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Influence of culture media and the carbon-nitrogen interaction on growth and sporulation of Penicillium sclerotigenum

The yam green rot is a disease with high economic impact in Pernambuco state. It was carried out physiological studies involving six culture media (PDA, Oat-agar, V-8 juice, Czapeck, Carrot and Yam) on mycelial growth, sporulation and dry weight of two Penicillium sclerotigenum isolates (IB1 and IPR2). PDA and Yam media induced higher mycelial growth and higher conidium production was obtained in the Czapeck and Oat-agar for both isolates utilized. The Oat-agar media provided higher dry weight for IB1 and IPR2. It was also studied the effect of different carbon source (maltose, dextrose, sucrose, starch) and nitrogen (asparagin, pepton, sodium nitrate, potassium nitrate) on development of P. Sclerotigenum. Among the nitrogen sources evaluated, sodium nitrate was the less favorable for the mycelial growth and sporulation. The sucrose x pepton combination provided the best mycelial growth for both isolates. The highest conidium production was achieved using the combination of sucrose x potassium nitrate and sucrose x peptone for the isolates IB1 and IPR2, respectively.

Green rot; Dioscorea cayennensis; postharvest; nutrition


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