced in the ileum of mice upon inoculation with different species of <i>Leishmania</i>: a preliminary study

Santos, Amanda Gubert Alves dos; Ferlini, Jéssica de Paula; Vicentino, Suellen Lais; Lonardoni, Maria Valdrinez Campana; Sant’Ana, Debora de Mello Gonçales; Melo, Gessilda de Alcântara Nogueira de

doi:10.1590/0037-8682-0348-2017

Short Communication • Rev. Soc. Bras. Med. Trop. 51 (04) • Jul-Aug 2018 • https://doi.org/10.1590/0037-8682-0348-2017 linkcopy

Alterations induced in the ileum of mice upon inoculation with different species of Leishmania: a preliminary study

Authorship SCIMAGO INSTITUTIONS RANKINGS

INTRODUCTION: Leishmania species cause skin, mucosal, and disseminated lesions. We studied the effects of three Leishmania species on ileal morphology in mice.

METHODS: BALB/c mice were intraperitoneally inoculated with Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis, and Leishmania (Leishmania) major (4 animals/group). After 72h, the ilea were collected and histologically processed.

RESULTS: Following inoculation, the goblet cell and intraepithelial lymphocyte populations increased, while Paneth cell number and crypt width decreased. In addition, enterocyte size, villi height, and mucosa, submucosa, and muscular tunic thickness increased.

CONCLUSIONS: Leishmania modified the quantity of cells in and morphology of mice ilea.

Keywords:
Leishmaniasis; Small intestine; Innate response

		CG	LLA	LLM	LVB
Enterocytes	Height (µm)	16.16±2.71^a	15.80±2.81^ab	14.30±2.40^c	16.64±2.72^d
	Width (µm)	4.40±0.90^a	4.30±0.96^ab	4.34±0.94^abc	4.60±0.96^d
Paneth cells	Number	87.33±12.23^a	53.75±10.42^b	68.33±16.63^abc	66.00±8.21^bc
	Area (µm²)	70.76±3.60^a	83.05±4.29^ab	68.39±3.60^abc	88.71±3.43^b
	Number of granules	10.37±5.68^a	11.62±7.33^ab	12.67±7.68^bc	13.35±7.30^c
	Area of granules (µm²)	21.16±1.32^a	23.82±1.61^ab	18.48±1.48^ac	25.38±1.24^b
Goblet cells	PAS	10.59±0.89^a	13.66±1.04^b	12.67±1.05^ab	14.08±1.06^b
	AB pH 1.0	11.06±0.26^a	12.09±0.11^ab	12.73±0.15^b	11.65±0.49^ab
	AB pH 2.5	11.08±0.11^a	13.69±0.49^b	13.21±0.58^b	12.50±0.28^b
IELs		0.48±0.07^a	1.11±0.13^b	0.99±0.13^b	0.98±0.04^b

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[TFN1] Mean ± standard error followed by different letters (a-d) in the same row indicate significant differences (p < 0.05, ANOVA). n = 4 animals/group. CG: control group; LLA: Leishmania (Leishmania) amazonensis; LLM: Leishmania (Leishmania) major; LVB: Leishmania (Viannia) braziliensis; IELs: intraepithelial lymphocytes; PAS: periodic acid Schiff; AB: Alcian blue; ANOVA: analysis of variance.