ABSTRACT
The aim of this study was to verify the potential of extracts derived from medicinal plants (Rosmarinus officinalis L., Equisetum arvense L. and Moringa oleifera Lam.) and commercial products (based on plant extracts, plant oil and potassium phosphite) on the in vitro control of Diplocarpon rosae. The effect of treatments on the phytopathogen was evaluated according to the mycelial growth speed rate, mycelial growth inhibition, sporulation inhibition, sporulation rate, protein synthesis, and peroxidase and catalase specific activity on the mycelium of the phytopathogen, using aqueous crude extract (ACE) of R. officinalis leaves (1%); ACE of E. arvense stems (1%); ACE of M. oleifera seeds (1%); commercial product based on fermented plant extracts (0.01%); commercial product based on potassium phosphite (0.01%); commercial product based on plant oil (0.01%); product based on citrus mass (0.01%); fungicide based on thiophanate methyl (0.007%); and adjuvant (0.0025%) in PDA and PD media. Results showed a higher reduction in the growth of D. rosae in the presence of commercial product based on plant oils. The ACE of E. arvense and the commercial product based on fermented plant extracts exhibited anti-sporulant effect. Treatments with ACE of R. officinalis, ACE of E. arvense, ACE of M. oleifera, commercial product based on plant oil and product based on citrus mass increased protein synthesis in D. rosae mycelium. There was increased specific activity of the enzymes peroxidase and catalase in the mycelium in the presence of ACE of R. officinalis and commercial product based on potassium phosphite, respectively. The ACE of E. arvense and the commercial products based on plant oils and fermented plant extracts showed promising results in the control of the phytopathogen D. rosae.
Keywords
alternative control; black spot; enzymes
