Acessibilidade / Reportar erro

Sporulation methodology for fungi of the Botryosphaeriaceae family

ABSTRACT

Fungi belonging to the Botryosphaeriaceae family are potential phytopathogens of considerable importance in plant disease studies due to their cosmopolitan, parasitic and endophytic habit, and great genetic, morphological and ecological diversity. The difficulty with sporulation and the long periods for such step in the reproductive cycle are the major limitations of handling these fungi tlid-translation in vitrotlid-translation. Thus, the present study aimed to test sugar apple fruits (Annona squamosa) as an alternative culture medium to obtain sporulation periods shorter than those described in the literature. Finally, the use of sugar apple fruits as a culture medium proved to be efficient for sporulation of the tested isolates. In addition, two of the evaluated isolates were only capable of sporulating in sugar apple fruits .

Keywords
Annona squamosa ; ecological diversity; culture medium

Grupo Paulista de Fitopatologia FCA/UNESP - Depto. De Produção Vegetal, Caixa Postal 237, 18603-970 - Botucatu, SP Brasil, Tel.: (55 14) 3811 7262, Fax: (55 14) 3811 7206 - Botucatu - SP - Brazil
E-mail: summa.phyto@gmail.com