Alterações quantitativas das células neuroendócrinas no Megacólon chagásico

Barbosa, Alfredo J.A.; Toppa, Nivaldo H.; Melo, José R. Cunha; Tafuri, Washington L.

doi:10.1590/S0037-86821985000400002

Resumos

Utilizando-se técnicas histoquímicas pela prata (Masson-Fontana e Sevier- Munger) estudaram-se a distribuição e o número de células enterocromafins (EC) e do conjunto EC mais células argirófilas (ARG) em peças cirúrgicas de megassigmóide chagásico. Observou-se que tanto nos "megas"(n = 16) como nos controles (n = 8), a posição das células EC e ARG na mucosa foi predominantemente basal, com distribuição irregular, principalmente das EC. A análise histológica revelou aparente aumento numérico das EC e do conjunto EC mais ARG. Todavia, contagens sistematizadas mostraram que somente o conjunto dessas células apresentou aumento estatisticamente significativo (P < 0,01). Realizou-se também o estudo morfométrico da mucosa e das musculares que revelou espessamento significativo (P

Megacólon chagásico; Células enterocromafins; Células argirófilas; Células neuroendócrinas

Silver stainings (Masson-Fontana and Sevier- Munger methods) were applied to sections from surgical specimens of chagasic megasigmoid. The distribution and the number of both enterochromaffin (EC) cells and EC plus argyrophyl (ARG) cells were studied. Both of them, EC and ARG cells presented a predominantly basal localization in mucosal glands of megasigmoids (n = 16) and Controls (n= 8) The counting of EC cells and EC plus ARG cells has shown that only the group EC plus ARG cells was significantly increased. Morphometric studies revealed a significant increase in the thickness of the muscle and mucosa layers of the megasigmoid when compared to the Controls.

Chagasic megacolon; Enterochromaffin cells; Argyrophyl cells; Neuroendocrine cells

ARTIGOS

Alterações quantitativas das células neuroendócrinas no Megacólon chagásico^* * Trabalho realizado no Departamento de Anatomia Patológica e Medicina Legal da Faculdade de Medicina da Universidade Federal de Minas Gerais. Av. Alfredo Balena 190, 30.000, Belo Horizonte, MG. Brasil. Auxilio financeiro: CNPq(Proc. nP 30.1851/76) e FINEP (Proc. n o 43/83/0119/00).

Alfredo J.A. Barbosa; Nivaldo H. Toppa; José R. Cunha Melo; Washington L. Tafuri

RESUMO

Utilizando-se técnicas histoquímicas pela prata (Masson-Fontana e Sevier- Munger) estudaram-se a distribuição e o número de células enterocromafins (EC) e do conjunto EC mais células argirófilas (ARG) em peças cirúrgicas de megassigmóide chagásico. Observou-se que tanto nos "megas"(n = 16) como nos controles (n = 8), a posição das células EC e ARG na mucosa foi predominantemente basal, com distribuição irregular, principalmente das EC. A análise histológica revelou aparente aumento numérico das EC e do conjunto EC mais ARG. Todavia, contagens sistematizadas mostraram que somente o conjunto dessas células apresentou aumento estatisticamente significativo (P < 0,01). Realizou-se também o estudo morfométrico da mucosa e das musculares que revelou espessamento significativo (P <0,01) dessas camadas em relação aos casos controles.

Palavras-chave: Megacólon chagásico. Células enterocromafins. Células argirófilas. Células neuroendócrinas.

ABSTRACT

Silver stainings (Masson-Fontana and Sevier- Munger methods) were applied to sections from surgical specimens of chagasic megasigmoid. The distribution and the number of both enterochromaffin (EC) cells and EC plus argyrophyl (ARG) cells were studied. Both of them, EC and ARG cells presented a predominantly basal localization in mucosal glands of megasigmoids (n = 16) and Controls (n= 8) The counting of EC cells and EC plus ARG cells has shown that only the group EC plus ARG cells was significantly increased. Morphometric studies revealed a significant increase in the thickness of the muscle and mucosa layers of the megasigmoid when compared to the Controls.

Keywords: Chagasic megacolon. Enterochromaffin cells. Argyrophyl cells. Neuroendocrine cells.

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1. Ali-Rachedi A, Varndell IM, Adrian TE, Gapp DA, VanNooden S, Bloom SR. Polak JM. Peptide YY(PYY) immunoreactivity is eostored with glucagon-related immunoreactants in endocrine cells of the gut and pancreas. Histochemistry 80: 487-491, 1984.
2. Alumets J, Hãkanson R, Sundler F, Chang KJ. Leu- Enkephalin-like material in nerves and enterochromafíin cells in the gut. Histochemistry 56: 187-196, 1978.
3. Barbosa AJA, Castro, LPF, Nogueira AMMF. A simple and economical modification of the Masson-Fontana method for staining melanin granules and enterochromaffin cells. Stain Technology 59: 193-196, 1984.
4. Buffa R, Polak JM, Pearse AGE, Solcia E, Grimelius L, Capella C. Identification of the intestinal cell storing gastric inhibitory peptide. Histochemistry 43: 249-255, 1975.
5. Buffa R, Capella C, Fontana P, Usellini L, Solcia E. Types of endocrine cells in the human colon and rectum. Cell Tissue Research 192: 227-240, 1978.
6. Bülbring E, Lin RCY. The effect of intraluminal application of 5-hydroxytryptamine and 5-hydroxy- tryptophan on peristalsis; the local production of 5-HT and its release in relation to intraluminal pressure and propulsive activity. Journal of Physiology (London) 140: 381-407, 1958.
7. Bòttcher G, Sjõlund K, Ekblad E, Hakanson R, Schwartz TW, Sundler F. Coexistence of peptide YY and glicetin immunoreactivity in endocrine cells of the gut. Regulatory Peptides 8: 261-266, 1984.
8. Costa M. Fumess JB. Coelho AC, Verhofstad AAJ, Steinbusch HWJ, Elde RP. Neurons with 5-hydroxy- tryptamine like immunoreactivity in the enteric nervous system: their visualization and reactions to drug treatment. Neuroscience 2: 351-363, 1982.
9. Facer P, Polak JM, Jaffe BM, Pearse AGE. Immunocytochemical demonstration of 5-hydroxytyptamine in gastrointestinal endocrine cells. Histochemical Journal 11: 117-121, 1979.
10. Fahrenkrug J. Vasoactive Intestinal Polypeptide: measurement, distribution and putative neurotransmitter function. Digestion 19: 149-169, 1979.
11. Fath S, Hedlund H, Hultén L, Nordgren S, Oresland T. The effects of 5-hydroxytryptamine on large intestinal motility and blood flow in the cat. Acta Physiologica Scandinavica 118: 329-336, 1983.
12. Fetissof F, Desbois MP, Assan R, Arbeille-Brassart, Baraudi A, Tharanne MJ, Jobard P. Endocrine cells in the anal canal. Virchows Archives (Pathological Anatomy and Histopathology) 404: 39-47, 1984.
13. Ferri G-L, AdrianTE, Mohammad AG, O'Shaughnessy DJ, Probert L, Lee YC, Buchan AMJ, Polak JM, Bloom SR. Tissue localization and relative distribution of regulatory peptides in separated layers from the human bowel. Gastroenterology 84: 777-786, 1983.
14. Fontana A. Verfahren zur intensiven und raschen Farbung des Treponema pallidum und anderer Spirochäten. Dermatologische Wochenschrift. 55: 1003- 1004, 1912.
15. Fujita T, Kobayashi S. Paraneural cells in GEP endocrine system. In: Bloom SR(ed.) GutHormones, lsted. Churchill Livingstone, London, p. 414-422, 1978.
16. Fumers JB, Costa M. Types of nerves in the enteric nervous system. Neuroscience 5: 1-20, 1980.
17. Grimelius L, Wilander E. Silver stains in the study of endocrine cells of the gut and pancreas. Investigative Cell Pathology 3: 3-12, 1980.
18. Heitz PU, Kasper M, Krey G, Polak JM, Pearse AGE. Immunoelectron cytochemical localization of motilin in human duodenal enterochromaffin cells. Gastroenterology 74: 713-717, 1978.
19. Inokuchi H, Kawai K, Takeuchi Y, Sano Y. Immunohistochemical demonstration of EC cells in rat gastrointestinal tract. Histochemistry 74: 453-456, 1983.
20. Johnson CR. New aspects of the trophic action of gastrointestinal hormones. Gastroenterology 72: 788- 792, 1977.
21. Köberle, F. Megacolon. Journal of Tropical Medicine and Hygiene 61: 21-24, 1958.
22. Köberle, F. Patogenia do megaesôfago brasileiro e europeu. Revista Goiana de Medicina 9: 79-116, 1963.
23. Kurian SS, Ferri G-L, De Mey J, Polak JM. Immuno- cytochemistry of serotonin-containing nerves in the human gut. Histochemistry 78: 523-529, 1983.
24. Larsson LI, Goltermann N, De Magistries L, Rehfeld JF, Schwartz TW. Somatostatin cell processes as pathways for paracrine secretion. Science 205: 1393- 1395, 1979.
25. Lindop GBM. Enterochromaffin cell hyperplasia and megacolon: report of a case. Gut 24: 575-578, 1983.
26. Long RG, Bishop AE, Barnes AJ, Albuquerque RH, 0'Shaughnessy DJ, McGregor GP, Bannister R, Polak JM, Bloom SR. Neural and hormonal peptides in rectal biopsy specimens from patients with Chagas' disease and chronic autonomic failure. Lancet 1: 559-562, 1980.
27. Masson MP. La glande endocrine de Fintestin chez Phomme. Comptes Rendus Hebdomadaires des Séances de PAcademic des Sciences 158: 59-61, 1914.
28. Pearse AGE, Polak JM. Immunocytochemical localization of substance P in mammalian intestine. Histochemistry 41: 373-375, 1975.
29. Resmick RH. Gray SJ. Distribution of serotonin (5- hydroxytryptamine) in the human gastrointestinal tract. Gastroenterology 41: 119-121, 1961.
30. Sevier AC, Munger BL. A silver method for paraffin sections of neural tissue. Journal of Neuropathology and Experimental Neurology 24: 130-135, 1965.
31. Sjõlund K, Sandén G, Hakanson R, Sundler F. Endocrine cells in human intestine: an immunocytochemical study. Gastroenterology 85: 1120-1130, 1983.
32. Solcia E, Polak JM, Larsson, L-I, Buchan AMJ, Capella C. Update on Lausanne classification of endocrine cells. In: Bloom SR, Polak JM, (eds.) Gut Hormones. 2nd ed. Churchill Livingstone, London, p. 98-100, 1981.
33. Solcia E, Capella C, Buffa R, Frigerio B, Usellini L, Fiocca R. Morphological and functional classification of endocrine cells and related growths in the gastrointestinal tract. In: Class GBJ (ed.) Gastrointestinal Hormones, Raven Press, New York, p. 1-17, 1980.
34. Solcia E, Capella C, Buffa RE, Usellini L, Fiocca R, Frigerio B, Tenti P, Sessa F. The diffuse endocrine paracrine system of the gut in health and disease: Ultrastructural features. In: Polak JM. Bloom SR, Wright NA, Daly MJ (ed.) Structure of the gut. Glaxo Group Research Ltd. Ware, Herts, UK, 1982.
35. Tafuri WL, Maria TA, Lopes ER. Alterações das células e dos componentes do interstício no megaesôfago na tripanosomíase cruzi humana. Estudo ao microscópio eletrônico. Revista da Associação Médica de Minas Gerais. 24: 1-24, 1973.
36. Tafuri, WL. Doença de Chagas. Anais do Instituto de Ciências Exatas e Biológicas da Universidade Federal de Ouro Preto 1: 118-150, 1983.
37. Tatemoto K. Isolation and characterization of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. Proceedings ofNational Academy of Sciences USA 79: 2514-2518, 1982.
38. Vassalo G, Capella C, Solcia E. Endocrine cells of the human gastric mucosa. Zeitschrift für Zellforschung und Mikroskopische Anatomie 118: 49-67, 1971.
39. Verity MA, Mellinkoff SM, Frankland M, Greipel M. Serotonin content and argentaffm and Paneth cell changes in ulcerative colitis. Gastroenterology 43: 24- 31, 1962.
40. Willander E, Lundqvist M, Westermark P, Grimelius L. Immunocytochemical study of argyrophil (Sevier Munger) endocrine cells of adult human pancreas. Histochemistry 68: 95-98, 1980.

*

Trabalho realizado no Departamento de Anatomia Patológica e Medicina Legal da Faculdade de Medicina da Universidade Federal de Minas Gerais. Av. Alfredo Balena 190, 30.000, Belo Horizonte, MG. Brasil.

Auxilio financeiro: CNPq(Proc. nP 30.1851/76) e FINEP (Proc. n

^o 43/83/0119/00).

Datas de Publicação

Publicação nesta coleção
07 Jun 2013
Data do Fascículo
Dez 1985

Histórico

Recebido
07 Dez 1984
Aceito
07 Dez 1984

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Ali-Rachedi A, Varndell IM, Adrian TE, Gapp DA, VanNooden S, Bloom SR. Polak JM. Peptide YY(PYY) immunoreactivity is eostored with glucagon-related immunoreactants in endocrine cells of the gut and pancreas. Histochemistry 80: 487-491, 1984.

[2] 2. Alumets J, Hãkanson R, Sundler F, Chang KJ. Leu- Enkephalin-like material in nerves and enterochromafíin cells in the gut. Histochemistry 56: 187-196, 1978.

[3] 3. Barbosa AJA, Castro, LPF, Nogueira AMMF. A simple and economical modification of the Masson-Fontana method for staining melanin granules and enterochromaffin cells. Stain Technology 59: 193-196, 1984.

[4] 4. Buffa R, Polak JM, Pearse AGE, Solcia E, Grimelius L, Capella C. Identification of the intestinal cell storing gastric inhibitory peptide. Histochemistry 43: 249-255, 1975.

[5] 5. Buffa R, Capella C, Fontana P, Usellini L, Solcia E. Types of endocrine cells in the human colon and rectum. Cell Tissue Research 192: 227-240, 1978.

[6] 6. Bülbring E, Lin RCY. The effect of intraluminal application of 5-hydroxytryptamine and 5-hydroxy- tryptophan on peristalsis; the local production of 5-HT and its release in relation to intraluminal pressure and propulsive activity. Journal of Physiology (London) 140: 381-407, 1958.

[7] 7. Bòttcher G, Sjõlund K, Ekblad E, Hakanson R, Schwartz TW, Sundler F. Coexistence of peptide YY and glicetin immunoreactivity in endocrine cells of the gut. Regulatory Peptides 8: 261-266, 1984.

[8] 8. Costa M. Fumess JB. Coelho AC, Verhofstad AAJ, Steinbusch HWJ, Elde RP. Neurons with 5-hydroxy- tryptamine like immunoreactivity in the enteric nervous system: their visualization and reactions to drug treatment. Neuroscience 2: 351-363, 1982.

[9] 9. Facer P, Polak JM, Jaffe BM, Pearse AGE. Immunocytochemical demonstration of 5-hydroxytyptamine in gastrointestinal endocrine cells. Histochemical Journal 11: 117-121, 1979.

[10] 10. Fahrenkrug J. Vasoactive Intestinal Polypeptide: measurement, distribution and putative neurotransmitter function. Digestion 19: 149-169, 1979.

[11] 11. Fath S, Hedlund H, Hultén L, Nordgren S, Oresland T. The effects of 5-hydroxytryptamine on large intestinal motility and blood flow in the cat. Acta Physiologica Scandinavica 118: 329-336, 1983.

[12] 12. Fetissof F, Desbois MP, Assan R, Arbeille-Brassart, Baraudi A, Tharanne MJ, Jobard P. Endocrine cells in the anal canal. Virchows Archives (Pathological Anatomy and Histopathology) 404: 39-47, 1984.

[13] 13. Ferri G-L, AdrianTE, Mohammad AG, O'Shaughnessy DJ, Probert L, Lee YC, Buchan AMJ, Polak JM, Bloom SR. Tissue localization and relative distribution of regulatory peptides in separated layers from the human bowel. Gastroenterology 84: 777-786, 1983.

[14] 14. Fontana A. Verfahren zur intensiven und raschen Farbung des Treponema pallidum und anderer Spirochäten. Dermatologische Wochenschrift. 55: 1003- 1004, 1912.

[15] 15. Fujita T, Kobayashi S. Paraneural cells in GEP endocrine system. In: Bloom SR(ed.) GutHormones, lsted. Churchill Livingstone, London, p. 414-422, 1978.

[16] 16. Fumers JB, Costa M. Types of nerves in the enteric nervous system. Neuroscience 5: 1-20, 1980.

[17] 17. Grimelius L, Wilander E. Silver stains in the study of endocrine cells of the gut and pancreas. Investigative Cell Pathology 3: 3-12, 1980.

[18] 18. Heitz PU, Kasper M, Krey G, Polak JM, Pearse AGE. Immunoelectron cytochemical localization of motilin in human duodenal enterochromaffin cells. Gastroenterology 74: 713-717, 1978.

[19] 19. Inokuchi H, Kawai K, Takeuchi Y, Sano Y. Immunohistochemical demonstration of EC cells in rat gastrointestinal tract. Histochemistry 74: 453-456, 1983.

[20] 20. Johnson CR. New aspects of the trophic action of gastrointestinal hormones. Gastroenterology 72: 788- 792, 1977.

[21] 21. Köberle, F. Megacolon. Journal of Tropical Medicine and Hygiene 61: 21-24, 1958.

[22] 22. Köberle, F. Patogenia do megaesôfago brasileiro e europeu. Revista Goiana de Medicina 9: 79-116, 1963.

[23] 23. Kurian SS, Ferri G-L, De Mey J, Polak JM. Immuno- cytochemistry of serotonin-containing nerves in the human gut. Histochemistry 78: 523-529, 1983.

[24] 24. Larsson LI, Goltermann N, De Magistries L, Rehfeld JF, Schwartz TW. Somatostatin cell processes as pathways for paracrine secretion. Science 205: 1393- 1395, 1979.

[25] 25. Lindop GBM. Enterochromaffin cell hyperplasia and megacolon: report of a case. Gut 24: 575-578, 1983.

[26] 26. Long RG, Bishop AE, Barnes AJ, Albuquerque RH, 0'Shaughnessy DJ, McGregor GP, Bannister R, Polak JM, Bloom SR. Neural and hormonal peptides in rectal biopsy specimens from patients with Chagas' disease and chronic autonomic failure. Lancet 1: 559-562, 1980.

[27] 27. Masson MP. La glande endocrine de Fintestin chez Phomme. Comptes Rendus Hebdomadaires des Séances de PAcademic des Sciences 158: 59-61, 1914.

[28] 28. Pearse AGE, Polak JM. Immunocytochemical localization of substance P in mammalian intestine. Histochemistry 41: 373-375, 1975.

[29] 29. Resmick RH. Gray SJ. Distribution of serotonin (5- hydroxytryptamine) in the human gastrointestinal tract. Gastroenterology 41: 119-121, 1961.

[30] 30. Sevier AC, Munger BL. A silver method for paraffin sections of neural tissue. Journal of Neuropathology and Experimental Neurology 24: 130-135, 1965.

[31] 31. Sjõlund K, Sandén G, Hakanson R, Sundler F. Endocrine cells in human intestine: an immunocytochemical study. Gastroenterology 85: 1120-1130, 1983.

[32] 32. Solcia E, Polak JM, Larsson, L-I, Buchan AMJ, Capella C. Update on Lausanne classification of endocrine cells. In: Bloom SR, Polak JM, (eds.) Gut Hormones. 2nd ed. Churchill Livingstone, London, p. 98-100, 1981.

[33] 33. Solcia E, Capella C, Buffa R, Frigerio B, Usellini L, Fiocca R. Morphological and functional classification of endocrine cells and related growths in the gastrointestinal tract. In: Class GBJ (ed.) Gastrointestinal Hormones, Raven Press, New York, p. 1-17, 1980.

[34] 34. Solcia E, Capella C, Buffa RE, Usellini L, Fiocca R, Frigerio B, Tenti P, Sessa F. The diffuse endocrine paracrine system of the gut in health and disease: Ultrastructural features. In: Polak JM. Bloom SR, Wright NA, Daly MJ (ed.) Structure of the gut. Glaxo Group Research Ltd. Ware, Herts, UK, 1982.

[35] 35. Tafuri WL, Maria TA, Lopes ER. Alterações das células e dos componentes do interstício no megaesôfago na tripanosomíase cruzi humana. Estudo ao microscópio eletrônico. Revista da Associação Médica de Minas Gerais. 24: 1-24, 1973.

[36] 36. Tafuri, WL. Doença de Chagas. Anais do Instituto de Ciências Exatas e Biológicas da Universidade Federal de Ouro Preto 1: 118-150, 1983.

[37] 37. Tatemoto K. Isolation and characterization of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. Proceedings ofNational Academy of Sciences USA 79: 2514-2518, 1982.

[38] 38. Vassalo G, Capella C, Solcia E. Endocrine cells of the human gastric mucosa. Zeitschrift für Zellforschung und Mikroskopische Anatomie 118: 49-67, 1971.

[39] 39. Verity MA, Mellinkoff SM, Frankland M, Greipel M. Serotonin content and argentaffm and Paneth cell changes in ulcerative colitis. Gastroenterology 43: 24- 31, 1962.

[40] 40. Willander E, Lundqvist M, Westermark P, Grimelius L. Immunocytochemical study of argyrophil (Sevier Munger) endocrine cells of adult human pancreas. Histochemistry 68: 95-98, 1980.