Alternaria dauci and A. solani are fungal species known for h and 0 h / 24 h) were tested. The method developed in this study was difficult sporulation in culture media. This study had the objective of compared to the traditional procedure (BDA, 25 ºC, 12 h white / 12 verifying the influence of some physical factors on mycelia growth h dark light and scratching of the colony). V8-agar medium, and sporulation of these two species. Culture media (BDA, oat and temperature of 25 ºC, NUV light and scratching of the colonies had a V8), temperature (15ºC, 20ºC, 25ºC, 30ºC and 35ºC), light wavelengths marked effect on growth and sporulation. The 12 h light / 12 h dark during incubation (yellow, blue, white, NUV, green, and red), injury photoperiod stimulated sporulation most. In general, longer dark types applied to the colony (scratching, UV, microwave irradiation, than light periods after the injury of the colony favored sporulation. and temperature of 100 ºC) and photoperiods (light / dark, respectively, The developed procedure is clearly superior to the traditional method of 24 h / 0 h, 22 h / 2 h, 17 h / 7 h, 12 h / 12 h, 7 h / 17 h, 2 h / 22 for growth and sporulation of both species.
Phytopathogenic fungi; inoculum production; light; temperature
